Molecular Identifcation of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product
https://doi.org/10.22146/ijbiotech.16368 Helen Joan Lawalata(1*), Langkah Sembiring(2), Endang Sutriswati Rahayu(3)
(1) Departement of Biology, Faculty of Mathematics and Natural Science, Manado State University, Manado, Indonesia
(2) Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Twenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditional
fermented fsh product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilage
bacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. One
isolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilage
bacteria. This strain was further identifed by 16S rRNA gen sequence comparison. Isolates LAB producing
antimicrobial agents from bakasang were identifed as Pediococcus acidilactici.
fermented fsh product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilage
bacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. One
isolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilage
bacteria. This strain was further identifed by 16S rRNA gen sequence comparison. Isolates LAB producing
antimicrobial agents from bakasang were identifed as Pediococcus acidilactici.
Keywords
Bakasang; LAB; antimicrobial; phenotypic characteristics; 16S rRNA gene
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PDF DOI: https://doi.org/10.22146/ijbiotech.16368
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