Anti-metastatic effect of curcumin analog pentagamaboronon-0-fructose (PGB-0-F) against 4T1 breast cancer cells

Yogi Ertanto; Rohmad Yudi Utomo; Riris Istighfari Jenie; Ratna Asmah Susidarti; Edy Meiyanto

doi:10.22146/ijbiotech.36431

Anti-metastatic effect of curcumin analog pentagamaboronon-0-fructose (PGB-0-F) against 4T1 breast cancer cells

https://doi.org/10.22146/ijbiotech.36431

Yogi Ertanto⁽¹⁾, Rohmad Yudi Utomo⁽²⁾, Riris Istighfari Jenie⁽³⁾, Ratna Asmah Susidarti⁽⁴⁾, Edy Meiyanto^(5*)

(1) Biotechnology Program, Graduate School of Universitas Gadjah Mada, Jalan Teknika Utara, Sleman, Yogyakarta 55281, Indonesia; Army Medical Center, Jalan Mayjen Soetoyo, Cililitan, Jakarta 13640, Indonesia
(2) Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakarta 55281, Indonesia
(3) Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakarta 55281, Indonesia; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakarta 55281, Indonesia
(4) Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakarta 55281, Indonesia; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakarta 55281, Indonesia
(5) Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakarta 55281, Indonesia; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract

Development of a chemotherapeutic agent and boron carrying pharmaceutical based on triple-negative breast cancer is important due to its metastatic progression. Metastases are often more dangerous than the primary tumor and they are responsible for 90% of all cancer deaths. The purpose of this study was to explore the anti-metastatic activities of the PGB-0 complex with fructose (PGB-0-F) against 4T1 breast cancer cells. A scratch wound healing assay was carried out to determine the migration inhibition ability of PGB-0-F, while MMP-9 expression was analysed using gelatin zymography. The testing of anti-migration activity showed that PGB-0-F inhibited in 4T1 cells, whereas the gelatin zymography assay revealed a suppression of MMP-9 expression. PGB-0-F inhibited closure on 4T1 metastatic breast cancer cells line compared with the control. PGB-0-F decreased the MMP-9 expression level compared with the control. Based on these results, PGB-0-F has the potential to be developed as a chemotherapeutic agent, and especially as an anti-metastatic agent.

Keywords

curcumin analogue, PGB-0-F, 4T1 Cells, migration, MMP-9

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DOI: https://doi.org/10.22146/ijbiotech.36431