Efek asam alfa lipoat terhadap insulitis pada tikus diabetes melitus tipe 2
Ismawati Ismawati(1*), Mukhyarjon Mukhyarjon(2), Ilhami Romus(3), Sonia Dinda Paramitha(4)
(1) Kelompok Jabatan Fungsional Dosen Biokimia, Fakultas Kedokteran Universitas Riau
(2) Kelompok Jabatan Fungsional Dosen Ilmu Penyakit Dalam, Fakultas Kedokteran Universitas Riau
(3) Kelompok Jabatan Fungsional Dosen Patologi Anatomi, Fakultas Kedokteran Universitas Riau
(4) Program Studi Kedokteran, Fakultas Kedokteran Universitas Riau
(*) Corresponding Author
Abstract
Effect of alpha lipoic acid on insulitis in type 2 diabetic rat
Background: The damaging of β cell causes hyperglycemia. Β cell damaged as insulitis happens because of the increase of free radical and the decrease of endogen antioxidant that caused oxidative stress.
Objective: The goal of this research was to find out the effect of alpha lipoic acid (ALA) on pancreas Langerhans island’s histopathology in type 2 diabetic rats.
Methods: This was an experimental laboratory study with post test only design. Fifteen adult male rats of Wistar strain were segregated into three groups (n=5) labeled as control, type 2 diabetes (DM), and DM+ALA. The experiment was designed for 3 weeks. The measured parameter was insulitis level on pancreas Langerhans island of groups labeled.
Results: The statistical test result showed there was the significant difference between control and type 2 diabetes group (p=0,005), but there was no significant difference between DM and DM+ ALA group (p=0,549).
Conclusions: Although not statistically significant, giving ALA 60 mg/kg body weight for 3 weeks decreased the degree of insulitis in diabetic rats.
Background: The damaging of β cell causes hyperglycemia. Β cell damaged as insulitis happens because of the increase of free radical and the decrease of endogen antioxidant that caused oxidative stress.
Objective: The goal of this research was to find out the effect of alpha lipoic acid (ALA) on pancreas Langerhans island’s histopathology in type 2 diabetic rats.
Methods: This was an experimental laboratory study with post test only design. Fifteen adult male rats of Wistar strain were segregated into three groups (n=5) labeled as control, type 2 diabetes (DM), and DM+ALA. The experiment was designed for 3 weeks. The measured parameter was insulitis level on pancreas Langerhans island of groups labeled.
Results: The statistical test result showed there was the significant difference between control and type 2 diabetes group (p=0,005), but there was no significant difference between DM and DM+ ALA group (p=0,549).
Conclusions: Although not statistically significant, giving ALA 60 mg/kg body weight for 3 weeks decreased the degree of insulitis in diabetic rats.
Keywords
alpha lipoic acid; diabetes mellitus; insulitis
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- Setiati S, Idrus A, Suyodo AW, Simadibrata M, Setiyohadi BA, Syam AF. Ilmu penyakit dalam. Jakarta: Interna Publishing; 2014.
- Fatimah RN. Diabetes melitus tipe 2. Majority: Medical Journal of Lampung University 2015;4(5):93-101.
- Kemenkes RI. Riset kesehatan dasar. Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan Republik Indonesia; 2013.
- Szkudelski T. Streptozotocin–nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Exp Biol Med. 2012;237(5):481-90. doi: 10.1258/ebm.2012.011372
- Ghasemi A, Khalifi S, Jedi S. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes. Acta Physiol Hung. 2014;101(4):408-20. doi: 10.1556/APhysiol.101.2014.4.2
- Donath MY, Schumann DM, Faulenbach M, Ellingsgaard H, Perren A, Ehses JA. Islet inflammation in type 2 diabetes: from metabolic stress to therapy. Diabetes Care. 2008;31 Suppl 2:S161-4. doi: 10.2337/dc08-s243
- Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J. The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos Univ Med J. 2012;12(1):5-18. doi: 10.12816/0003082
- Gomes MB, Negrato CA. Alpha-lipoic acid as a pleiotropic compound with potential therapeutic use in diabetes and other chronic diseases. Diabetol Metab Syndr. 2014;6(1):80. doi: 10.1186/1758-5996-6-80
- Poh ZX, Goh KP. A current update on the use of alpha lipoic acid in the management of type 2 diabetes mellitus. Endocr Metab Immune Disord Drug Targets 2009;9(4):392-8. doi: 10.2174/187153009789839147
- Kandeil MA, Amin KA, Hassanin KA, Ali KM, Mohammed ET. Role of lipoic acid on in insulin resistance and leptin in experimentally diabetic rats. J Diabetes Complications. 2011;25(1):31-8. doi: 10.1016/j.jdiacomp.2009.09.007
- Salama RHM. Hypoglycemic effect of lipoic acid, carnitine and nigella sativa in diabetic rat model. Int J Health Sci (Qassim). 2011 Jul;5(2):126-34.
- Annadurai T, Muralidharan AR, Joseph T, Hsu MJ, Thomas PA, Geraldine P. Antihyperglycemic and antioxidant effects of a flavanone, naringenin, in streptozotocin-nicotinamide-induced experimental diabetic rats. J Physiol Biochem. 2012;68(3):307-18. doi: 10.1007/s13105-011-0142-y
- Ismawati I, Winarto W, Sari RP. Pencegahan lesi aterosklerosis oleh asam alfa lipoat pada aorta mencit jantan (Mus musculus) yang diberi diet tinggi kolesterol. JIK (Jurnal Ilmu Kedokteran). 2011;5(1):19-25.
- Gutierrez DA, Fu W, Schonefeldt S, Feyerabend TB, Ortiz-Lopez A, Lampi Y, et al. Type 1 diabetes in NOD mice unaffected by mast cell deficiency. Diabetes. 2014;63(11):3827-34. doi: 10.2337/db14-0372
- Wang H, Xue Y, Wang B, Zhao J, Yan X, Zhu MJ, et al. Maternal obesity exacerbates insulitis and type 1 diabetes in non-obese diabetic mice. Reproduction. 2014;148(1):73-9. doi: 10.1530/REP-13-0614
- Rathinavelusamy P, Mazumder PM, Sasmal D, Jayaprakash V. Evaluation of in silico, in vitro α-amylase inhibition potential and antidiabetic activity of Pterospermum acerifolium bark. Pharm Biol. 2014;52(2):199-207. doi: 10.3109/13880209.2013.823551
- Böni‐Schnetzler M, Ehses JA, Faulenbach M, Donath MY. Insulitis in type 2 diabetes. Diabetes Obes Metab. 2008;10(s4):201-4. doi: 10.1111/j.1463-1326.2008.00950.x
- Palsamy P, Subramanian S. Ameliorative potential of resveratrol on proinflammatory cytokines, hyperglycemia mediated oxidative stress, and pancreatic β‐cell dysfunction in streptozotocin‐nicotinamide‐induced diabetic rats. J Cell Physiol. 2010;224(2):423-32. doi: 10.1002/jcp.22138
- Padmalayam I, Hasham S, Saxena U, Pillarisetti S. Lipoic acid synthase (LASY): a novel role in inflammation, mitochondrial function, and insulin resistance. Diabetes. 2009 Mar;58(3):600-8. doi: 10.2337/db08-0473
- Goraca A, Huk-Kolega H, Piechota A, Kleniewska P, Ciejka E, Skibska B. Lipoic acid - biological activity and therapeutic potential. Pharmacol Rep. 2011;63(4):849-58. doi: 10.1016/s1734-1140(11)70600-4
DOI: https://doi.org/10.22146/ijcn.31701
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