Pemurnian dan Karakterisasi Enzim Endokitinase dari Agen Pengendali Hayati Trichoderma reesei

https://doi.org/10.22146/jpti.10074

Harjono Harjono(1*), S. M. Widyastuti(2), S. Margino(3)

(1) Fakultas Kehutanan Universitas Gadjah Mada
(2) Fakultas Kehutanan Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


This experiment was aimed to purify and characterize the endochitinase of Trichoderma reesei. Extracellular endochitinase was produced by T. reesei strain T13, a fungal biocontrol agent in colloidal chitin medium as sole carbon source. The enzyme was purified by ammonium sulfate precipitation, followed by gel filtration chromatography and chromatofocusing. The results showed that T. reesei produced endochitinase with molecular weight of 32 kDa and the activity was optimum at pH of 5,5 and temperature of 30 to 35oC.


Keywords


endochitinase; Trichoderma reesei

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References

de La Cruz, J., M. Rey, J.M. Lora, A. Hidalgo-Gallego, F. Dom inguez, J.A. Pintor-Toro, A. Liobell & T. Benitez. 1993. Carbon Source Control on β-glucanase, Chitobiase and Chitinase from Trichoderma harzianum. Arch. Microbiol. 159: 316─322.

Haran, S., H. Schickler, A. Oppenheim & I. Chet. 1995. New Components of the Chitinolytic System of Trichoderma harzianum. Mycol. Res. 99: 441─446.

Haran, S., H. Schickler, A. Oppenheim & 1. Chet. 1996. Differential Expression of Trichoderma harzianuni Chitinases during Mycoparasitism. Phytopathology 86: 980─985.

Harjono & S.M. Widyastuti. 2001. Optimasi Produksi Endokitinase dan Jamur Mikoparasit Trichoderma reesei. J. Perlin. Tan. Ind. 7 (1): 55─58.

Harman, G.E., C.K. Hayes, M. Lorito, R.M. Broadway, A. Di Pietro, C. Peterbauer & A. Tronsmo. 1993. Chitinolytic Enzymes of Trichoderma harzianum: Purification of Chitobiosidase and Endochitinase. Phytopathology 83: 313─318.

Hartree, E.F. 1972. Determination of Protein: A Modification of The Lowry Method that Gives A Linier Photometric Response. Anal. Biochem. 48: 422─427.

Laemmli, U.K. 1970. Cleavage of Structural Proteins during The Assembly of The Head of Bacteriophage T4. Nature 227: 680─685.

Lorito, M., G.E. Harman, C.K. Hayes, R.M. Broadway, A. Tronsmo, S.L. Woo & A. Di Pietro. 1993a. Chitinolytic Enzymes Produced by Trichoderma harzianum: Antifungal Activity of Purified Endochitinase and Chitobiosidase. Phytopathology 83: 302─307.

Lorito, M., A. Di Pietro, C.K. Hayes, S.L. Woo & G.E. Harman. 1993b. Antifungal, Synergistic Interaction between Chitinolytic Enzymes from Trichoderma harzianum and Enterobacter cloacae. Phytopathology 83: 721─728.

Lorito, M., C.K. Hayes, A. Di Pietro, S.L. Woo & G.E. Harman. 1994. Purification, Characterization and Synergistic Activity of A Glucan 1 ,3-β-glucosidase and An N-acetyl-β-glucosamidinase from Trichoderma harzianuni. Phytopathology 84: 398─405.

Lorito, M. 1998. Chitinolytic Enzymes and their Genes, p. 73─99. In Harman, G.E. & C.P. Kubicek (Eds.), Trichoderma and Gliocladium Vol. 2. Enzymes, Biological Control and Commercial Application. Taylor and Francis, London.

Tronsmo, A. & G.E. Harman. 1993. Detection and Quantification of N-acetyl-β-D-glucosamidinase, Chitobiosidase and Endochitinase in Solution and on Gels. Anal. Biochem. 208: 74─79.

Widyastuti, S.M., Sumardi, A. Sulthoni & Harjono. 1998a. Pengendalian Hayati Penyakit Akar Merah pada Akasia dengan Trichoderma. J. Perlin. Tan. Ind. 4 (2): 65─72.

Widyastuti, S.M., Sumardi & N. Hidayati. 1 998b. Kemampuan Trichoderma spp. untuk Pengendalian Hayati Jamur Akar Putih pada Acacia mangium secara in vitro. Bul Kehut. 36: 24─38.

Widyastuti, S.M., Sumardi & Harjono. 1999. Potensi Antagonistik Tiga Trichoderma spp. terhadap Delapan Penyakit Akar Tanaman Kehutanan. Bul. Kehut. 41: 2─10.

Widyastuti, S.M., Sumardi & P. Sumantoro. 2001. Efektivitas Trichoderma spp. sebagai Pengendali Hayati terhadap Tiga Patogen Tular Tanah pada Beberapa Jenis Tanaman Kehutanan. J. Perlin. Tan. Ind. 7(2): 98─107.



DOI: https://doi.org/10.22146/jpti.10074

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