Bacillus subtilis from Potato Rhizosphere as Biological Control Agent and Chili Growth Promoter

https://doi.org/10.22146/jpti.40606

Nur Prihatiningsih(1*), Heru Adi Djatmiko(2), Erminawati Erminawati(3), Puji Lestari(4)

(1) Faculty of Agriculture, Universitas Jenderal Soedirman, Jln. dr. Suparno No. 61 Purwokerto, Central Java 53123
(2) Faculty of Agriculture, Universitas Jenderal Soedirman, Jln. dr. Suparno No. 61 Purwokerto, Central Java 53123
(3) Faculty of Agriculture, Universitas Jenderal Soedirman, Jln. dr. Suparno No. 61 Purwokerto, Central Java 53123
(4) Faculty of Mathematics and Science, Universitas Jenderal Soedirman Jln. dr. Suparno No. 61 Purwokerto, Central Java 53123
(*) Corresponding Author

Abstract


Bacillus subtilis is an antagonist bacteria  that inhibits the growth of fungal and bacterial plant pathogens. The B. subtilis has roles as biocontrol agents and plant growth promoting rhizobacteria (PGPR). This research aimed to evaluate the potency of B. subtilis isolates (B209, B211, and B298) as a biocontrol agent to anthracnose (caused by Colletotrichum spp.) and as PGPR to increase the growth of chili plants. The experiments were divided into two batches. The first batch was conducted in the laboratory to evaluate the characteristics of B. subtilis (as biocontrol) and PGPR (phosphate solubility, producing IAA and nitrogen). The second batch was conducted in the field in Rempoah Village, Baturraden Regency, Banyumas District, with 5 treatments and 6 replications. Parameters observed were inhibition percentage to Colletotrichum spp., disease intensity, the component of PGPR, plant growth of chili, and phatosystem. The results showed that B209, B211, and B298 isolates inhibited the growth of Colletotrichum spp, with the highest inhibition percentage on B298. B209, B211, and B298 have characteristics as PGPR, i.e. the ability to soluble phosphate, to produce IAA and nitrogen. The ability of B298 to promote plant growth was shown by the increase of plant height, leaf number, plant dry mass, and dry root mass (38.0%, 54.7%, 61.7%, 61.8%, respectively). B298 and B211 could increase the fresh crop mass (41.2% and 37.1%) and fresh root mass (36.4% and 34.4%). B298 and B209 were similar in increasing the root length (25.2%). Root volume could be increased by 33.3% by applying B211 isolate. B209 was the best isolate to reduce anthracnose up to 80.36%.

Keywords


Bacillus subtilis; biocontrol agent; chili growth; PGPR

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References

Adesemoye, A.O., H.A. Torbert, & J.W. Kloepper. 2009. Plant Growth-promoting Rhizobacteria Allow Reduced Application Rates of Chemical Fertilizers. Microbial Ecology 58: 921–929.

Ahmad, M. & M. Kibret. 2014. Mechanisms and Applications of Plant Growth Promoting Rhizobacteria: Current Perspective. Journal of King Saud University - Science 26: 1–20.

Amin, M. & T.H. Flowers. 2004. Evaluation of Kjeldahl Digestion Method. Journal of Research (Science) 15: 159–179.

Azizah, S. N., N.R. Mubarik, & L.I. Sudirman. 2015. Potential of Chitinolytic Bacillus amyloliquefaciens SAHA 12.07 and Serratia marcescens KAHN 15.12 as Biocontrol Agents of Ganoderma boninense. Research Journal of Microbiology 10: 452–465.

Chaiharn, M. C., A. Kozo, & S. Lumyong. 2008. Screening of Rhizobacteria for their Plant Growth Promoting Activities. KMITL Science and Technology Journal 8: 18–23.

Compant S., B. Duffy, J. Nowak, C. Cle´ment, & E.A. Barka. 2005. Use of Plant Growth-promoting Bacteria for Biocontrol of Plant Diseases: Principles, Mechanism of Action, and Future Prospects. Applied and Environmental Microbiology 71: 4951–4959.

Compant, S., C. Clément, & A. Sessitsch. 2010. Plant Growth-promoting Bacteria in the Rhizo- and Endosphere of Plants: Their Role, Colonization, Mechanisms Involved and Prospects for Utilization. Soil Biology and Biochemistry 42: 669–678.

Dawwam, G. E., A. Elbeltagy, H.M. Emara, I.H. Abbas, & M.M. Hassan. 2013. Beneficial Effect of Plant Growth Promoting Bacteria Isolated from the Roots of Potato Plant. Annals of Agricultural Sciences 58: 195–201.

de Freitas, J.R., M.R. Banerjee, J.J. Germida. 1997. Phosphate-solubilizing Rhizobacteria Enhance the Growth and Yield but Not Phosphorus Uptake of Canola (Brassica napus L.). Biology and Fertility of Soils 24: 358–364.

Dwimartina, F.,T. Arwiyanto, & T. Joko. 2017. Potential of Endophytic and Rhizobacteria as an Effective Biocontrol for Ralstonia syzygii. Asian Journal of Plant Pathology 11: 191-198

Gupta, G. N. Astava, S.K. Khare, & V. Prakash. 2014. Role of Phosphate Solubilizing Bacteria in Crop Growth and Disease Management. Journal of Pure and Applied Microbiology 8: 461–474.

Kesaulya, H., Baharuddin, & I. Zakaria. 2015. Isolation and Physiological Characterization of PGPR from Potato Plant Rhizosphere in Medium Land of Buru Island. Procedia Food Science 3: 190–199.

Lestari P, N. Prihatiningsih, & H.A. Djatmiko. 2017. Partial Biochemical Characterization of Crude Extract Extracellular Chitinase Enzyme from Bacillus subtilis B 298. IOP Conference Series: Materials Science and Engineering 172: 012041.

Mishra, V.K. & A. Kumar. 2012. Plant Growth Promoting and Phytostimulatory Potential of Bacillus subtilis and Bacillus amyloliquefaciens. ARPN Journal 7: 509–519.

Paramita, N.R., C. Sumardiyono, & Sudarmadi. 2014. Pengendalian Kimia dan Ketahanan Colletotrichum spp. terhadap Fungisida Simoksanil pada Cabai Merah. Jurnal Perlindungan Tanaman Indonesia 18: 41–46.

Saharan, B.S. & V. Nehra. 2011. Plant Growth Promoting Rhizobacteria : A Critical Review. Life Sciences 2011: 1–30.

Shrivastava, U.P. & A. Kumar. 2011. A Simple and Rapid Plate Assay for the Screening of Indole-3-acetic Acid (IAA) Producing Microorganisms. International Journal of Applied Biology and Pharmaceutical Technology 2: 120–123.

Wang, H.K., R.F. Xiao & Q. Wei. 2013. Antifungal Activity of Bacillus coagulans TQ33 , Isolated from Skimmed Milk Powder against Botrytis cinerea. Food Technology and Biotechnology 51: 78–83.

Xie, G.H., M.Y Cai, G.C. Tao, & Y. Steinberger. 2003. Cultivable Heterotrophic N2-fixing Bacterial Diversity in Rice Fields in the Yangtze River Plain. Biology and Fertility of Soils 37: 29–38.



DOI: https://doi.org/10.22146/jpti.40606

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