Analisis Aktivitas Enzim Peroksidase dan Kandungan Asam Salisilat dalam Tanaman Cabai Merah yang Diinduksi Ketahanannya terhadap Cucumber Mosaic Virus (CMV) oleh Ekstrak Daun Bunga Pukul Empat (Mirabilis jalapa)

https://doi.org/10.22146/jpti.12104

Hersanti Hersanti(1*)

(1) Fakultas Pertanian, Universitas Padjadjaran
(*) Corresponding Author

Abstract


Leaf crude extract of Mirabilis jalapa is an inducer agent of systemic resistance of red chilli to CMV. This experiment was objected to study the activity of peroxidase enzyme and the concentration of salicylic acid in the induced resistant plant to CMV by M. jalapa leaf crude extract. The results showed that the induced resistant plant to CMV by leaf crude extract of M. jalapa had a low CMV disease intensity, low content of virus, increased the activity of peroxidase enzyme 2 - 10 times; increased the salicylic acid 1.6 - 5 times. There were a significant interaction effect between disease intensity of CMV and salicylic acid content (r = 0.94), middle interaction effect between disease intensity of CMV and salicylic acid content (r = 0.46), low interaction effect between the virus content and disease intensity of CMV (r = 0.32), and salicylic acid and activity of peroxidase enzyme (r = 0.39); and no interaction effect between virus content and salicylic acid content (r = 0.05), and between virus content and activity of peroxidase enzyme ( r = 0.12 ).

Keywords


M. jalapa; CMV; peroxidase enzyme; salicylic acid; Systemic Induce Resistance

Full Text:

PDF


References

Djarwanto, & P. Subagyo. 1993. Statistik Induktif. Cetakan ke-4. BPE-Yogyakarta. 327 hal.

Dolores, L.M. 1996. Management of Pepper Viruses. Pp.: 334-342. In AVNET-II Final Workshop Proceedings. AVDRC. Tainan, Taiwan.

Duriat, A.S., W. Setiawati & R. Sutarya. 1991. Bioekologi penyakit krupuk pada tanarnan cabai. I. Studi penularan penyakit krupuk pada cabai. hal: 32-34. Dalam Pengendalian Terpadu Hama dan Penyakit kentang, kubis, cabai dan bawang merah Laporan PHT ARM 90/91. Balihort Lembang.

Gupta, S.K., P.P. Gupta, T.P. Yadava, & C.D. Kaushik. 1990. Metabolic changes in mustard due to Alternaria leaf blight. Indian Phytopathol. 43(1): 64-69.

Hersanti, C. Nasahi, & T. Sunarto. 2003. Pengujian beberapa ekstrak tumbuhan sebagai agen penginduksi ketahanan tanaman cabai merah terhadap Cucumber Mosaic Virus (CMV). J. Agrik. 14 (3): 160-165.

Hersanti. 2003. Pengujian potensi ekstrak 37 Species tumbuhan sebagai agen penginduksi ketahanan sistemik tanaman cabai merah terhadap Cucumber Mosaic Virus. J. Fitopat. Ind. 7(2) : 54-58.

Hopkins, W.G 1999. Introduction to Plant Physiology. 2nd edition. Academy Press.New York.

Kessmann, H., T. Staub, C. Hofmann, T. Maetzke, J. Herzog, E. Ward, S. Uknes & J. Ryals. 1994. Induction of systemic acquired disese resistance in plants by chemicals. Annu. Rev. Phytopathol. 32 : 439-459.

Kuc, J. 1987. Plant Immunization and its Applicability for Disease Control. Pp. 225-272. In I. Chet (Ed.). Innovative Approaches to Plant Disease Control. John Wiley and Sons, New York.

Loebenstein, G. & N.N. Lindsey. 1961. Peroxidase activity in virus infected potatoes. Phytopathology 51: 533-537.

Louws, F.J., K.H. Mary, F.K. John, & T.S. Cristine. 1996. Impact of reduced fungicide and tillage on blight, fruit root and yield processing tomatoes. Plant Dis. 80: 1251-1256.

Mafrukhin, M., D.S. Utami,& Kustatinah. 2001. Pemanfaatan agen antiviral Mirabilis jalapa untuk menekan penyakit karena mosaik virus pada tanaman cabai merah. Buku panduan KSN PF/ 2001.

Martinez, C., J.C. Baccou, E. Bresson, Y. Baissac, J.F. Daniel, A. Jalloul, J.L. Montilet, J.P. Geiger, K. Assigbetse, & M. Nicole. 2000. Salicylic acid mediated by the oxidative burst is a key molecule in local and systemic response of catton challenged by a virulent race of Xanthomonas campestris pv. malvacearum. Plant Physiol. 22 : 756 - 766.

Mc.Kee,T & J. Mc. Kee. 1999. Biochemistry: An Introduction.Second ed. Mc.Graw-Hill.New York. Molina, A., M., D. Hunt & J. A. Ryals. 1998. Impaired fungicide activity in plants blocked in disease resistance signal transduction. Plant Cell 10: 1903-1914.

Murphy, A.M., A. Gilliand, C.E. Wong, J. West, D.P. Singh & J.P. Carr. 2001. Signal transduction in resistance to plant viruses. Euro.J. Plant Pathol. 107 :121-128.

Naylor,M., A.M. Murphy, J.O. Berry, & J.P. Carr. 1998. Salicylic acid can induce resistance to plant virus movement. Molecular Plant Microbe lnterac. 11 :860-866.

Nicks, R.E., P.R. Ellis, & J.E. Palevliet. 1993. Resistance to Parasites. Pp. : 442-447. In M.D. Hayward, N.O. Bosemark, and I. Romagosa (Eds.) Plant Breeding Principles and Prospects. Chapman and Hall. London.

Sari, C.N., I, R. Suseno, Sudarsono, & M. Sinaga. 1997. Reaksi sepuluh galur cabai terhadap infeksi isolat CMV dan PVY asal Indonesia. Prosiding Kongres Nasional dan Seminar llmiah PFI. Palembang 27-29 Oktober 1997. Hal: 116-119.

Somowiyarjo, S., Y.B. Sumardiyono, & Shofar Martono. 2001. Inaktivasi CMV dengan ekstrak Mirabilis jalapa. Presiding Kongres Nasional XVI dan Seminar llmiah, PFI. Bogor. 22-24 Agustus 2001.

Tenhaken, R. & C. Rubel. 1997. Salicylic acid needed in hypersensitif cell death in soybean but does not act a catalase inhibitor. Plant Physiol. 115: 291-298.

van Loon, L.C., W.S. Pierpoint, Th. Boller, & V. Conejero. 1994. Recommendations for naming plant pathogenesis-related proteins. Plant Molecular Biology Report. 12 : 245-264.

Verma, H.N., S. Srivastava, Varsha, & D. Kumar. 1996. Induction of systemic resistance in plants against Viruses by a basic protein from Clerodendrum aculeatum leaves. Phytopathology 86 : 485-492.

Vivanco, JM, M. Querci & LF. Salazar. 1999. Antiviral and antiviroid activity of MAP containing extract from Mirabilis jalapa roots. Piant Disease 83 : 1116 - 1121.

Wei, G, J.W. Kloepper, & S. Tuzun. 1996. Induced systemic resistance to cucumber diseases and increased plant growth by plant growth-promoting rhizobacteria under field conditions. Phytopathology 86: 221-224.

Yalpani, N., V. Shulaev, & I. Raskin. 1993. Endogenous salicylic acid levels correlated with accumulation of pathogenesis-related proteins and virus resistance in tobacco. Phytopathology. 83: 702-708.

Zhou, B.W., S.Y. Liu, D.Y. Chen, Q. Yu, J. Yang, & C. Wang. 1992. Peroxidase in relation to varietas resistance to virus disease in rapeseed (Brassica napus). (Abstract). Oil Crops of China 2 : 52-54.



DOI: https://doi.org/10.22146/jpti.12104

Article Metrics

Abstract views : 6940 | views : 9125

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2005 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics