Cytotoxic Dammarane-Type Triterpenoids from  Aglaia cucullata  Peel Fruit

Intan Hawina Anjari; Desi Harneti; Kindi Farabi; Al Arofatus Naini; Ace Tatang Hidayat; Risyandi Anwar; Hadi Kuncoro; Mohamad Nurul Azmi; Unang Supratman

doi:10.22146/ijc.83694

Cytotoxic Dammarane-Type Triterpenoids from Aglaia cucullata Peel Fruit

https://doi.org/10.22146/ijc.83694

Intan Hawina Anjari⁽¹⁾, Desi Harneti⁽²⁾, Kindi Farabi⁽³⁾, Al Arofatus Naini⁽⁴⁾, Ace Tatang Hidayat⁽⁵⁾, Risyandi Anwar⁽⁶⁾, Hadi Kuncoro⁽⁷⁾, Mohamad Nurul Azmi⁽⁸⁾, Unang Supratman^(9*)

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia; Central Laboratory of Universitas Padjadjaran, Jl. Raya Bandung-Sumedang, Km. 21, Jatinangor, Sumedang 45363, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(5) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia; Central Laboratory of Universitas Padjadjaran, Jl. Raya Bandung-Sumedang, Km. 21, Jatinangor, Sumedang 45363, Indonesia
(6) Herbal Medicine Research, Department of Paediatric Dentistry, Faculty of Dental Medicine, University of Muhammadiyah Semarang, Semarang 50272, Indonesia
(7) Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75123, Indonesia
(8) School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
(9) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia; Central Laboratory of Universitas Padjadjaran, Jl. Raya Bandung-Sumedang, Km. 21, Jatinangor, Sumedang 45363, Indonesia
(*) Corresponding Author

Abstract

Four triterpenoids, known as dammarane-type, dammaradienone (1), 20(S),25-epoxy-5α-dammar-20-en-3-one (2), 20(S)-5α-dammar-24-en-3α,20-diol-3-acetate (3) and 3α-acetyl-20S,24S-epoxy-25-hydroxydammarane (4), were isolated from Aglaia cucullata peel fruit. The structures of isolated compounds were identified based on their HR-TOFMS data and extensive NMR spectroscopic analysis, as well as compared with literature data. Compounds 1-4 were assessed for cytotoxic effects against HeLa cervical and B16-F10 melanoma skin cancer cells. All compounds showed moderate to weak activity against B16-F10 cancer cells, while compound 2 exhibited the strongest activity against HeLa cancer cells with IC₅₀ of 7.10 µg/mL indicating that the existence of an epoxy moiety at the side chain increases the cytotoxicity to HeLa cells.

Keywords

Aglaia cucullate; B16-F10; dammarane-type; cytotoxic activity; HeLa

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DOI: https://doi.org/10.22146/ijc.83694