The Role of Extrinsic Pathway (Death Receptor Pathway) Apoptosis through Caspase-8 in Atherogenesis due to High Fat and High Cholesterol Diet

https://doi.org/10.22146/jifnp.33

Yanuartono Yanuartono(1*), Hastari Wuryastuti(2), R Wasito(3), Sri Raharjo(4)

(1) Gadjah Mada University
(2) Gadjah Mada University
(3) Gadjah Mada University
(4) Gadjah Mada University
(*) Corresponding Author

Abstract


Thirty male rats, strain Sprague Dawley were used as experimental animal to study the role of death receptor pathway apoptosis in atherogenesis due to high fat and high cholesterol diet. The rats were randomly alloted into three group (I, II, III) of 10 each. Group I as control was fed normal diet, group II was fed diet containing high fat diet, and group III was fed containing high fat and high cholesterol diet (atherogenic diet). After 6 and 12 weeks on experimental diet, 15 rats were selected randomly (5 rats of each group). All animal were then killed and the aorta were taken out for caspase-8 immunohistochemical analysis. Based on the present study result it can be concluded that high cholesterol and/or high fat diet induced apoptosis through death receptor pathway via caspase-8.

Keywords


Sprague Dawley; apoptosis; caspase-8; death receptor

Full Text:

PDF


References

Arbustini, E., Grasso, M., Diegoli, M., Pucci, A., Bramerio, M., Ardissino D., Angoli, L., de Seriv, S., Bramucci, E., Mussini, A., Minizioni, G., Vigano, M., and SPecchia, G., 1991. Coronary atherosclerotic plaques with and without thrombus in ischemic heart syndromes: a morphologic, immunohistochemical, and biochemical study. Am. J. Cardiol. 68: 36B-50B

Bennet M. R., Gibson D. F., Schwartz, S. M., and Tait J. F. 1995. Binding and phagocytosis of apoptotic vascular smooth muscle cells is mediated in part by exposure of phosphatidylserine. Circ. Res. 77: 1136-1142

Bennet, M. R., and Boyle, J. J. 1998. Apoptosis of vascular smooth muscle cells in atherosclerosis. Atherosclerosis 138: 3-9

Bennet, M. R., Evan, G. I., and Schwartsz, S. M. 1995. Apoptosis of human vascular smooth muscle cells derived from normal vessels and coronary atherosclerotic plaques. J. Clin. Invest, 95: 2266-2274

Best, P. J. M. Hasdai, D., Sangiorgi, G., Schwartz, R. S., Holmes, D. R. Jr., Simari, R. D., and Lerman A. 1999. Apoptosis. Basic concepts and implication in coronary artery disease. Aterioscler. Thromb. Vasc. Biol. 19: 14-22

Bjorkerud, S., and Bjorkerud, B. 1996. Apoptosis is abundant in human atherosclerotic lesions, especially in inflammatory cells (macrophages and T cells), and may contribute to accumulation of gruel and plaque instability. Am. J. Pathol. 149:367-280

Boldin, M. P., Goncharov, T. M., Goltsev, Y. V., and Wallach D. 1996. Involvement of MACH, a novel MORTI/FDD-interacting protease, in Fas/APO-1-and TNF-receptor induced cell death. Cell 85: 803-815

Cai W. J. Devaux, B., Schaper, W., and Schaper, J. 1997. The Role of Fas/APO 1 Apoptosis in the Development of Human Atherosclerotic Lesions. Atherosclerosis. 131: 177-186

Chang, H. Y., and Yang, X. 2000. Proteases for cell suicide: functions and regulation of caspases. Microbiol. And Mol. Biol. Rev. 64: 8212-846

Chaudhary, P. M., Eby, M. T., Jasmin, A., and Hood L.1999. Activation of the c-Jun N-terminal Kinase/Stress-activated Protein Kinase pathway by overexpression of caspase-8 and its homologs. J. Biol. Chem. 274: 19211-19219.

Chainnaiyan, A. M., O'Rourke,K. Tewari, M., and Dixit, V. M. 1995. FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis. Cell. 81: 505-512

Chisolm, G. M., Ma. G., Irwin, K C., Martin, L. L., GUnderson K. G., Linberg, L. F., Morel, D. W. and DiCorleto, P. E. 1994. 7β-Hydroxycholest5en-3β-ol, a Component of Human Atherosclerotic Lesions, is a Primary Cytotoxin of Oxidized Huma Low Density Lipoprotein. Proc. Natl. Acad. Sci. USA. 91:11452-11456

De Pablo, M. A., Susin, S. A., Jacotot, E., Larochette, N., Constatini, P. Ravagnan, L. Anami S. and Koremer, G. 1999. Palmitate Induces Apoptosis via Diet Effect on Mitochondria. Apoptosis 4:81-87

DeBlois, D., Tea, B. S., Than V. D., Tremblay, J., and Hamlet, P. 1997. Smooth muscle apoptosis during vascular regression in spontaneously hypertensive rats. Hypertension. 29: 340-349.

Duke R. C., Ojcius D. M., and Young J. D. 1996. Cell suicide in health and disease. Sci. Am. 275: 80-87

Garrat, K. N., Edwards, W. D., Kaufmann, U. P., Vlietsra, R. E., and Holmes, D. R. J. 1991. Differential Histopathology of primary atherosclerotic and restenotic lesions in coronary arteries and saphenous vein bypass grafts: analysis of tissue obtained from 73 patients by directional atherectomy. J. Am. Coll. Cardiol. 17:442-448

Geng Y., and Libby P. 1995. Evidence for apoptosis in advanced human antheroma: colocalization with interleukin-1a converting enzyme. Am. J. Pathol. 147: 251-266

Geng Y. J. Henderson, L. E., Levesque, E., B., Muszynski, M., and Libby P. 1997. Fas is expressed in human atherosclerotic intima and promotes apoptosis of cytokine-primed human vascular smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 17:2200-2208

Geng Y. J., Liao, H. S. and Mangovern, J. 1998. Expression of Fas Ligand in Advanced Human Atherosclerotic Lesions: Implication for Co-occurrence of Immunocytotoxicity and Immune Privilege. Circulation 98 (suppl) 1-48

Gibbon, G. H., and Dzau V. J. 1994. The emerging concept of vascular remodelling. N. Engl. J. Med. 330: 1431-1438

Han D. K. M., Haudenschild C. C., Hing M. K., Tinkle B. T., Leon M. B., and Liau G. 1995. Evidence for apoptosis in human atherosclerosis and rat vascular injury model. Am. J. Pathol. 197: 267-277

Harada, K., Ishibashi, S., Miyashita, T., Osuga, J., Yagyu, H., Ohashi, K., Yazaki, K., and Yamada N. 1997. Bcl-2 Protein Inhibits Oxysterol-Induced Apoptosis Through Suppressing CPP-32 Mediated Pathway. FEBS lett. 411:63-66

Harada-Shiba, M., Kinoshita, M., Kamido, H. and Shimokado, K. 1998. Oxidized Low Density Lipoprotein Induces Apoptosis in Cultures Human Umbilical Vein Endolethial Cells by Common and Unique Mechanisms. J. Biol. Chem. 273:9681-9687

Isner, J. M., Kearney, M., Bortman, S., and Passer, J. 1995. Apoptosis in human atherosclerosis and restenosis. Circulation 91: 2703-2711

Kellner-Weibel, G., Geng Y. J., and Rothblat, G. H. 1999. Cytotoxic Cholesterol in Generated by the Hydrolosis of Cytoplasmic Cholesteryl Ester and Transported to the Plasma Membrane. Atherosclerosis. 146: 309-319

Kockx M. M., DeMeyer, G. R., Muhring J., Bult, H., Bultnick, J., and Herman A. G. 1996. Apoptosis and Related Proteins in Different Stages of Human Atherosclerosis Plaques. Circulation. 97: 2307-2315.

Lee, T. S. and Chau, L. Y. 2001. Fas/Fas Ligand-Mediated Death Pathway is involved in Ox-LDL Induced Apoptosis in Vascular Smooth Muscle Cells. AM. J. Physiol. 280: C709-C718

Leonarduzzi, G., Sottero, B., and Poli, G., 2002, Oxidised Products of Cholesterol: Dietary and Metabolic Origin, and Proatherosclerotic Effects (Review). J. Nutr. Biochem. 13: 700-710

Libby, P., Geng Y. J., Aikawa M., Schoenbeck U., Mach F., Clinton, S. K., Sukhova G. K., and Lee R. T. 1996. Macrophages and atherosclerotic plaque stability. Curr. Opin. lipidol. 7: 330-335

Lutgens, E., Daemen, M., Kockx, M. Doesvendans, P., Hofker, M., Havekes, I., Wellens, H., and deMuinck E. D. 1999. Atherosclerotic in APOE*3-Leiden Transgenic Mice: from Proliferative to Athermatous Stage. Circulation. 99:276-283

Mallat, Z., and Tefgui, A. 2000. Apoptosis in the vasculature: mechanisms and functional importance. Br. J. Pharmacol. 130: 947-962

Micheau, O. and Tschopp, J. 2003. Induction of TNF Receptor I-Mediated Apoptosis via Two Sequential Signaling Complexes. Cell. 114: 181-190

Miguet-Alfonsi, C., Prunet, C. and Monier, S. 2002. Analysis of Oxidative Processes and of Myelin Figure Formation Before and After the Loss of Mitochondria Transmembrane Potential During 7a-Hydrixycholesterol (7a-OH) and 7-Ketocholesterol-Induced Apoptosis: Comparison with Various Pro-Apoptotic Chemicals. Biochem. Pharmacol. 64: 527-541

Nicholson D. W. 1999. Caspase structure, proteolytic, substrates, and function during apoptotic cell death. Cell death differ. 6: 1028-1042

Schneider, D. B., Vassalli, G., Wen, S., Driscoll, R. M., Sassani, A. B., DeYoung, M. B., Linnemann, R., Virmain, R. and DIchek, D. A. 2000. Expression of Fas Ligand in Arteries of Hypercholesterolemic Rabbits Accelerates Atherosclerotic Lesion Formation. Arterioscler. Thromb. Vasc. Biol. 20: 298-308

Sevanian, A., Hodis, H. N., Hwang J., McLeod, L. L. and Peterson, H. 1995. Characterization of Endothelial Cell Injury by Cholesterol Oxidation Products Found in Oxidized LDL. J. Lipid Res. 36: 1971-1986

Seye, C. I., Knaapen, M. W. M., and Daret, D. 2004. 7-Ketochoresterol Induces Reversible Cytochrome c Release in Smooth Muscle Cells in Absence of Mitochondrial Swelling. Cardiovasc. Res. 64: 144-153

Thomas, W. A., Reiner, J. M., FLorentin, F. A., Lee, K. T., Lee, W. M. 1976. Population dynamics of arterial smooth muscle cells, V: cell proliferation and cell death during initial 3 months in atherosclerotic lesions induce in swine by hypercholesterolemia diet and intimal trauma Exp. Mol. Pathol. 24: 360-374

Thompson, C. B. 1995. Apoptosis in the pathogenesis and treatment and disease. Science 2267: 1456-1462.

Trieb, K., Cetin, E., Girsch, W., and Brand, G. 2003. Distinct expression of Apo-1 and caspase-8 in human growth plate. J. European Cells and Materials. 5: suppl 57-58

Varfolomeev, E.E. and Ashkenazi, A. 2004. TUmor Necrosis Factor: an Apoptosis JuNKie ?. Cell 116: 491-497

Wasito, R. 1997. Immunocytochemistry in diagnostic pathology: Use of immunohistochemical techniques for detecting porcine specific RNA transmissible gastroenteritis virus in vivo. Indon J. Biotech. June: 121-124

William, G. T. 1991. Programmed cell death: apoptosis and oncogenesis. Cell 65: 1097-1098.

Wyllie, A. H., Kerr, J. F.R., Currie, A. R. 1980. Cell death:the significanceof apoptosis. Int. Rev. Cytol. 68: 251-306

Yuan X. M. 1999. Apoptotic macrophage-derived foam cells of human atheromas are rich in iron and ferritin suggesting iron-catalyzed reactions to be involved in apoptosis. Free Radic. Res. 30: 221-231



DOI: https://doi.org/10.22146/jifnp.33

Article Metrics

Abstract views : 979 | views : 706

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

Journal of Indonesian Food and Nutrition Progress have been indexed by: 

   

 

This works is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.