Angiotensin I-converting Enzyme (ACE) Inhibitory Activity of ACE Inhibitory Peptides Produced during the Fermentation of Pigeon Pea (Cajanus cajan) Tempe

https://doi.org/10.22146/ifnp.46921

Suci Apsari Pebrianti(1*), Muhammad Nur Cahyanto(2), Retno Indrati(3)

(1) Gadjah Mada University
(2) Faculty of Agricultural Technology, Universitas Gadjah Mada
(3) Faculty of Agricultural Technology, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Fermentation products are common sources of angiotensin I-converting enzyme (ACE) inhibitory peptides used for hypertension treatment. This research investigated the effect of fermentation time on the ACE inhibitory activity produced during the fermentation of pigeon pea tempe and aimed to determine the optimal fermentation time to obtain pigeon pea tempe with the highest ACE inhibitory activity. Seeds were inoculated with Raprima® (0.02% w/w) containing Rhizopus oligosporus spores and fermented for 0-96 h. Protein pattern, degree of hydrolysis (DH), soluble protein content and ACE inhibitory activity were observed during fermentation. The result from SDS-PAGE shows that protein hydrolysis occurred after 12 h fermentation, marked by the appearance and greater intensity of protein bands with low-molecular-weight (60 kDa). An increase in DH and soluble protein content were detected during the fermentation and reached a maximum of 23.99% and 3.15 mg mL -1 at 96 h fermentation, respectively. The ACE inhibitory activity increased with fermentation time and pigeon pea tempe fermented for 48 h (76.14%) has the highest ACE inhibitory activity with IC 50 values of 0.65 mg mL -1 . It could be concluded that the optimal fermentation time to obtained pigeon pea tempe with the highest ACE inhibitory activity is for 48 h of fermentation.


Keywords


ACE inhibitor; tempe; fermentation; pigeon pea

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References

Akubor, P.I. 2017. Effect of processing methods on the chemical composition and functional properties of pigeon pea seed. Asian Journal of Advances in Agricultural Research. 2(2):1-8.

Church, F. C., Porter, D. H., Catignani, G. L., and Swaisgood, H.E. 1985. An o- Phthalaldehyde Spectrophotometric Assay for proteinases. Analytical Biochemistry. 146:343-348.

Chusman, D. W., and Cheung, H. S. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochemical Pharmacology. 20:1637-1648.

Gibbs, B. F., Zougman, A., Masse, R., & Mulligan, C. 2004. Production and characterization of bioactive peptides from soy hydrolysate and soy fermented food. Journal of Food Research International. 37:123–131.

Hang, M dan Zhao, X.H. 2012. Fermentation time and ethanol/water-based solvent system impacted in vitro ACE-inhibitory activity of the extract of Mao-tofu fermented by Mucor spp., CyTA - Journal of Food.10(2):137-143.

Laemmli U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Journal of Nature. 227(5259): 680-685.

Lee, B. H., Yi-Syuan, L., and She-Ching, W. 2015. Antioxidation, angiotensin converting enzyme inhibition activity, nattokinase, and antihypertension of Bacillus subtilis (natto)-fermented pigeon pea. Journal of Food and Drug Analysis. 23:750-757.

Ma, Y., Cheng Y., Yin, L., Wang, J., dan Li, L. 2013. Effects of processing and NaCl on angiotensin I-converting enzyme inhibitory activity and γ- aminobutyric acid content during sulfur manufacturing. Journal of Food and Bioprocess Technology. 6:1782-1789.

Nawaz, K. A. A., David, S. M., Murugesh, E., Thandeeswaran, M., Kiran, K.G., Mahendram, R., Palaniswamy, M., and Angayarkanni, J. 2017. Identification and in silico characterization of a novel peptide inhibitor of angiotensin converting enzyme from pigeon pea (Cajanus cajan). Journal of Phytomedicin. 36:1-7.

Nogata, Y., Nagamine, T., Yanaka, M., dan Ohta, H. 2009. Angiotensin I converting enzyme inhibitory peptides produces by autolysis reactions from wheat bran. Journal of Agricultural and Food Chemistry 57:6618-6622.

Okamoto, A., Hanagata, H., Matsumoto, E., Kawamura, Y., Koizumi, Y., and Yanagida, F. 1995. Angiotensin I converting enzyme inhibitory activities of various fermented foods. Journal of Bioscience, Biotechnology, and Biochemistry. 59(6):1147-1149.

Oshodi, A.A., Olaofe, O, dan Hall, G.M. 1993. Amino acid, fatty acid and mineral composition of pigeon pea (Cajanus cajan). International Journal of Food Sciences and Nutrition. 43:187-191.

Roth, G.A., Johnson, C., Abajobir, A., Abd-Allah, F., Abera, S.F. Abyu, G., Ahmed, M., Aksut, B., Alam, T., Alam, K., Alla, F., Alvis-Guzman, N., Amrock, S., Ansari, H., Arnlov, J., Asayesh, H., Atey, T.M., Avila-Burgos, L., Awasthi, A., Banerjee, A., Barac, A., Barnighausen, T., Barregard, L., Bedi, N., Ketema, E.B., Bennet, D., Berhe, G., Bhutta, Z., Bitew, S., Carapetis, J., Carrero, J.S., Malta, D.C., Castandea-Orjuela, C.A., Castillo_Rivas, J., Catala-Lopez, F., Choi, Y.J., Christensen, H., Cirillo, M., Cooper, L., Criqui, M., Cundiff, D., Damasceno, A., Dandona, L., Davletov, K., Dharmaratne, S., Dorairaj, P., Dubey, M., Ehrenkranz, R., Esteghamati, A., Farid, T., Zaki, M.E.S., Faraon, E.J.A., Farvid, M., Feigin, V., Ding, V.L., Fowkes, G., Gebrehiwot, T., Gillum, R., Gold, A., Gona, P., Gupta, R., Habtewold, T.D., Hafezi-Nejad, N., Hailu, T., Hailu, G.B., Hankey, G., Hassen, H.Y., Abate, K.H., Havmoeller, R., Hay, S.I., Horino, M., Hotez, P.J., Jacobsen, K., James, S., Javanbakht, M., Jeemon, P., John, D., Jonas, J., Kalkonde, Y., Karimkhani, C., Kaseain, A., Khader, Y., Khan, A., Khang, Y.H., Khera, S., Khoja, A.T., Khubchandani, J., Kim, D., Kolte, D., Kosen, S., Krhon, K.J., Kumar, A., Kwan, G.F., Lal, D.K., Larsson, A., Linn, S., Lopez, A., Lotufo, P.A., Razek, H.M.A.E., Malekzadeh, R., Mazidi, R., Meier, T., Meles, K.G., Mensah, G., Meteroja, A., Mezgebe, H., Miller, T., Mirtakhimov, E., Mohammed, S., Moran, A.E., Musa, K.I., Narula, J., Neal, B., Ngalesoni, F., Nguyen, G., Obermeyer, C.M., Owolabi, M., Patton, G., Pedro, J., Qato, D., Qorbani, M., Rahimi, K., Rai, R.K., Rawaf, S., Ribiero, A., Safiri, S., Salomon, J.A., Santos, I., Santric, M., Sartorius, B., Schutte, A., Sepanlou, S., Shaikh, M.A., Shin, M.J., Shisehbor, M., Shore, H., Silva, D.A.S., Sobngwi, E., Strangers, S., Swaminathan, S., Seisdedos, R.T., Tadele, N.A., Tesfay, T., Thakur, J.S., Thrift, A., Topor-Madry, R., Truelsen, T., Tyrivolas, S., Ukwaja, K.U., Uthman, O., Vasankari, T., Vlassov, V., Vollset, S.E., Wakayo, T., Watkins, D., Weintraub, R., Werdecker, A., Westerman, R., Wiysonge, C.S., Wolfe, C., Workicho, A., Xu, G., Yano, Y., yip, P., Yonemoto, N., Younis, M., Yu, C., Vos, T., Naghavi, M., dan Murray, C. 2017. Global, regional, and national burden of cardiovascular diseases for 10 causes, 1990 to 2015. Journal Of The American College of Cardiology .70(1):1-25.

Ruiz-Teran, F., and Owens, J. D. 1996. Chemical and enzymic changes during the fermentation of bacteria-free soya bean tempe. Journal of the Science of Food and Agriculture. 71(4):523-530.

Rusdah. 2016. Antioxidative peptides of tempe from Indonesia. (Thesis). Institut Pertanian

Bogor, Bogor. Sangole, N. V., and Dadkar, V N. 2010. Adverse drug reaction monitoring with angiotensin converting enzyme inhibitors: A prospective, randomized, open-label, comparative study. Indian Journal of Pharmacology. 42(1): 27-31.

Sine, Y. 2016. Fermentasi tempe gude (Cajanus cajan L.) dan kualitas tempe hasil aktivitas jamur inokulum tradisional. (Thesis). Universitas Gadjah Mada, Yogyakarta.

Sparringa, R.A., dan Owens, J.D. 1999. Protein utilization during soybean tempe fermentation. Journal of Agricultural and Food Chemistry.47:4375-4378.

Starzynska-Janiszewska, A., Stodolak, B., and Wikiera, A. (2015). Proteolysis in tempeh-type products obtained with Rhizopus and Aspergillus strains from grass pea (Lathyrus sativus) seeds. Acta Science Polonorum Technologia Alimentaria.14(2):125–132.

Su, J. B. 2014. Different cross-talk sites between the renin−angiotensin and the kallikrein−kinin systems. Journal of the Renin-Angiotensin-Aldosterone. 15(4):319-328.



DOI: https://doi.org/10.22146/ifnp.46921

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Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

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