Efek asam alfa lipoat terhadap insulitis pada tikus diabetes melitus tipe 2

https://doi.org/10.22146/ijcn.31701

Ismawati Ismawati(1*), Mukhyarjon Mukhyarjon(2), Ilhami Romus(3), Sonia Dinda Paramitha(4)

(1) Kelompok Jabatan Fungsional Dosen Biokimia, Fakultas Kedokteran Universitas Riau
(2) Kelompok Jabatan Fungsional Dosen Ilmu Penyakit Dalam, Fakultas Kedokteran Universitas Riau
(3) Kelompok Jabatan Fungsional Dosen Patologi Anatomi, Fakultas Kedokteran Universitas Riau
(4) Program Studi Kedokteran, Fakultas Kedokteran Universitas Riau
(*) Corresponding Author

Abstract


Effect of alpha lipoic acid on insulitis in type 2 diabetic rat

Background: The damaging of β cell causes hyperglycemia. Β cell damaged as insulitis happens because of the increase of free radical and the decrease of endogen antioxidant that caused oxidative stress.

Objective: The goal of this research was to find out the effect of alpha lipoic acid (ALA) on pancreas Langerhans island’s histopathology in type 2 diabetic rats.

Methods: This was an experimental laboratory study with post test only design. Fifteen adult male rats of  Wistar strain were segregated into three groups (n=5) labeled as control, type 2 diabetes (DM), and DM+ALA. The experiment was designed for 3 weeks. The measured parameter was insulitis level on pancreas Langerhans island of groups labeled.

Results: The statistical test result showed there was the significant difference between control and type 2 diabetes group (p=0,005), but there was no significant difference between DM and DM+ ALA group (p=0,549).

Conclusions: Although not statistically significant, giving ALA 60 mg/kg body weight for 3 weeks decreased the degree of insulitis in diabetic rats.

Keywords


alpha lipoic acid; diabetes mellitus; insulitis

Full Text:

PDF


References

  1. Setiati S, Idrus A, Suyodo AW, Simadibrata M, Setiyohadi BA, Syam AF. Ilmu penyakit dalam. Jakarta: Interna Publishing; 2014.
  2. Fatimah RN. Diabetes melitus tipe 2. Majority: Medical Journal of Lampung University 2015;4(5):93-101.
  3. Kemenkes RI. Riset kesehatan dasar. Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan Republik Indonesia; 2013.
  4. Szkudelski T. Streptozotocin–nicotinamide-induced diabetes in the rat. Characteristics of the experimental model. Exp Biol Med. 2012;237(5):481-90. doi: 10.1258/ebm.2012.011372
  5. Ghasemi A, Khalifi S, Jedi S. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes. Acta Physiol Hung. 2014;101(4):408-20. doi: 10.1556/APhysiol.101.2014.4.2
  6. Donath MY, Schumann DM, Faulenbach M, Ellingsgaard H, Perren A, Ehses JA. Islet inflammation in type 2 diabetes: from metabolic stress to therapy. Diabetes Care. 2008;31 Suppl 2:S161-4. doi: 10.2337/dc08-s243
  7. Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J. The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos Univ Med J. 2012;12(1):5-18. doi: 10.12816/0003082
  8. Gomes MB, Negrato CA. Alpha-lipoic acid as a pleiotropic compound with potential therapeutic use in diabetes and other chronic diseases. Diabetol Metab Syndr. 2014;6(1):80. doi: 10.1186/1758-5996-6-80
  9. Poh ZX, Goh KP. A current update on the use of alpha lipoic acid in the management of type 2 diabetes mellitus. Endocr Metab Immune Disord Drug Targets 2009;9(4):392-8. doi: 10.2174/187153009789839147
  10. Kandeil MA, Amin KA, Hassanin KA, Ali KM, Mohammed ET. Role of lipoic acid on in insulin resistance and leptin in experimentally diabetic rats. J Diabetes Complications. 2011;25(1):31-8. doi: 10.1016/j.jdiacomp.2009.09.007
  11. Salama RHM. Hypoglycemic effect of lipoic acid, carnitine and nigella sativa in diabetic rat model. Int J Health Sci (Qassim). 2011 Jul;5(2):126-34.
  12. Annadurai T, Muralidharan AR, Joseph T, Hsu MJ, Thomas PA, Geraldine P. Antihyperglycemic and antioxidant effects of a flavanone, naringenin, in streptozotocin-nicotinamide-induced experimental diabetic rats. J Physiol Biochem. 2012;68(3):307-18. doi: 10.1007/s13105-011-0142-y
  13. Ismawati I, Winarto W, Sari RP. Pencegahan lesi aterosklerosis oleh asam alfa lipoat pada aorta mencit jantan (Mus musculus) yang diberi diet tinggi kolesterol. JIK (Jurnal Ilmu Kedokteran). 2011;5(1):19-25.
  14. Gutierrez DA, Fu W, Schonefeldt S, Feyerabend TB, Ortiz-Lopez A, Lampi Y, et al. Type 1 diabetes in NOD mice unaffected by mast cell deficiency. Diabetes. 2014;63(11):3827-34. doi: 10.2337/db14-0372
  15. Wang H, Xue Y, Wang B, Zhao J, Yan X, Zhu MJ, et al. Maternal obesity exacerbates insulitis and type 1 diabetes in non-obese diabetic mice. Reproduction. 2014;148(1):73-9. doi: 10.1530/REP-13-0614
  16. Rathinavelusamy P, Mazumder PM, Sasmal D, Jayaprakash V. Evaluation of in silico, in vitro α-amylase inhibition potential and antidiabetic activity of Pterospermum acerifolium bark. Pharm Biol. 2014;52(2):199-207. doi: 10.3109/13880209.2013.823551
  17. Böni‐Schnetzler M, Ehses JA, Faulenbach M, Donath MY. Insulitis in type 2 diabetes. Diabetes Obes Metab. 2008;10(s4):201-4. doi: 10.1111/j.1463-1326.2008.00950.x
  18. Palsamy P, Subramanian S. Ameliorative potential of resveratrol on proinflammatory cytokines, hyperglycemia mediated oxidative stress, and pancreatic β‐cell dysfunction in streptozotocin‐nicotinamide‐induced diabetic rats. J Cell Physiol. 2010;224(2):423-32. doi: 10.1002/jcp.22138
  19. Padmalayam I, Hasham S, Saxena U, Pillarisetti S. Lipoic acid synthase (LASY): a novel role in inflammation, mitochondrial function, and insulin resistance. Diabetes. 2009 Mar;58(3):600-8. doi: 10.2337/db08-0473
  20. Goraca A, Huk-Kolega H, Piechota A, Kleniewska P, Ciejka E, Skibska B. Lipoic acid - biological activity and therapeutic potential. Pharmacol Rep. 2011;63(4):849-58. doi: 10.1016/s1734-1140(11)70600-4



DOI: https://doi.org/10.22146/ijcn.31701

Article Metrics

Abstract views : 3039 | views : 3390

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2019 Jurnal Gizi Klinik Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Gizi Klinik Indonesia (JGKI) Indexed by:
 
  

  free web stats View My Stats