ANALISA JUMLAH KROMOSOM IKAN MAS KOKI (Carrasius auratus) TETRAPLOID YANG DIHASILKAN DENGAN METODE KEJUTAN PANAS

https://doi.org/10.22146/jfs.9037

Rustidja Rustidja(1*)

(1) Fakultas Perikanan Universitas Brawijaya
(*) Corresponding Author

Abstract


The objectives of research was to analyses the chromosome numbers of tetraploid goldfish (Carassius auratus) produced by heat shocking method. The tetraploidy was carried out 29 minutes after fertilization of eggs by heat shocking for 1.5 minutes at 400C.The chromosome number was analyzed with the modus of chromosomes.

The results indicated that 1)  the diploid control of goldfish has 100.28% + 0.0982 chromosom  with modus numbers of  100, and the tetraploid goldfish has 199.94 + 0.982 chromosomes with number of modus at 200. 2)  The effectiveness of the heat shocking method in goldfish for tetraploidy was 46.60%.

The hatching rate of diploid control (65.068% + 0.52%) was significantly diferent with that of  tetraploid fish (24.53% + 1.649%). In addition, the survival rate of diploid controls (86.875% + 6.287). was significantly different with that of tetraploid goldfish (34.274% + 19.289%) and the specific growth rates of diploid controls (5,104 + 0,074) was not significantly diferent that of with tetraploid fish (3.982 + 0.011). 


Keywords


Carrasius auratus, chromosoms, heat shocking method, tetraploid

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References

Al-Sabti. 1985. Chromosomal studies by blood leucocyte culture tech-nique on three salmonids from Yugoslavian waters. Fish Biol, 26 : 5 – 12

Carman O., Takashi and T. Fumio, 1991. Estimation of effective management condition for induction of triploidy in goldfish, Carasius auratus. J. of the Tokyo Univ. of Fisheries, 78 (2) : 127-135.

Carman O. 1992. Variation in the maximum number of nucleoli Departement Aquatic Bio-Sciences. Tokyo Univ. of Fisheries. Tokyo.

Chingjiang, W., Y. Yuzhen and C. Rongde. 1986. Genom mani-pulation in carp. (Cyprinus carpio L.) Aquaculture. 54 : 57 – 61.

Denton, T.E., and W.M.Howell, 1965. A technique for obtain chromo-some from the scale epithelium of fucos fishes. Dept. of Biology Stanford Univ. of Alabama. 392 – 393.

De Robertis, E.D. Pand and E.M.F. De Robertis, 1981. Essential of cell and mollecular biology. Saun-ders Collage Publ. Holt-Saunders Tokyo. 395 p.

Effendi, H. 1990. Memelihara mas koki di akuarium. Kanisius. Yogyakarta. 151 hal.

Gervai J.S., A. Nagy, S. Peter, L. Hoorvath and V. Cassani. 1980. Induced triploidy in carp. J. Fish Biol. 17 : 667 – 671.

Gunarso, W. 1986. Penuntun praktikum sitogenetika. P.A.U. IPB Bogor. 14-16 p.

Najmushabah., F. 1993. Pengaruh kejut-an panas terhadap keberhasilan triploidy ikan lele (Clarias batrachus L). Fakultas Perikanan IPB.

Rustidja. 1991. Aplikasi manipulasi kromosom pada program pem-benihan ikan. Makalah pada kongres ilmu pengetahuan nasional V di Jakarta, September 1991. 23 p.

Suryo. 1990. Sitogenetika. Gajah Mada Univ. Press. Yogyakarta. 442 p.

Susanto, H. 1987. Budidaya ikan di pekarangan. PT. Penebar Swadaya. Jakarta. 112 p.



DOI: https://doi.org/10.22146/jfs.9037

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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