Pemendekan Waktu ELISA dalam Deteksi TMV

https://doi.org/10.22146/jpti.12891

Susamto Somowiyarjo(1*), Y. B. Sumardiyono(2), Purwati Widiyati(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


In order to support the program for the management of viral diseases on garlic, a rapid diagnosis procedure was developed by shortening the incubation period of the indirect ELISA. No significant differences of ELISA absorbance were observed when the antigen was incubated for 4 h, 2 h, and 30 min at 37ºC. The incubation period for the antibody and conjugate could he reduced from 4 h at 37ºC or 18 h at 4ºC to 30 min al 37ºC. The shortest period of incubation for the assay could be obtained when each incubation time for the antigen, antibody and conjugate was 30 min. The detection limit for the shortened ELISA was 10^-4 for the virus in crude extracts and 0,5 μg/ml for the purified preparation.


Keywords


TMV; detection; ELISA

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References

Bos, L. 1983. Introduction to Plant Virology. (Pengantar Virologi Tumbuhan, alih bahasa Triharso ). Gadjah Mada University Press. Yogyakarta. 226 hal.

Butler, J.E. 1988. The Amplified ELISA (a-ELISA): lmmunochemistry and Applications. In Kemeny, D. M. and S.J. Challacombe (Eds.).: ELISA and Other Solid Phase Immunoassay. Theoretical and Practical Aspects. John Wiley and Sons. New York. p. 107-133.

Challacombe, S.J. 1988. Application of ELISA to Microbiology. In Kemeny, D.M. and S.J. Challacombe (Eds.): ELISA and Other Solid Phase Immunoassay. Theoretical and Practical Aspects. John Wiley and Sons. p. 319-342.

Clark, M.F. 1981. lmmunosorbent Assays in Plant Pathology. Ann. Rev. Phytopatholology, 19: 83-106.

Cushing, J.E. and D.H. Campbell. 1957. Principles of Immunology. McGraw-Hill Book Company, Inc. Kew York. 344 p.

Hartono, S. 1996. Deteksi Virus Bawang Putih dengan ELISA. Tesis S-2. Program Pasca Sarjana UGM. Yogyakarta.

Kemeny, D.M. and S. Chantler. 1988. An Introduction to ELISA. In Kemeny, D.M. and S.J. Challacombe (Eds.).: ELISA and Other Solid Phase Immunoassay. Theoretical and Practical Aspects. John Wiley and Sons. New York. p. 1-29.

Kemeny, D.M. and S.J. Challacombe. 1988. Microtitre Plates Other Solid Phase Supports. In Kemeny, D.M. and S. J. Challacombe (Eds.).: ELISA and Other Solid Phase Immunoassay. Theoretical and Practical Aspects. John Wiley and Sons.p.31-55.

Koenig. R. 1981. Indirect ELISA Methods for The Broad Specificity Detection of Plant Viruses. J. Gen. Virol. 55 : 53-62.

Lommel, S.A., A.H. McCain, and T.J. Morris. 1982. Evaluation of Indirect Enzyme-Linked Immunosorbent Assay for The Detection of Plant Viruses. Phytopathology. 12: 1018-1022.

Martin, R.R. 1985. Recent Advances in Virus Detection. Hortscience. 20(5) : 837-845.

Van Regenmortel, M.H.V. and J. Burckard. 1980. Detection of a Wide Spectrum of Tobacco Mosaic Virus Strains by Indirect Enzyme-Linked lmmunosorbent assays (ELISA). Virology 106 : 327-334.



DOI: https://doi.org/10.22146/jpti.12891

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Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

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