Carbon Paste Electrode Modified by Dibenzo-18-crown-6 for the Determination of Paracetamol Using Differential Pulse Voltammetry Technique

Irdhawati Irdhawati; Ni Ketut Shinta Mas Methaninditya; Anak Agung Bawa Putra

doi:10.22146/ijc.74393

Carbon Paste Electrode Modified by Dibenzo-18-crown-6 for the Determination of Paracetamol Using Differential Pulse Voltammetry Technique

https://doi.org/10.22146/ijc.74393

Irdhawati Irdhawati^(1*), Ni Ketut Shinta Mas Methaninditya⁽²⁾, Anak Agung Bawa Putra⁽³⁾

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, Kampus Bukit Jimbaran, Kuta Selatan, Badung 80361, Bali, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, Kampus Bukit Jimbaran, Kuta Selatan, Badung 80361, Bali, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, Kampus Bukit Jimbaran, Kuta Selatan, Badung 80361, Bali, Indonesia
(*) Corresponding Author

Abstract

The fabrication, optimization and validation of measurement using a modified carbon paste electrode (CPE) with dibenzo-18-crown-6 were carried out for the determination of paracetamol in commercial products. The pH, dibenzo-18-crown-6 concentration in carbon paste, and scan rate parameters were optimized. Validation of the measurement was observed from linear concentration range, LoD, LoQ, precision, and percentage of the recovery. The result showed that the optimum pH was at 2, the optimum concentration of dibenzo-18-crown-6 in carbon paste was 0.6%, optimum scan rates were 35 mV/s for CPE, and increased to 50 mV/s using modified CPE. The linear concentration range for CPE was obtained at 10-300 µM with LoD and LoQ of 28.88 and 96.28 µM, respectively. Meanwhile, CPE modified with dibenzo-18-crown-6 gave wider linear concentration range at 1–700 µM with LoD and LoQ of 52.36 and 174.53 µM, respectively. The CPE and modified CPE had good precision, with Horwitz ratio values of less than two. The percentage of recovery for two samples with three replicates measurements was obtained (89.81 ± 1.38)% and (108.02 ± 0.42)% for samples A and B, respectively. Dibenzo-18-crown-6 modified CPE was used for the determination of paracetamol in both samples yielding 97–98% compared with the paracetamol composition on its labels.

Keywords

carbon paste electrode; dibenzo-18-crown-6; differential pulse voltammetry; paracetamol

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References

[1] Tulandi, G.P., Sri, S., and Widya, A.L., 2015, Validation of analytical methods for determination of paracetamol levels in tablets by ultraviolet spectrophotometry (Indonesian), Pharmacon, 4 (4), 168–178.

[2] Susilawan, I.P.N.A., Siaka, I.M., and Parwata, I.M.O.A., 2018, Validation of chemical analysis methods of paracetamol and phenylbutason in traditional medicinal products with HPTLC-spectrophotodensitometry (Indonesian), Cakra Kimia, 7 (1), 1–11.

[3] Sarmento, Z.L.C., Rangdi, O.S.G., Olivia, S.G.R., Sena, B.M.C.D., and Dewi, K.N.M., 2020, Determination of paracetamol and caffeine levels using high performance liquid chromatography (HPLC) method (Indonesian), Chakra Kimia, 8 (2), 99–104.

[4] Tanuja, S.B., Kumara Swamy, B.E., and Pai, K.V., 2017, Electrochemical determination of paracetamol in presence of folic acid at nevirapine modified carbon paste electrode: A cyclic voltammetric study, J. Electroanal. Chem., 798, 17–23.

[5] Damanik, A.N.T., 2019, Determination of paracetamol levels in tablets with trade names and generics by Fourier transform infra red (FTIR) spectrophotometry (Indonesian), Thesis, Pharmacy Study Program, University of North Sumatra, Medan.

[6] Ayalew, M.E., and Ayitegeb, D.Y., 2021, Differential pulse voltammetric determination of paracetamol using activated glassy carbon electrode, Am. J. Phys. Chem., 10 (2), 16–24.

[7] Wang, J., 2001, Analytical Electrochemistry, 2^nd Ed., Wiley-VCH, New York.

[8] Wachid, M.R., and Setiarso, P., 2014, Preparation of bentonite modified carbon paste electrodes for the analysis of copper (II) metal with lead (II) and mercury (II) interfering ions by cyclic voltammetry stripping (Indonesian), UJChem, 3 (3), 93–103.

[9] Amare, M., and Teklay, W., 2019, Voltammetric determination of paracetamol in pharmaceutical tablet samples using anthraquinone modified carbon paste electrode, Cogent Chem., 5 (1), 1576349.

[10] Salem, W.M., 2019, Sensitive determination of paracetamol using ferrocene nanoparticles by chitosan-functionalized-modified carbon paste electrode, Egypt. J. Chem., 62 (4), 1079–1090.

[11] Naik, T.S.S.K., Swamy, B.E.K., Ramamurthy, P.C., and Chetankumar, K., 2020, Poly (L-leucine) modified carbon paste electrode as an electrochemical sensor for the detection of paracetamol in presence of folic acid, Mater. Sci. Energy Technol., 3 (1), 626–632.

[12] Matt, S.B., Raghavendra, S., Shivanna, M., Sidlinganahalli, M., and Siddalingappa, D.M., 2021, Electrochemical detection of paracetamol by voltammetry techniques using pure zirconium oxide nanoparticle based modified carbon paste electrode, J. Inorg. Organomet. Polym. Mater., 31 (2), 511–519.

[13] Irdhawati, I., Manurung, M., and Septiawan, K.A., 2015, Voltammetric dopamine detection using crown ether modified carbon paste electrodes (dibenzo-18-crown-6) (Indonesian), J. Kim. Mulawarman, 12 (2), 68–74.

[14] Melson, G.A., 1979, Coordination Chemistry of Macrocyclic Compounds, Plenum Press, New York.

[15] Sadok, I., and Tyszczuk-Rotko, K., 2015, New, simple and sensitive voltammetric procedure for determination of paracetamol in pharmaceutical formulations, Insights Anal. Electrochem., 1, 1.

[16] Miller, J.N., and Miller, J.C., 2010, Statistics and Chemometrics for Analytical Chemistry, 6^th Ed., Prentice Hall, Essex, England.

[17] Horwitz, W., and Albert, R.J., 2006, The Horwitz ratio (HorRat): A useful index of method performance with respect to precision, J. AOAC Int., 89 (4), 1095–1109.

[18] AOAC International, 1998, Peer-Verified Methods Program Manual Policies and Procedures, Association of Official Analytical Chemists, Gaithersburg, MD, USA.

[19] Mulyasuryani, A., Tjahjanto, R.T., and Andawiyah, R., 2019, Simultaneous voltammetric detection of acetaminophen and caffein base on cassava starch-Fe₃O₄ nanoparticles modified glassy carbon electrode, Chemosensors, 7 (4), 49.

[20] Irdhawati, I., Manurung, M., and Maulinasari, A., 2015, Modification of carbon paste electrode with crown ether (dibenzo-18-crown-6) for ascorbic acid analysis by differential pulse voltammetry, ALCHEMY Jurnal Penelitian Kimia, 11 (2), 175–191.

DOI: https://doi.org/10.22146/ijc.74393