Survey on the Occurrence of Viruses Infecting Cucurbits in Yogyakarta and Central Java
Budi Setiadi Daryono(1*), Keiko T. Natsuaki(2)
(1) Fakultas Biologi Universitas Gadjah Mada
(2) Graduate School of Agriculture Tokyo University of Agriculture
(*) Corresponding Author
Abstract
Cucurbits are grown throughout the Java Island as dry season crops. Plants having mosaic, mottling, chlorosis and leaf distortion symptoms were frequently found in most of the cucurbit fields during the survey which conducted in Central Java including Sleman, Kulon Progo, and Klaten during July–September 2000 and 2001. Using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA); Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV) and Kyuri green mottle mosaic virus (KGMMV) were found infecting cucurbits. CMV was widespread, infecting 48.9% of the samples tested followed by CGMMV (12.8%) and KGMMV (6.4%), while others samples (31.9%) were not tested, double infections were common with 8.5 % of the samples being infected with two viruses (CGMMV and KGMMV) and 34% with three viruses (CMV, CGMMV, and KGMMV). Severe mosaic and mottle symptoms were associated most often with single infection of CGMMV and KGMMV respectively. In addition, these are the first detections of CGMMV and KGMMV infecting cucurbit plants in Indonesia.
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Ainsworth, G.C. 1935. Mosaic Disease of Cucumber. Annals of Applied Biology 22: 55–67.
Anonim. 2003. Buku Statistik Indonesia. Badan Pusat Statistik Indonesia, Jakarta. 21 p.
Antignus, Y., Y.Wang,M. Pearlsman, O. Lachman, N. Lavi, & A. Gal-On. 2001. Biological and Molecular Characterization of A New Cucurbitinfecting Tobamovirus. Phytopatology 91: 565–571.
Clark, M.F. & A.N. Adams. 1977. Characteristics of the Microplate Method of Enzyme-linked Immunosorbent Assay for the Detection of Plant Viruses. Journal of General Virology 34: 475–483.
Daryono, B.S., Somowiyarjo, S., & K.T. Natsuaki. 2005. Biological andMolecular Characterization of Melon-infecting Kyuri Green Mottle Mosaic Virus in Indonesia. Journal of Phytopathology 153: 588–595.
Daryono, B.S., S. Somowiyarjo, & K.T. Natsuaki. 2006. Biological Characterization and Complete Nucleotide Sequence of Coat Protein of Kyuri Green Mottle Mosaic Virus Isolated from Angled Loofah in Indonesia. Journal of Agricultural Science, Tokyo University of Agriculture 51: 45–52.
Davis, R.F. & M.K. Mizuki. 1987. Detection of Cucurbit Viruses in New Jersey. Plant Disease 71: 40–44.
Grafton-Cardwell, E.E., T.M. Perring, R.F. Smith, J. Valencia, & C.A. Farrar. 1996.
Occurrence of Mosaic Viruses in Melons in the Central Valley of California. Plant Disease 80: 1092–1097.
Hartana, I. 1987. Penyakit-Penyakit Utama pada Tanaman Tembakau di Jawa Timur dan Usaha Pengendaliannya. In I.R. Sastrahidayat, M.C. Machfud, & S. Djauhari (eds.), Prosiding Kongres Nasional IX PFI, Surabaya. Perhimpunan Fitopatologi Indonesia, Surabaya.
Inoue, T., N. Inoue, M. Asatani, & K. Mitsuhata. 1967. Studies on Cucumber Green Mottle Mosaic Virus in Japan (in Japanese). Nogaku Kenkyu 51: 175–186.
Kitani, K.,A. Kiso, &Y. Shigematsu. 1970. Studies on A New Virus Disease of Cucumber (Cucumber sativus L. var. F1 Kurume-Oitai-H type) Discovered in Yodo (in Japanese). Proceeding of the Association for Plant Protection of Shikoku 5: 59–66.
Komuro Y., H. Tochihara, R. Fukatsu, Y. Nagai, & S. Yoneyama. 1968. Cucumber Green Mottle Mosaic Virus on Watermelon in Chiba and Ibaraki Prefectures (in Japanese). Annals of the Phytopathology Society of Japan 34: 377.
Lee, S.H., Y.G. Lee, J.W. Park, J.U. Cheon, K.W. Lee, & Y.C. Choi. 2000. Nucleotide Sequence of Coat Protein Gene of Kyuri Green Mottle Mosaic Virus (KGMMV) Isolated from Zucchini (Cucurbita pepo) in Korea. Plant Pathology Journal 16: 118–124.
Luis-Artega,M., J.M.Alvarez, J.L.Alonso-Prados, J.J. Bernal, F. Garcia-Arenal, A. Lavina, A. Batle, & E.Moriones. 1998. Occurrence, Distribution, and Relative Incidence of Mosaic Viruses Infecting Field-grown Melon in Sapin. Plant Disease 82: 979–982.
Palukaitis, P., M.J. Roossinck, R.G. Dietzgen, & R.I.B. Francki. 1992. Cucumber Mosaic Virus. Advance Virus Research 41: 281–348.
Ryu, K.H. 2004. Analysis of Cucurbit-infecting Tobamoviruses and Plant Virus Sequencing in Korea, p. 31–42. In K. Tomaru (ed.), PSJ Plant Virus Disease Workshop Report. No. 7. Sammons, B., O.W. Barnett, R.F. Davis, & M.K. Mizuki. 1989. A Survey of Viruses Infecting Yellow Summer Squash in South Carolina. Plant Disease 73: 401–404.
Semangun, H. 1989. Penyakit-Penyakit Tanaman Hortikultura di Indonesia Gadjah Mada University Press, Yogyakarta. 124 p.
Somowiyarjo, S., N. Sako, & K. Tomaru. 1993. The Use of Dot Immunobinding Assay for Detecting Cucurbit Viruses in Yogyakarta, p. 3–11. In K. Tomaru & K.T. Natsuaki (eds.), Production of Virus-free Tropical Crops. NODAI Center for International Programs. Tokyo University of Agriculture, Tokyo.
Suastika, G., K. Tomaru, J. Kurihara, & K.T. Natsuaki. 1995. Characteristics of Two Isolates of Cucumber Mosaic Virus Obtained from Banana Plants in Indonesia (abstract in Japanese). Annals of the Phytopathology Society of Japan 61: 272.
Sulandari, S. 1990. Deteksi Cucumber mosaic virus dengan Metode Serologi pada Tanaman Hias dan Sayuran di DIY. Laporan Penelitian. Fakultas Pertanian UGM, Yogyakarta.
Suseno, H.R. & J.R. Lumanau. 1972. Occurrence of Cucumber Mosaic Virus on Tobacco Plants in Indonesia. South East Asian Regional Symposium on Plant Diseases in the Tropics, Yogyakarta.
Tan, S.H., M. Nishiguchi, M. Murata, & F. Motoyoshi. 2000. The Genome Structure of Kyuri Green Mottle Mosaic Tobamovirus and its Comparison with that of Cucumber Green Mottle Mosaic Tobamovirus. Archives of Virology 145: 1067–1079.
Ugaki, M., M. Tomiyama, T. Kakutani, S. Hidaka, T. Kiguchi, R. Nagata, T. Sato, F. Motoyoshi, & M. Nishiguchi. 1991. The Complete Nucleotide Sequence of Cucumber GreenMottleMosaic Virus (SH Strain) Genomic RNA. Journal of General Virology 72: 1487–1495.
Vasudeva, R.S., S.P. Raychaudhuri, & J. Singh. 1949. A New Strain of Cucumis Virus 2. Indian Phytopathology 2: 180–185.
DOI: https://doi.org/10.22146/jpti.11769
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