Effect of fluidised bed drying on ginsenoside content in hairy root cultures of Panax ginseng C.A. Meyer

James Setiabudi; Komang Mega Oka Sri Bintang; Stella Stacia Gani; Pissa Christanti; Evanie Noer Putri; Se Chan Kang; Johan Sukweenadhi

doi:10.22146/ijbiotech.79677

Effect of fluidised bed drying on ginsenoside content in hairy root cultures of Panax ginseng C.A. Meyer

https://doi.org/10.22146/ijbiotech.79677

James Setiabudi⁽¹⁾, Komang Mega Oka Sri Bintang⁽²⁾, Stella Stacia Gani⁽³⁾, Pissa Christanti⁽⁴⁾, Evanie Noer Putri⁽⁵⁾, Se Chan Kang⁽⁶⁾, Johan Sukweenadhi^(7*)

(1) Faculty of Biotechnology, University of Surabaya, Raya Kalirungkut, Kalirungkut, Surabaya 60293, Jawa Timur, Indonesia
(2) Faculty of Biotechnology, University of Surabaya, Raya Kalirungkut, Kalirungkut, Surabaya 60293, Jawa Timur, Indonesia
(3) Faculty of Biotechnology, University of Surabaya, Raya Kalirungkut, Kalirungkut, Surabaya 60293, Jawa Timur, Indonesia
(4) PT. Bintang Toedjoe, Pulomas, Jakarta 13210, Indonesia
(5) PT. Bintang Toedjoe, Pulomas, Jakarta 13210, Indonesia
(6) Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea
(7) Faculty of Biotechnology, University of Surabaya, Raya Kalirungkut, Kalirungkut, Surabaya 60293, Jawa Timur, Indonesia
(*) Corresponding Author

Abstract

Korean Ginseng (Panax ginseng C.A. Meyer) is a high‐value herb with many pharmacological benefits due to its primary active compound, ginsenosides. The most ginsenosides are known to be thermolabile and susceptible to degradation at high‐temperature processing. Our previous studies revealed that the optimum parameters related to the P. ginseng tissue culture protocol, particularly for hairy root propagation of Cultured Roots of Mountain Ginseng (CRMG)‐88, was using a lab‐scale bioreactor. The next stage involves screening for a suitable post‐harvest treatment, i.e., drying, will be production of the best quality ginsenoside content. This study therefore aimed to examine the ginsenoside content by using a fluidised bed dryer (FBD) on the ginseng roots. Our results showed that FBD produced a significantly higher of total ginsenoside content (5.386 ± 1.167%), compared to control (3.750 ± 0.641%). FBD‐dried CRMG‐88 also appeared lighter in colour and more voluminous with a Loss on Drying (LOD) of 6.448 ± 1.900%. This study concluded that fluidised bed drying is superior in retaining ginsenoside content and has the potential for large‐scale application.

Keywords

Ginseng; Ginsenosides; Thermal decomposition; Tissue culture.

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DOI: https://doi.org/10.22146/ijbiotech.79677