Interleukin-8 expression differences in chronic and active chronic inflammation gastric mucosa biopsy with helicobacter pylori infection
Muhammad Arif(1*), Siti Nurdjanah(2), Neneng Ratnasari(3)
(1) 
(2) 
(3) 
(*) Corresponding Author
Abstract
Background: Infection with Helicobacter pylori (HP) estimated to occur in 50% of the population in the world. Helicobacter pylori infection causes inflammation of the gastric mucosa and gastric epithelial release of interleukin-1β, interleukin-6, interleukin-8 and tumor necrosis factor α. Interleukin-8 plays a role in the degree of chronic inflammation of the gastric mucosa and gastric cancer risk. There has been no research on differences in the expression of interleukin-8 is based on chronic and chronic active inflammation on biopsy of the gastric mucosa with Helicobacter pylori infection in Hospital Dr. Sardjito.
Objective: To prove the differences in the expression of interleukin-8 of chronic inflammation and gastric mucosal biopsy active chronic infection with Helicobacter pylori.
Methods: This study used a cross-sectional design. Examined the expression of interleukin-8 on the rest of the biopsy sample HP (+) in August 2009 to March 2014. Classification of chronic inflammatory and chronic active obtained through histopathology report. Categorical numerical data from the two groups, unpaired, the distribution is not normal: Mann-Whitney test. Differences were considered significant if it was obtained p <0.05 with a confidence interval of 95%.
Results: There were 41 samples of gastric mucosa biopsy preparations, comprising 9 samples of chronic inflammation and 32 samples of chronic inflammatory active of the gastric mucosa. The median value of the expression of IL-8 eptitel surface on chronic inflammation by 83 (40-94)% and the active chronic inflammation by 82 (19-94). Comparison test with Mann Whitney U test. From the statistical calculations p value = 0.887 with p> 0.05
Conclusion: There is no difference in the expression of interleukin-8 in inflammatory chronic active and chronic inflammation of the gastric mucosa with Helicobacter pylori infection.
Keywords: Helicobacter pylori, interleukin-8, chronic inflammation and chronic inflammatory active.
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Arrosy, Ratnasari, N., Indrarti, F., 2012, Prevalence Of Helicobacter pylori Infection Based On Histopathology Of Gaster And Duodenum In Dr. Sardjito Hospital, Yogyakarta September 2009-September 2010.
Versalovic J., 2003, Helicobacter pylori Pathology and Diagnostic Strategies. Am J Clin Pathol, 119:403-12.
Kozlowski W., Jochymski C. & Markiewicz T., 2006. Chronic Gastritis: Gastritis and Gastric Cancer – New Insights in Gastroprotection, Diagnosis and Treatments, 5:75-92.
Wroblewski, L., Peek, R., Wilson, K.T., 2010, Helicobacter Pylori And Gastric Cancer: Factors That Modulate Disease Risk, Clinical Microbiology Reviews, 23 (4) 713–739.
Gionchetti, P., Vaira, D., Campieri, M., Holton, J.,1994, Enhanced Mucosal Interleukin-6 And -8 In Helicobacter Pylori Positive Dyspeptic Patients., Am J Gastroenterol, 89(6):883-887.
Suerbaum, S., Michetti, P., 2002, Helicobacter Pylori Infection, N Engl J Med, 347 (15) : 1175-1186
Hannelien, V., Karel , G., Sofie, S., 2012, The Role Of Cxc Chemokines In The Transition Of Chronic Inflammation To Esophageal And Gastric Cancer, Biochimica Et Biophysica Acta 1825, 117–129
Yamaoka, Y., Kodama, T., Kita, M., Imanishi, J., Kashima,K., 1999, Relation Between Clinical Presentation, Helicobacter Pylori Density, Interleukin 1β And 8 Production, And CagA Status, Gut; 45 : 804–811
Bakir, W.A., Hussain, A., Faisal, M., 2008, Gastric Mucosal Interleukine-8 (IL-8) And Interleukine-1beta (IL-1ß) Levels In Atrophic Gastritis And Gastric Carcinoma Patients., J Fac Med Baghdad. 50 (4)
Oh, Y.S., Hong, S.J., Kim, J.O., Cho, J.Y., Lee, J.S., 2002, Effect of Helicobacter pylori Infection on Mucosal Interleukin-8 Expression in Gastric Metaplasia, Korean J Gastroenterol, 39(3):161-166.
Mariadi, I.K., Kurniari, P.K.,Wibawa,I.D.N., Purwadi, 2011, Interleukin 8 Berhubungan dengan Derajat Gastritis padaPasienTerinfeksi Helicobacter Pylori, J PenyDalam, Volume 12 Nomor 2, 98-102
Taguchi, A., Ohmiya, N., Shirai, K., Mabuchi, N., Itoh, A., Hirooka, Y., 2005, Interleukin-8 Promoter Polymorphism Increases the Risk of Atrophic Gastritis and Gastric Cancer in Japan, Cancer Epidemiol Biomarkers Prev; 14(11) : 2487-93.
Patel, S.R., 2011, Determinants Of Helico Bader Pylori Density In The Human Stomach And Implications In Diseas, University Of Nottingham
Shimoyama, T., Everett, S.M., Fukuda, S., Dixon, M.F., Crabtree. J.E., 2001, Influence Of Smoking And Alcohol On Gastric Chemokine Mrna Expression In Patients With Helicobacter Pylori Infection, J Clin Pathol ; 54:p332–334
Graham, Opekun, A.R., Yamaoka, Y., Osato, M.S., El-Zimaity, H.M. 2003, Early Events in Proton Pump Inhibitor-Associated Exacerbation of Corpus Gastritis, Aliment Pharmacol Ther, 17: 193–200
Moshkowitz,M., Horowitz,N., Beit-Or,A., Halpern,Z, Santo,E 2012. Gender Associated differences in urea breath test for Helicobacter Pylori Infection referrals and results among dyspeptic patients, World J Gastrointestinal Pathophysiology, vol3, p80-4
Kato S, et all. 2004. Sex differences in mucosal response to Helicobacter pylori infection in the stomach and variations in interleukin-8, COX-2 and trefoil factor family 1 gene expression. Aliment Pharmacol Ther 2004; 20 (Suppl. 1): 17–24.
DOI: https://doi.org/10.22146/actainterna.48161
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