Identifikasi dan Virulensi Fusarium oxysporum f.sp. cubense RAS 4

https://doi.org/10.22146/jpti.11748

Dwi Kiswanti(1*), Suryanti Suryanti(2), Christanti Sumardiyono(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The aim of this study was to identify and to detect the virulence of Fusarium oxysporum f.sp. cubense (Foc) race 4. The isolates consisted of BNT-2, KD-1, U-8, BK, A-2, and A-13. Monospore isolation was done to obtain stable isolates. The detection and characteristics of isolates were observed on Komada medium. The diameter of colony and color was observed from underside of culture in petridish, while the shape of colony was observed from the upper side. Virulence test was conducted on Cavendish cultivar seedlings. Banana seedlings were inoculated with Foc cultured on rice medium (20 g/kg soil). The result indicated that A-13, U-8, BNT-2, and BK, were very virulent isolates; while A-2 and KD-1 were virulent. All isolates were detected as Foc race 4, with mild yellowish color and laccinated colonies on Komada medium.


Keywords


Foc race 4; Komada medium; virulence

Full Text:

PDF


References

Djatnika, I. & W. Nuryani. 1995. Pengendalian Biologi Penyakit Layu Fusarium pada Pisang dengan Beberapa Isolat Pseudomonas fluorescens. Kongres Nasional XIII dan Seminar Ilmiah PFI, Mataram, 27–29 September 1995.

Komada, H. 1975. Development of a Selective Medium for Quantitative Isolation of Fusarium oxysporum from Natural Soil. Review of Plant Protection Research 8: 114–125.

Mak, C., A.A. Mohamed, K.W. Liew, & Y.W. Ho 2004. Early Screening Technique for Fusarium Wilt Resistance in Banana Micropropagated Plants. Banana Improvement. http://www.fao.org./docrep/ 007/ae216e00.HTM, modified 25/05/09.

Molina, A. B., E. Fabreger, V.G. Sinohin, G. Yi, & A. Viljoen. 2009. Recent Occurrence of Fusarium oxysporum f.sp. cubense Tropical Race 4 in Asia. Http://www.actahort.org, modified 25/11/09.

Nasir, N. & Jumjunidang. 2003. Karakterisasi Ras Fusarium oxysporum f.sp. cubense dengan Metode Vegetative Compatibility Group Test dan Identifikasi Kultivar Pisang yang Terserang. Jurnal Hortikultura 13: 276–284.

Pegg, K.G., R.G. Shivas, N.Y.Moore, & S. Bentley. 1995. Characterization of a Unique Population of Fusarium oxysforum f.sp. cubense Causing FusariumWilt in Cavendish Bananas at Carnarvon, Western Australia. Australian Journal of Agriculture Research 46: 167–178.

Qi, Y.X., X. Zhang, J.J. Pu., Y.X. Xie., H.Q. Zhang & S.L. Huang. 2008. Race 4 Identification of Fusarium oxysporum f.sp. cubense from Cavendish Cultivars in Hainan Province, China. Australian Plant Diseases Notes 3: 46–47.

Semangun, H. 2007. Penyakit-Penyakit Tanaman Hortikultura di Indonesia. Edisi Kedua. Gadjah Mada University Press. Yogyakarta. 850 p.

Siallagan, B. 2008. Uji Virulensi Beberapa Isolat Fusarium oxysporum f.sp. cubense. Skripsi. Fakultas Pertanian Universitas Gadjah Mada. Yogyakarta. (Unpublished).

Sun, E.J., H.J Su, & W.H. Ko. 1978. Identification of Fusarium oxysporum f.sp. cubense Race 4 from Soil or Host Tissue by Cultural Characters. Phythopathology 68: 1672–1673.

Wibowo, A., S. Subandiyah, C. Sumardiyono, L. Sulistyowati, P. Taylor, & M. Fegan. 2007. Diversitiy of Race 4 Fusarium oxysporum f. sp. cubense Strains from Indonesia. In Sumardiyono Y.B. & S. Hartono (eds.) Proceedings the Third Asian Conference on Plant Pathology. Faculty of Agriculture. Gadjah Mada University. Yogyakarta, Indonesia, August 20–24, 2007.



DOI: https://doi.org/10.22146/jpti.11748

Article Metrics

Abstract views : 6007 | views : 4440

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2010 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics