* Corresponding Author
Detection and Identification of Banana-associated Phytoplasma Using Nested-PCR Method
https://doi.org/10.22146/jpti.38320 Saurma Mona Astrid Sibarani(1), Tri Joko(2), Siti Subandiyah(3*)
(1) Indonesian Agricultural Quarantine Agency, Ministry of Agriculture Jln. Harsono RM No. 3 Ragunan, Jakarta Selatan, Jakarta 12560
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
Phytoplasma is known to be associated with plant diseases in about 300 plant species from various families. Information on the presence of phytoplasma in bananas as one of the pathogens that can cause disease in bananas in Indonesia has never been reported. This research was conducted with the aim to detect the presence of banana phytoplasma by the nested-PCR method and to identify phytoplasma obtained based on the sequence analysis of the 16S rRNA gene. Standard PCR was carried out using P1/P7 primary pairs, followed by nested-PCR using a pair of R16F2n/R16R2m23SR primers separately that could amplify the target 16S rRNA genes in a row at 1600 bp. BLAST analysis shows that the results of phylogenetic analysis of banana phytoplasmic nucleotide cv. manggala from Tasikmalaya and cv. Raja nangka from Banjar has a genetic relationship that is closer to lethal wilt oil palm Phytoplasma (Candidatus Phytoplasma asteris). This phytoplasma belongs to the 16SrI-B group (aster yellows).
Keywords
Full Text:
PDFReferences
Agrios, G.N. 2005. Plant Pathology. Elsevier Academic Press, USA. 922 p.
Alhudaib, K. & A.A. Rezk. 2014. Molecular Characterization of Phytoplasm-Associated Disease in Tomato (Lycopersicum esculentum L.) in Saudi Arabia. International Journal of Virology 10: 10−191.
Al-Saleh, M. A. & M.A. Amer. 2014. Molecular Characterization of the 16SR II Group of Phytoplasm Associated with Faba Bean (Vicia faba L.) in Saudi Arabia. The Journal of Animal & Plant Sciences 24: 221−228.
Bertaccini, A. 2007. Phytoplasmas: Diversity, Taxonomy, and Epidemiology. Frontiers in Bioscience 12: 673–689.
Bertacini, A. & B. Duduk. 2009. Phytoplasm and Phytoplasm Diseases: A Review of Recent Research. Phytopathologia Mediterranea 48: 355−378.
Constable, F.E., K.S. Gibb, & R.H. Symons. 2003. Seasonal Distribution of Phytoplasm in Australian Grapevines. Plant Pathology 52: 267−276.
Davis, R.I., P. Kokoa, L.M. Jones, J. Mackie, J., F.E. Constable, B.C. Rodoni, T.G. Gunua, & J.B. Rossel. 2012. A New Wilt Diseases of Banana Plants Associated with Phytoplasms in Papua New Guinea (PNG). Australian Plant Disease Notes 7: 91−97.
Davis, R.I., J. Henderson, L.M. Jones, A.R. McTaggart, C. O’Dwyer, F. Tsatsia, C. Fanai, & Rossel, J.B. 2015. First Record of a Wilt Diseases of Banana Plants Associated with Phytoplasms in Solomon Islands. Australian Plant Disease Notes 10: 14.
Dellaporta, S.L., J. Wood, & J.B. Hicks. 1983. A Plant DNA Mini Preparation: Version II. Plant Molecular Biology Reporter 1: 19−21.
Deng, S. & C. Hiruki. 1991. Amplication of 16S rRNA genes from Culturable and Nonculturable Mollicutes. Journal of Microbiological Methods 14: 53−61.
Dickinson, M., M. Tuffen, & J. Hodgetts. 2013. The Phytoplasms: An Introduction, p. 1−14. In M. Dickinson & J. Hodgetts (eds.), Phytoplasm Methods and Protocols. Humana Press, New York (USA).
Duduk, B., S. Paltrinieri, I.M. Lee, & A. Bertaccini. 2013. Nested-PCR and RFLP Analysis Based on the 16S rRNA Gene, p. 159−172. In M. Dickinson & J. Hodgetts (eds.), Phytoplasm Methods and Protocols. Humana Press, New York (USA).
Dwimartina, F., T. Arwiyanto, & T. Joko. 2017. Potential of Endophytic and Rhizobacteria as an Effective Biocontrol for Ralstonia syzygii subsp. syzygii. Asian Journal of Plant Pathology 11: 191−198.
Gibb, K. & A. Padovan. 1994. A DNA Method that Allows Reliable PCR Amplification of MLO DNA from Difficult Plant Host Species. PCR Method & Application 4: 56−58.
Handiyanti, M., S. Subandiyah, & T. Joko. 2018. Deteksi Molekuler Burkholderia glumae, Penyebab Penyakit Hawar Malai Padi. Jurnal Perlindungan Tanaman Indonesia 22: 98−107.
Ismiyatuningsih, T. Joko, & S. Hartono. 2016. Survey and Detection of Pectobacterium atrosepticum in Major Potato-Growing Areas in Central Java Province, Indonesia. Ilmu Pertanian 1: 1−6.
Joko, T., S. Subandiyah, & S. Somowiyarjo. 2000. The Role of Extracellular Protein on the Pathogenicity of Xanthomonas campestris pv. citri. Jurnal Perlindungan Tanaman Indonesia 6: 32−38.
Joko, T., M. Umehara, T. Murata, H. Etoh, K. Izumori, & S. Tsuyumu. 2018. Hyperinduction of Pectate Lyase in Dickeya chrysanthemi EC16 by Plant- Derived Sugars. Journal of Plant Interactions 13: 141−150.
Kirkpatrick, B.C., C.D. Smart, S.L. Gardner, U. Ahrens, R. Ma’urer, B. Schneider, K.H.M. Lorenz, E. Seemuller, N.A. Harrison, S. Namba, & X. Daire. 1994. Phylogenetic Relationships of Plant Pathogenetic MLOs Established by 16/23S rDNA Spacer Sequences. International Organitation for Migration Letter 3: 228−229.
Kumar, S., G. Stecher, & K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Data Sets. Molecular Biology and Evolution 33: 1870−1874.
Lee, I.M., R.E. Davis, D.F. Gundersen, & Rindal. 2000. Phytoplasm, Phytopathogenic Mollicutes. Annual Review of Microbiology 54: 221−225.
Lou, B., X. Bai, Y. Bai, C. Deng, M.R. Chowdhury, C. Chen, & Y. Song. 2013. Phytoplasm in Grapefruit (Citrus paradisi) with Huanglongbing-Like Symptoms in China. Journal of Phytopathology 16: 387−395.
Maust, B.E., C.F. Espadas, M. Talavera, J.M. Aguilar, Santamaría, & C. Oropeza. 2003. Changes in Carbohydrate Metabolism in Coconut Palms Infected with the Lethal Yellowing Phytoplasm. Phytopathology 93: 976−981.
Mahfut, T. Joko, & B.S. Daryono. 2016. Molecular Characterization of Odontoglossum Ring Spot Virus (ORSV) in Java and Bali, Indonesia. Asian Journal of Plant Pathology 10: 9−14.
Musetti, R. 2010. Biochemical Changes in Plant Infected by Phytoplasm, p. 132–146. In P.G. Weintraub & P. Jones (eds.), Phytoplasms: Genomes, Plant Hostsand Vectors. CAB International, Wallingford (UK).
Nurjanah, N., T. Joko, & S. Subandiyah. 2017. Characterization of Pantoea ananatis Isolated from Garlic and Shallot. Jurnal Perlindungan Tanaman Indonesia 21: 120−126.
Prasetyo, A.E., K.H. Mutaqin, & Giyanto. 2017. Deteksi dan Identifikasi Phytoplasm yang Berasosiasi dengan Penyakit Layu Kelapa di Pulau Derawan Kalimantan Timur. Jurnal Fitopatologi Indonesia 13: 89−97.
Suyanti & A. Supriyadi. 2008. Pisang, Budidaya, Pengolahan, dan Prospek Pasar. Penebar Swadaya, Depok. 132 p.
Semangun, H. 2007. Penyakit-Penyakit Tanaman Hortikultura di Indonesia. Gadjah Mada University Press. Yogyakarta. 845 p.
Warokka, J.S., P. Jones, & M.J. Dickson. 2006. Detection of Phytoplasm Associated with Kalimantan Wilt Disease of Coconut by the Polymerase Chain Reaction. Jurnal Penelitian Tanaman Industri 12: 154–160.
Wibowo A., T. Joko, S. Subandiyah, I. Mariska, Y. Supriyati, Y. Suryadi, & I. Roostika. 2010. Peningkatan Ketahanan Tanaman Pisang Kepok Kuning terhadap Penyakit Darah melalui Variasi Somaklonal dan Simbiosis Endofitik. Jurnal Perlindungan Tanaman Indonesia 16: 15–21.
Windari, U., T. Joko, & S. Subandiyah. 2015. Deteksi Penyakit Bacterial Fruit Blotch pada Melon Menggunakan ELISA. Jurnal Perlindungan Tanaman Indonesia 19: 1−5.
Wei, W., R.E. Davis, I.M. Lee, & Y. Zhao. 2007. Computer-simulated RFLP Analysis of 16S rRNA Genes: Identification of Ten New Phytoplasm Groups. International Journal of Systematic and Evolutionary Microbiology 57: 1855–1867.
DOI: https://doi.org/10.22146/jpti.38320
Article Metrics
Abstract views : 2650
| 
views : 2126
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics