Deteksi Keragaman Virus Tungro dari Beberapa Daerah Endemis di Indonesia dengan Teknik PCR-RFLP

https://doi.org/10.22146/jpti.11763

R. Heru Praptana(1*), Y. B. Sumardiyono(2), Sedyo Hartono(3), I Nyoman Widiarta(4), Muhammad Muhsin(5)

(1) Sekolah Pascasarjana Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(4) Pusat Penelitian dan Pengembangan Tanaman Pangan
(5) Balai Besar Biogen
(*) Corresponding Author

Abstract


Tungro is one of rice disease caused by two different viruses (rice tungro virus=RTV) i.e. Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV) that are transmitted only by green leafhopper. Tungro had become a serious problem in several rice productions centre in Indonesia. Various components of management effort have been applied but they were inefficient in preventing the tungro disease development. Resistance variety is the most efficient component to tungro disease management. Complexity interactions of tungro disease components are mayor constraint in tungro disease management. Detection of molecular variability in rice tungro virus from several endemic areas in Indonesia were conducted by using PCR-RFLP technique. Existence of RTBV and RTSV in the infected plants collected from several endemic areas were successfully detected by PCR. The RFLP analysis with restriction enzymes BstYI and HindIII showed that there were significant difference among the RTSV originated from Java, Bali and Sulawesi.


Keywords


endemic areas and molecular variability in RTV; RTBV; RTSV; tungro; tungro disease

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References

Azzam, O. & T.C.B. Chancellor. 2002. The Biology, Epidemiology, and Management of Rice Tungro Disease in Asia. Plant Disease 86: 88–100.

Baehaki, S.E. & H. Suharto. 1985. Penyakit Tungro. Makalah Temu Lapang Pengendalian Penyakit Tungro di Banyumas, 18–19 September 1985. 48 p.

Cabauatan, P.Q., M. Arboleda, & O. Azzam. 1998. Differentiation of Rice Tungro Bacilliform Virus Strains by Restriction Analysis and DNA Hybridization. Journal of Virological Methods 76: 121–126.

Cabauatan, P.Q., U. Melcher, K. Ishikawa, T. Omura, H. Hibino, H. Koganezawa, & O.Azzam. 1999. Sequence Changes in Six of Rice Tungro Bacilliform Virus and their Phylogenetic Relationships. Journal of General Virology 80: 2229–2237.

Choi, R.I. 2004. Current Status of Rice Tungro Disease Research and Future Program. p. 3–14. In A. Hasanuddin, I.N. Widiarta & Sunihardi (eds.), Strategi Pengendalian Penyakit Tungro: Status dan Program. Prosiding Seminar Nasional Status Program Penelitian Tungro Mendukung Keberlanjutan Produksi Padi Nasional. Makassar, 7–8 September 2004.

Dahal, G., H. Hibino, & R. C. Saxena. 1990. Association of Leafhopper Feeding Behavior with Transmission of Rice Tungro to Susceptible and Resistant Rice Cultivars. Phytopathology 80: 371–377.

Deng, Z.N., A. Gentile, E. Nicolosi, E. Domina, A. Vardi, & E. Tribulato. 1995. Identification on in vivo and in vitro Lemon Mutants by RAPD Markers. Journal of Horticultural Science 70: 117–125.

Fan, Z., Dahal, G., Dasgupta, I., Hay, J., & Hull, R. 1996. Variation in the Genome of Rice Tungro Bacilliform Virus: Molecular Characterization of Six Isolates. Journal of General Virology 77: 847–854.

Gurr, S.J., M.J. McPherson, & D.J. Bowles. 1992. Molecular Plant Pathology. Vol. I. A Practical Approach. Oxford University Press, New York. 213 p.

Herzog, E., O. Guerra-Peraza, & T. Hohn. 2000. The Rice Tungro BacilliformVirus Gene II Product Interacts with the Coat Protein Domain of the Viral Gene III Polyprotein. Journal of Virology 74: 2073–2083.

Hibino, H., N. Saleh, & M. Roechan. 1977. Transmission of Two Kinds of Rice Tungro Associated Viruses by Insect Vectors. Phytopathology 69: 1266–1268.

Hibino, H. & R.C. Cabunagan. 1986. Rice Tungro Associated Viruses and their Relation to Host Plants and Vector Leafhopper. Tropical Agricultural Research Series 19: 173–182.

Innis, M.A. & D.H. Gelfald. 1990. Optimization of PCRs. p. 3–12. In Innis, M.A., D.H. Gelfald, J.J. Sninsky, & T.J. White (eds.), PCR Protocols- A Guide to Methods and Applications. Academic Press, San Diego, Inc, USA.

Isogai, M., P.Q. Cabauatan, C. Masuta, I. Uyeda, & O.Azzam. 2000. Complete Nucleotide Sequence of Rice Tungro Spherical Virus Genome of the Highly Virulent Strain Vt6. Virus Genes 20: 79–85.

Joshi, R., V. Kumar, & I. Dasgupta. 2003. Detection of Molecular Variability in Rice Tungro Bacilliform Viruses from India Using Polymerase Chain Reaction-/restriction Fragment Length Polymorphism. Journal of Virological Methods 109: 89–93.

Periasamy, M., F.R. Niazi, & V.G. Malathi. 2006. Multiplex RT-PCR, A Novel Technique for the Simultaneous Detection of the DNA and RNA Viruses Causing Rice Tungro Disease. Journal of Virological Methods 134: 230–236.

Praptana, R.H. & M. Yasin. 2008. Peranan Bioteknologi dalam Pengelolaan Penyakit Tungro. Iptek Tanaman Pangan 3: 98–111.

Raga, I.N., W. Murdita, M.P.L. Tri, S.W. Edi, & Oman. 2004. Sistem Surveillance Antisipasi Ledakan Penyakit Tungro di Indonesia. p. 49–59. In A. Hasanuddin, I.N. Widiarta, & Sunihardi (eds.), Strategi Pengendalian Penyakit Tungro: Status dan Program, Prosiding Seminar Nasional Status Program Penelitian Tungro Mendukung Keberlanjutan Produksi Padi Nasional. Makassar, 7–8 September 2004.

Siwi, S.S. & Y. Suzuki. 1991. The Green Leafhopper (Nephotettix spp.): Vector of Rice Tungro Virus Disease in SoutheastAsia, Particularly in Indonesia and its Management. Indonesian Agricultural Research and Development Journal 13: 8–15.

Soetarto,A., Jasis, S.W.G. Subroto,M. Siswanto, & E. Sudiyanto. 2001. Sistem Peramalan dan Pengendalian OPT dalam Mendukung Sistem Produksi Padi Berkelanjutan. p. 35–48. In I. Las, Suparyono, A.A. Daradjat, H. Pane, U.S. Nugraha, H.M. Toha, A. Tyasdjaya, & O.S. Lesmana (eds.), Prosiding Lokakarya Padi: Implementasi Kebijakan Strategi untuk Peningkatan Produksi Padi Berwawasan Agribisnis dan Lingkungan. Puslitbang Tanaman Pangan Bogor.

Sumardiyono, Y.B., S. Hartono, & I. Suswanto. 2004. Interaksi RTV dengan Wereng Hijau dan Daur Penyakit Tungro pada Padi. p. 37–47. In A. Hasanuddin, I.N. Widiarta, & Sunihardi (eds.), Strategi Pengendalian Penyakit Tungro: Status dan Program, Prosiding Seminar Nasional Status Program Penelitian Tungro Mendukung Keberlanjutan Produksi Padi Nasional. Makassar, 7–8 September 2004.

Suranto. 2004. Pengelolaan Virus Tungro Melalui Pendekatan Bioteknologi. Status dan Program Penelitian Pengendalian Terpadu Penyakit Tungro. p. 15–25. In A. Hasanuddin, I.N. Widiarta, & Sunihardi (eds.), Strategi Pengendalian Penyakit Tungro: Status dan Program, Prosiding Seminar Nasional Status Program Penelitian Tungro Mendukung Keberlanjutan Produksi Padi Nasional. Makassar, 7–8 September 2004.

Suzuki, Y., I.G.N. Astika, I.K.R. Widrawan, I.G.N. Gede, I.N. Raga, & Soeroto. 1992. Rice Tungro Disease Transmitted by Green Leafhoppers: its Epidemiology and Forecasting. JARQ 26: 98–104.

Takahashi, Y., F.R. Tiongco, P.Q. Cabauatan, H. Koganezawa, H. Hibino, & T. Omura. 1993. Detection Rice Tungro Bacilliform Virus by Polymerase Chain Reaction for Assessing Mild Infection of Plants and Viruliferous Vector Leafhoppers. Phytopathology 83: 655–659.

Verma, V. & I. Dasgupta. 2007. Sequence Analysis of the Complete Genomes of Two Rice Tungro Spherical Virus Isolates from India. Archives of Virology 152: 645–648.

Widiarta, I.N. & D. Kusdiaman. 2002. Identifikasi Strain Virus Tungro, p. 61–62. In H.M. Toha, Satoto, A. Setyono, Sudir, & O.S. Lesmana (eds.), Laporan Tahunan 2002. Balai Penelitian Tanaman Padi, Sukamandi.

Widiarta, I.N., Yulianto, & A. Hasanuddin. 2003. Pengendalian Terpadu Penyakit Tungro dengan Strategi Eliminasi Peranan Virus Bulat. Kebijakan Perberasan dan Inovasi Teknologi Padi. p. 513–527. In B. Suprihatno, A.K. Makarim, I.N. Widiarta, Hermanto, & A.S. Yahya. Buku 2: Kebijakan Perberasan dan Inovasi Teknologi Padi. Puslitbangtan.

Widiarta, I.N., Burhanuddin, A.A. Daradjat, & A. Hasanuddin. 2004. Status dan Program Penelitian Pengendalian Terpadu Penyakit Tungro. p. 61–89. In A. Hasanuddin, I.N. Widiarta, & Sunihardi (eds.), Strategi Pengendalian Penyakit Tungro: Status dan Program, Prosiding Seminar Nasional Status Program Penelitian Tungro Mendukung Keberlanjutan Produksi Padi Nasional. Makassar, 7–8 September 2004.

Zhang, S., G. Lee, J.W. Davies, & R. Hull. 1997. Variation in Coat Protein Genes among Five Geographically Different Isolates of Rice Tungro Spherical Virus. Archives of Virology 142: 1873–1879.



DOI: https://doi.org/10.22146/jpti.11763

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