* Corresponding Author
Polymorphism and Association of the Novel KCTD2 Gene with Flavor and Odor in Indonesian Local Sheeps
https://doi.org/10.21059/buletinpeternak.v47i1.80711 Herpina Herpina(1), Kasita Listyarini(2), Cece Sumantri(3), Katrin Roosita(4), Asep Gunawan(5*)
(1) Graduate School of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia
(2) Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia
(3) Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia
(4) Department of Community Nutrition, Faculty of Human Ecology, IPB University, Bogor, 16680, Indonesia
(5) Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia
(*) Corresponding Author
Abstract
A candidate marker that influences the flavor and odor of Indonesian sheep that is the Potassium Channel Tetramerization Domain Containing 2 (KCTD2) gene. This study aims to examine the polymorphism and association between the KCTD2 gene and lamb flavor and odor in Indonesian local sheeps. This study used DNA taken from the longissimus dorsi (LD) muscle of 100 rams including 75 Javanese Thin-Tail sheep (JTTS), 10 Javanese Fat-Tail Sheep (JFTS), and 15 Jonggol Sheep (JS), with ranged in weight from 20 to 35 kg and were 10 to 12 months old. The PCR-RFLP technique and GLM test analysis were used to identify polymorphisms and association of KCTD2 gene. The restriction enzyme BafI digests the PCR-RFLP product of the KCTD2 gene. The results showed that the KCTD2 gene was polymorphic (CC and CT). KCTD2 gene analysis showed a significant (P<0.05) association with 3-Methylphenol (MP). This research provide information regarding the role of the KCTD2 gene in lamb flavor and odor, especially in 3-Methylphenol (MP) compounds, and explain KCTD2 as a functional gene in the selection of premium sheep with low flavor and odor in lamb meat.
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Angrisani, A., A. D. Fiore, and E. D. Smaele. 2021. The emerging role of the KCTD proteins in cancer. Cell Commun. Signal. 19: 56.
Bardakci, F. 2001. Random amplified polymorphic DNA (RAPD) markers. Turk. J. Biol. 25: 185-196.
[BPS] Central Bureau of Statistics. 2020. Lamb meat production by province 2018-2020. Central Bureau of Statistics, Jakarta.
Boada, M., C. Antunez, and R. R. Lorca. 2014. ATP5H/KCTD2 locus is associated with alzheimer's disease risk. Mol Psychiatry. 19: 682-687.
Brunton, N. P., D. A. Cronin, and F. J. Monahan. 2002. Volatile components associated with freshly cooked and oxidized off-flavours in turkey breast meat. Flavour Fragr. J. 17: 327-334.
Edey, T., N. 1983. The genetic pool of sheep and goats. Tropical sheep and goat production. Canberra. Australia: Australian Universities International Development Program (AUIDP). p. 3-5.
Falahudin, A., O. Imanudin. 2018. Quality of lamb meat feeding vegetable waste silage. Integrated Animal Husbandry Scientific Journal 6: 140-146.
Gunawan, A., S. Sahadevan, M. U. Cinar, C. Neuhoff, C. Große-Brinkhaus, L. Frieden, D. Tesfaye, E. Tholen, C. Looft, D. S. Wondim, M. Hölker, and M. J. Uddin. 2013. Identification of the novel candidate genes and variants in boar liver tissues with divergent skatole levels using RNA deep sequencing. PLoS One. 8(8).p.e72298.
Gunawan, A., S. Tazkya, K. Listyarini, M. Yamin, I. Inounu, and C. Sumantri. 2018a. Characterization of the KIF12 gene (Kinesin Family 12) and it’s the relationship with fatty acid composition in sheep. Jurnal Ilmu dan Teknologi Peternakan Tropis 5: 88-94.
Gunawan, A., Jakaria, K. Listyarini, A. Furqon, C. Sumantri, S. H. Akter, and M. J. Uddin. 2018b. Transcriptome signature of liver tissue with divergent sheepmeat odour and flavour using RNA deep sequencing. Gene. 676: 86-94
Harahap, R. S., R. R. Noor, and A. Gunawan. 2021. Polymorphism and expression of the HSD17β13 gene and its association with Indonesian sheep quality. Animal Production 23: 44-53.
Jarmuji, J. 2014. Lactation curve of Jonggol ewes (thin tail sheep) on ewe age were reared grazing system. Jurnal Sain Peternakan Indonesia 9: 51-59.
Jayasena, D. D, U. A. Dong C. N. Ki, and J. Cheorun. 2013. Flavour chemistry of chicken meat; A review. Asian-Australas J. Anim. Sci. 26: 732-742.
Khasanah, H., A. Gunawan, R. Priyanto, M. F. Ulum, and Jakaria. 2016. Polymorphism of myostatin (MSTN) promoter gene and its association with growth and muscling traits in bali cattle. Med. Pet. 39: 95-103.
Listyarini, K., C. Sumantri, S. Rahayu, M. J. Uddin, and A. Gunawan. 2022. Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep. Anim. Biosci. 35: 1489-1498.
Listyarini, K., Jakaria, M. J. Uddin, C. Sumantri, and A. Gunawan. 2018. Association and expression of CYP2A6 and KIF12 genes related to lamb flavor and odor. Trop. Anim. Sci. J. 41: 100-107.
Nei, M., S. Kumar. 2000. Molecular evolution and phylogenetics. NY, USA, Oxford University Press.
Reinecceius, G. A. 1997. Source Book of Flavor. 2 nd ed. New York: Chapmad and Hall.
Sodiq, A., and E. S. Tawfik. 2004. Productivity and breeding strategies of sheep in Indonesia: a review. J. Agric. Rural Dev. Trop. Subtrop. 105: 71-82.
Strathe, A. B., I. H. Velander, T. Mark, and H. N. Kadarmideen. 2013. Genetic parameters for androstenone and skatole as indicators of boar taint and their relationship to production and litter size traits in danish landrace. Anim. Sci. J. 91: 2587-2595.
Teng, X., A. Aouacheria , L. Lionnard, K. A. Metz, L. Soane, A. Kamiya, and J. M. Hardwick. 2019. KCTD: A new gene family involved in neurodevelopmental and neuropsychiatric disorders. CNS Neurosci Ther. 25: 887-902.
Watkins, P. J., G. Kearney, G. Rose, D. Allen, A. J. D. Ball, W. Pethick, and R. D. Warner. 2014. Effect of branched-chain fatty acids 3-methylindole and 4-methylphenol on consumer sensory scores of grilled lamb meat. Meat Sci. 96:1088-109.
Wasserman, A. E. 1972. Thermally produced flavor components in the aroma of meat and poultry. J. Agric. Food Chem. 20: 737-741.
DOI: https://doi.org/10.21059/buletinpeternak.v47i1.80711
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