Characterization of A Baculovirus of Spodoptera litura (Lepidoptera : Noctuidae) Isolated from Yogyakarta

https://doi.org/10.22146/jpti.11813

Arman Wijonarko(1*), Sedyo Hartono(2), Tri Harjaka(3), Nuri Yusmarlita(4)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(4) Graduate School of Agriculture Tokyo University of Agriculture and Technology
(*) Corresponding Author

Abstract


A Baculovirus has been isolated from cadaver of larvae of Spodoptera litura, a Noctuidae of agricultural pest, importance due to its wide-range hosts and the damage to their respective host. Phase contrast light microscopy observation from infected larvae showed that the fat body, hemocyte cells, and cells surrounding the trachea or tracheolus were the most tissue invaded by polyhedra. Transmission electron microscopy analysis of the occlusion body purified from diseased larva showed that the baculovirus envelope containing multiple nucleocapsid. Digestion of viral DNA with three restriction enzymes showed that the genome pattern of baculovirus isolated from Bantul were close to SpliNPV isolated from Japan and those of Spodoptera littoralis and quite distinct from those isolated from Southeast Asia region. Bioassay test performed on first to fifth instar larvae showed that the virus effectively control the young larvae, but showed some level of resistance against older larvae of Spodoptera litura.

Keywords


characterization; baculovirus; Spodoptera litura; Yogyakarta

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References

Briese, D. T. 1986. Insect resistance to baculovirus. In : The Biology of Baculoviruses, Vol.2. R.R. Granados and B.A. Federici (eds.). CRC Press, Boca Raton, Florida, pp. 237-263

Finney, D.J. 1978. Statistical Method in Biological Assay 3rd ed. Charles Griffin and Co. London. 508 pp. Federici, B. A. 1997. Baculoviruses pathogenesis. In: The Baculoviruse. Miller, L.K. (ed.). Plenum. New York, pp. 33-59

Fuxa, J.R. & Y Tanada. 1987. Epizootiology of Insect Diseases. John Wiley and Sons,New York. 555 pp.

Hemiou, E.A, Olezewski, J.A, O'Reilly, D.R., & Cory, J.S. 2003. The genome sequence and evolution of baculoviruses. Ann. Rev. Entomol. 48, 211-234

Huber, L. & P.R. Hughes.1984. Quantitative bioassay in insect pathology. Bull. Entomol. Soc. Am. 30:31-34

Hughes, P.R. & H.A. Wood. 1981. A sychronous peroral technique for the bioassay of insect viruses. J Invertebr. Pathol. 37:154-159

Ishii, T., Nakai, M., Okuno, S., Takatsuka, J., & Kunimi, Y. 2003. Characterization of Adoxophyes honmai single-nucleocapsid nucleopolyhedrovirus: morphology, structure, and effect on larvae. J. Invertebr. Pathol. 83: 206-214

Kunirni, Y 1998. A world survey of virus control of insect pest; Japan. In: Hunter, F.R., Entwistle, P.F., Evans, H.F., Crook, N.E. (Eds.). Insect Viruses and Pest Management. John Wiley & Sons. West Sussex, pp. 269-279

Kunimi, Y. & J.R. Fuxa. 1996. Volume ingested by four species of noctuids with reference to peroral droplet bioassay of baculoviruses. J Invertebr. Pathol. 68:310-311

Luckow, VA. 1995. Protein production and processing from baculovirus expression vectors. In: Baculovirus Expression System and Biopesticides. Wiley-Liss, pp. 51-90

Moscardi, F. 1999. Assesment of the application of baculovirus for control lepidoptera. Ann. Rev. Entomol. 44: 257- 289

Sakuma, M. 1998. Probit analysis of preference data. Appl. Entomol. Zoo!. 33: 339-347

Sambrook, J., Fritsch, E.F., & Maniats, T. 1989. Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor.

Smits, P.H. 1987. Nuclear polyhedrosis virus as biological control agent of Spodoptera exigua. PhD thesis. Wageningen Agricultural University. 127 pp 72.

Volkman, L.E., Blissard, G W., Friensen, P.D., Posse, R.D., & Theilmann, D.A. 1995. Baculoviridae. In: Murphy, F., Fauquet, C.M., Bishop, D.H.L., Jarvis, A. W., Martelly, G, Mayo, E., Summers, M.D. (Eds.), Virus Taxonomy: Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses. Arch. Virol., Suppl. 10. pp. 104-113



DOI: https://doi.org/10.22146/jpti.11813

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