Pemurnian dan Deteksi Serologi Patchouli Mottle Virus pada Tanaman Nilam

https://doi.org/10.22146/jpti.12044

Sedyo Hartono(1*), Siti Subandiyah(2), Aminatun Munawarti(3), Retno Mastuti(4), Serafinah Indriani(5)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Jurusan Biologi Fakultas MIPA Universitas Brawijaya
(4) Jurusan Biologi Fakultas MIPA Universitas Brawijaya
(5) Jurusan Biologi Fakultas MIPA Universitas Brawijaya
(*) Corresponding Author

Abstract


Patchouli mottle virus (PatMoV) is the most severe disease pathogen and causes substantial losses in many patchouli-producing regions in Indonesia. Serological detection tool  for the disease was developed in this  research. Virus isolation was conducted on Chenopodium amaraticolor resulted on  the homogenous local lesions 6 days after  inoculation. Virus  purification was obtained from 200g inoculated leaves resulted on 2 ml virus solution with the concentration of 1 mg/ml. Polyclonal antibodies were produced on rabbits. Harvested antiserum was used for further virus detection by Indirect-Enzyme linked Immunosorbent assay (I-ELISA) and dot-immunobanding assay (DIBA) techniques. The antibodies were  positively  reacted with purified  viruses, infected field collection of patchouli, and  inoculated C. amaranticolor. On the other hand un-inoculated C. amaranticolor samples and healthy patchouli generated from tissue cultures gave negative reaction with the antibodies. This is the first report of cheap practical antibody production for PatMoV detection in Indonesia.

Keywords


patchouli; polyclonal antibody; Patchouli mottle virus; ELISA; DIBA

Full Text:

PDF


References

Asman, A., Natsir, N., Nurawan, A., & Sitepu, D. 1993. Penelitian Penyakit Nilam. Prosiding Kong. Nas. PFI XII. Yogyakarta 6-8 September. p.903-911.

Banttari, E.E. & P.H. Goodwin. 1985. Detection of Potato Virus S, X, and Y by Enzyme-linked Immunosorbent assay on Nitrocellulose Membrane (Dot-ELISA). Plant Diseases 69:202-205.

Hibi, T. & Y. Saito. 1985. A Dot-Immunobinding Assay for the Detection of Tobacco mosaic virus in Infected Tissues. J. Gen. Viral. 66:1191-1194.

Hartono, S. 1992. Sifat-sifat virus penyebab penyakit mosaik kuning pada nilam Aceh (Pogostemon cablin). Tesis. Fakultas Pertanian UGM. Yogyakarta. (Tidak dipublikasikan).

Hull, R. 2002. Mathews Plant Virology. Academic Press. San Diego, California, USA. Koenig, R. 1981. Indirect ELISA methoda for the broad specificity detection of plant viruses. J. Gen. Virol. 55:53-62.

Kusnanta, A. 2005. Identifikasi dan Pengendalian Penyakit Karat Palsu pada Tanaman Nilam (Pogostemon cablin) dengan Fungisida. Tesis-S2. Sekolah Pasca Sarjana, Universitas Gadjah Mada. Yogyakarta. 36p. (tidak diterbitkan)

Mansur, M. & Tasma, I.M. 1987. Plasma nutfah tanaman nilam. Dalam: Penelitian tanaman rempah dan obat. Edisi Khusus III(l):59-63. Balai Penelitian Rempah dan Obat. Bogor.

Natsuaki, K.T., Tomaru, K., Cheng, C.S., & Tanaka, K. 1989. Two viruses isolated from patchouli plants. NODAI Res. Inst. Tokyo University of Agriculture. Tokyo. Japan.

Natsuaki, K.T., Tomaru, K., Ushiku, Y., Sugimura, Y., Natsuaki, T., Okuda, S. & Teranaka, M. 1994. Characterization of two viruses isolated from patchouli in Japan. Plant Diseases 78: 1094-1097.

Nur Aeny, T. 1985. Inventarisasi penyakit nilam (Pogostemon cablin Benth.) di perkebunan Cireundeu, Kec. Nagrak, Kab. Sukabumi, Jawa Barat. 38 p.

Rukmana, R. 2004. Nilam: Prospek Agribisnis dan Teknik Budidaya. Penerbit Kanisius. Yogyakarta.

Sumardiyono, Y.B., Sri Sulandari, & Sedyo Hartono. 1993. Penyakit mosaik pada nilam (Pogostemon cablin). Prosiding Kong. Nas. PFI XII Yogyakarta 6-8 September. p 912-916.

Thomas, J.E., Geering, A.D.W., Gambley, C.F., Kessling, A.F., & White, M. 1997. Purification, properties, and diagnosis of banana bract mosaic potyvirus and its distinction from abaca mosaic potyvirus. Phytopathology 87:698-705.

Tjiptadi, GH.B. 1985. Pengembangan usaha minyak atsiri. Balai Pengembangan Khemurgi dan Aneka Industri. BBPPIHP. Bogor.

Yuliani, A. 1991. Inventarisasi penyakit-penyakit tanaman nilam di daerah tingkat II Banyumas, Jawa Tengah. Tesis. Fakultas Pertanian UGM. Yogyakarta. (Tidak dipublikasikan).



DOI: https://doi.org/10.22146/jpti.12044

Article Metrics

Abstract views : 1448 | views : 2074

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2006 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics