SIMULTANEOUS DETERMINATION OF PARACETAMOL AND IBUPROFENE MIXTURES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

https://doi.org/10.22146/ijc.21899

Sophi Damayanti(1*), Slamet Ibrahim(2), Kurnia Firman(3), Daryono H Tjahjono(4)

(1) Unit Bidang Ilmu Farmasi Analisis, Departemen Farmasi, Institut Teknologi Bandung, Bandung
(2) Unit Bidang Ilmu Farmasi Analisis, Departemen Farmasi, Institut Teknologi Bandung, Bandung
(3) Unit Bidang Ilmu Farmasi Analisis, Departemen Farmasi, Institut Teknologi Bandung, Bandung
(4) Unit Bidang Ilmu Farmasi Analisis, Departemen Farmasi, Institut Teknologi Bandung, Bandung
(*) Corresponding Author

Abstract


Analytical method for the determination of paracetamol and ibuprofene mixtures has been developed by High Performance Liquid Chromatography using C-18 column and acetinitrile - phosphate buffer pH = 4.5 (75:25) containing 0.075% sodium hexanesulfunate as a mobile phase. The detector was set at 215 nm. Using such conditions, retention time for paracetamol and ibuprofen was 4.89 and 7.11 min, respectively. The recovery for paracetamol and ibuprofen was found to be 101.07± 0.73% and 102.02 ± 1.58%, respectively. The detector limits of the method was 1.30 and 1.60 μg/mL with the relative standard deviation (RSD) 0.74 and 1.52% for paracetamol and ibuprofen, respectively.


Keywords


paracetamol; ibuprofen; multi-component; validation; HPLC

Full Text:

Full Text Pdf


References

[1] Elsayaed, M. A. H., 1976, J. Pharm. Sci., 68, 739.

[2] Morelli, B., 1988, J. Pharm. Sci., 77, 1042.

[3] Ditjen POM, DepKes RI, 1994, Farmakope Indonesia, ed. 4, DepKes RI, Jakarta, 449-450, 649-650.

[4] Ibrahim, S., 1998, Pengembangan Metode Analisis Menggunakan Kromatografi Cair Kinerja Tinggi, Seminar HPLC Application for Analysis of Drugs, Food and Environment, Bandung, 13 November 1998, 1-23.

[5] Skoog, D.A., Holler, F.J., and Nieman, T.A., 1992, Principles of Instrumental Analysis, 5th ed., Saunders College Publ., New York, 1-7, 140-167.

[6] Willard, H.H., Merrit Jr., L.L., Dean, J.A. and Settle Jr., F.A., 1988, Instrumental Method of Analysis, 7th ed., Wadsworth Publ. Co., Belmont, 159-186.



DOI: https://doi.org/10.22146/ijc.21899

Article Metrics

Abstract views : 4316 | views : 30869


Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 


Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web
Analytics View The Statistics of Indones. J. Chem.