Embryo Development and Chick Performance of Local Chicken Following In-Ovo Injection of L-Arginine Into Local Chicken Eggs

Muhammad Taufik; Urfiana Sara; Muhammad Yusuf; Fatma Maruddin; Muhammad Azhar

doi:10.21059/buletinpeternak.v47i2.80642

Embryo Development and Chick Performance of Local Chicken Following In-Ovo Injection of L-Arginine Into Local Chicken Eggs

https://doi.org/10.21059/buletinpeternak.v47i2.80642

Muhammad Taufik⁽¹⁾, Urfiana Sara⁽²⁾, Muhammad Yusuf⁽³⁾, Fatma Maruddin⁽⁴⁾, Muhammad Azhar^(5*)

(1) Department of Animal Agriculture, Politeknik Pembangunan Pertanian Gowa, Gowa, 92171
(2) Department of Animal Agriculture, Politeknik Pembangunan Pertanian Gowa, Gowa, 92171
(3) Department of Animal Agriculture, Politeknik Pembangunan Pertanian Gowa, Gowa, 92171
(4) Department of Animal Agriculture, Hasanuddin University, Makassar, 90245
(5) Department of Animal Agriculture, Politeknik Pembangunan Pertanian Gowa, Gowa, 92171
(*) Corresponding Author

Abstract

This study aims to determine the effect of in-ovo injection of L-arginine into local chicken eggs on embryo development and chick performance of local chicken. A total of 160 eggs were incubated using semi-automatic incubator with temperature of 37-38C and relative humidity of 55-65%. The injected L-arginine solution has a concentration of 0.5% (m/v). In treatment was divided into 4 groups, the first treatment was without injection (control), the second treatment was the injection of 0.2 mL L-arginine solution 0.5% (m/v)/egg, the third treatment was the injection of 0.4 mL L-arginine solution. 0.5% (m/v)/egg, and fourth treatment was the injection of L-arginine solution 0.6 mL 0.5%(m/v)/egg. The results showed that embryo mortality and hatchability were lower with L-arginine injection treatment than without injection. All treatments showed no effect on extraembryonic fluid absorption, but there was an increase in embryo weight in the injection treatment 0.2, 0.4, 0.6 mL L-Arginine 0.5% /eggs 2,355 g, 2,577 g, 2,705, respectively. In conclusion, an in-ovo injection of L-arginine in local chicken eggs has a good effect (the beneficial effect) on embryo death and embryo performance. Injection of 0.4 ml L-Arginine 0.5% /eggs improved hatchability and Newly Hatched Chick Weight (NHCW).

Keywords

Chick quality; embryo performance; hatchability; in-ovo injection; L-arginine

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References

Abousaad, S., K. Lassiter, A. Piekarski, P. Chary, K. Striplin, K. Christensen, L. R. Bielke, B. M. Hargis, S. Dridi, and W. G. Bottje. 2017a. Effect of in ovo feeding of dextrin- iodinated casein in broilers: II. Hatch window and growth performance. Poult. Sci. 96: 1478–1484. https://doi.org/10.3382/ps/pew439

Abousaad, S., K. Lassiter, A. Piekarski, P. Chary, K. Striplin, K. Christensen, L. R. Bielke, B. M. Hargis, S. Dridi, and W. G. Bottje. 2017b. Effects of in ovo feeding of dextrin- iodinated casein in broilers: I. Hatch weights and early growth performance. Poult. Sci. 96: 1473–1477. https://doi.org/10.3382/ps/pew438

Syahruddin, L. Agustina, W. Pakiding, and R. Malaka. 2018. Supplementation of L- Arginine through the parent feed and in ovo feeding on post-hatch performances of local ducks. I. J. Sci. Basic Appl. Res. 40: 87–98.

Al-Daraji, H. J., A. A. Al-Mashadani, W. K. Al- Hayani, A. S. Al-Hassani, and H. A. Mirza. 2012. Effect of in ovo injection with L- arginine on productive and physiological traits of Japanese quail. S. Afr. J. Anim. Sci. 42: 139–145. https://doi.org/10.4314/sajas.v42i2.6

Araújo, I. C. S., M. B. Café, M. A. Mesquita, B. N. Caiado, A. M. Faria, H. H. C. Mello, J. H.
Stringhini, and N. S. M. Leandro. 2020. Effect of a commercial product containing canthaxanthin for in ovo feeding to broiler embryos on hatchability, chick quality, oxidation status, and performance. Poult. Sci. 99: 5598–5606. https://doi.org/10.1016/ j.psj.2020.08.044

Araújo, I. C. S., M. B. Café, R. A. Noleto, J. M. S. Martins, C. J. Ulhoa, G. C. Guareshi, M. M. Reis, and N. S. M. Leandro. 2019. Effect of vitamin E in ovo feeding to broiler embryos on hatchability, chick quality, oxidative state, and performance. Poult. Sci. 98:3652–3661. https://doi.org/10.3382/ps/pey439.

Azhar, M., D. P. Rahardja, W. and Pakiding. 2016. Embryo development and post-hatch performances of kampung chicken by in ovo feeding of L-arginine. Media Peternakan 39: 168–172. https://doi.org/10.5398/medpet. 2016.39.3.168

Castañeda, C. D., J. N. Gamble, K. G. S. Wamsley, C. D. McDaniel, and A. S. Kiess.
2021. In ovo administration of Bacillus subtilis serotypes effect hatchability, 21- day performance, and intestinal microflora. Poult. Sci. 100 (6). https://doi.org/10.1016/j. psj.2021.101125

Daneshyar, M., J. M. C. Geuns, J. G. Buyse, H. Kermanshahi, H. Willemsen, Z. Ansari, E. Decuypere, and N. Everaert. 2010. Evaluation of steviol injection on chicken embryo: effect on post-hatch development, proportional organ weight, plasma thyroid hormone and metabolites. J. Poult. Sci. 47: 71-76. http://dx.doi.org/10.2141/jpsa.009059

Fatemi, S. A., A. H. Alqhtani, K. E. C. Elliott, A. Bello, A. W. Levy, and E. D. Peebles.
2021a. Improvement in the performance and inflammatory reaction of Ross 708 broilers in response to the in ovo injection of 25-hydroxyvitamin D3. Poult. Sci. 100: 138–146. https://doi.org/10.1016/j.psj.2020. 10.010

Fatemi, S. A., K. E. C. Elliott, A. Bello, H. Zhang, A. Alqhtani, and E. D. Peebles. 2021b.
Effects of the in ovo injection of vitamin D3 and 25-hydroxyvitamin D3 in Ross 708 broilers subsequently fed commercial or calcium and phosphorus-restricted diets. II. Immunity and small intestine morphology 1, 2, 3. Poult. Sci. 100: 101240. https://doi.org/10.1016/j.psj.2021.101240

Firman, C. A. B., V. Inhuber, D. J. Cadogan, W. H. E. J. van Wettere, and R. E. A. Forder.
2023. Effect of in ovo creatine monohydrate on hatchability, post-hatch performance, breast muscle yield and fiber size in chicks from young breeder flocks.
Poult. Sci. 102: https://doi.org/10.1016/j.psj.2022.102447

Fouad, A. M., H. K. El-Senousey, X. J. Yang, and J. H. Yao. 2012. Role of dietary L-arginine in poultry production. I. J. Poult. Sci. 11: 718–729. https://doi.org/10.3923/ijps.2012.718.729

Foye, O. T., P. R. Ferket, and Z. Uni. 2007. The effects of in ovo feeding arginine, β-
hydroxy-β-methyl- butyrate, and protein on jejunal digestive and absorptive activity in
embryonic and neonatal turkey poults. Poult. Sci. 86: 2343–2349.
https://doi.org/10.3382/ps.2007-00110

Foye, O. T., Z. Uni, J. P. McMurtry, and P. R. Ferket. 2006. The effects of amniotic nutrient administration, “in-ovo feeding” of arginine and/or ß-hydroxy-ß-methyl butyrate (HMB) on insulin-like growth factors, energy metabolism and growth in turkey poults. I. J. Poult. Sci. 5: 309-317.

Givisiez, P. E. N., A. L. B. Moreira Filho, M. R. B. Santos, H. B. Oliveira, P. R. Ferket, C. J. B. Oliveira, and R. D. Malheiros. 2020. Chicken embryo development: metabolic and morphological basis for in ovo feeding technology. Poult. Sci. 99: 6774–6782. https://doi.org/10.1016/j.psj.2020.09.074

Han, G., Y. Ouchi, T. Hirota, S. Haraguchi, T. Miyazaki, T. Arakawa, N. Masuhara, W. Mizunoya, R. Tatsumi, K. Tashiro, T. Bungo, M. Furuse, and V. S. Chowdhury. 2020. Effects of l-leucine in ovo feeding on thermotolerance, growth and amino acid metabolism under heat stress in broilers. Animal 14: 1701–1709. https://doi.org/10.1017/S1751731120000464

Kim, D.-H., J. Lee, Y. M. Choi, and K. Lee. 2023. Research Note: Growth promoting potential in Mstn mutant quail dependent and independent of increased egg size. Poult. Sci. 102: 102260. https://doi.org/10.1016/j.psj.2022.102260

Kim, E., N. Akhtar, J. Li, Q. Hui, B. Dong, C. Yang, and E. G. Kiarie. 2020. In ovo feeding of epidermal growth factor: embryonic expression of intestinal epidermal growth factor receptor and posthatch growth performance and intestinal development in broiler chickens. Poult. Sci. 99: 5736–5743. https://doi.org/10.1016/j.psj.2020.07.029

Kornasio, R., O. Halevy, O. Kedar, and Z. Uni. 2011. Effect of in ovo feeding and its interaction with timing of first feed on glycogen reserves, muscle growth, and body weight. Poult. Sci. 90: 1467–1477. https://doi.org/10.3382/ps.2010-01080

Li, T., C. D. Castañeda, J. Miotto, C. McDaniel, A. S. Kiess, and L. Zhang. 2021. Effects of in ovo probiotic administration on the incidence of avian pathogenic Escherichia coli in broilers and an evaluation on its virulence and antimicrobial resistance properties. Poult. Sci. 100: 1–12. https://doi.org/10.1016/j.psj.2020.11.072

Miri, B., H. A. Ghasemi, I. Hajkhodadadi, and A. H. K. Farahani. 2022. Effects of low eggshell temperatures during incubation, in ovo feeding of L-arginine, and post-hatch dietary guanidinoacetic acid on hatching traits, performance, and physiological responses of broilers reared at low ambient temperature. Poult. Sci. 101: https://doi.org/10.1016/j.psj.2021.101548

Murakami, A. E., J. I. M. Fernandes, L. Hernandes, and T. C. Santos. 2012. Effects of starter diet supplementation with arginine on broiler production performance and on small intestine morphometry. Pesq. Vet. Bras. 32: 259–266. https://doi.org/10.1590/S0100-736X2012000300014

Omede, A., M. M. Bhuiyan, A. F. Lslam, and P. A. Iji. 2017. Physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed supplement. Asian-Australas J. Anim. Sci. 30: 1124–1134. https://doi.org/10.5713/ajas.16.0677

Padhi, M. K. 2016. Importance of Indigenous Breeds of Chicken for Rural Economy and Their Improvements for Higher Production Performance. Scientifica, 2016. https://doi.org/10.1155/2016/2604685

Peebles, E. D., T. F. B. Oliveira, E. J. Kim, O. C. Olojede, K. E. C. Elliott, L. L. Lindsey, and P. D. Gerard. 2021. Research Note: Effects of the in ovo injection of organic zinc, manganese, and copper and posthatch holding time before placement on broiler body temperature during grow out. Poult. Sci. 100: 755–759. https://doi.org/10.1016/j.psj.2020.10.048

Rahardja, D. P., M. R. Hakim, and V. S. Lestari. 2018. Application of in ovo injection of L-Glutamine for improving productivity of Indonesian native chicken: Hatchability and hatching time. IOP Conf. Ser.: Earth Environ. Sci. 157(1). https://doi.org/10.1088/1755-1315/157/1/012071

Rahardja, D. P., M. R. Hakim, M. Yusuf, and V. S. Lestari. 2019. Hatching and post-hatching performances of Indonesian native chicken eggs infused saline solution. IOP Conf. Ser.: Earth Environ. Sci. 247(1). https://doi.org/10.1088/1755-1315/247/1/012014

Subramaniyan, S. A., D. R. Kang, J. R. Park, S. H. Siddiqui, P. Ravichandiran, C. S. Na, and K. S. Shim. 2019. Effect of in ovo injection of l-arginine in different chicken embryonic development stages on post-hatchability, immune response, and myo-d and myogenin proteins. Anim. 357: 1-18.

Shafey, T. M., M. A. Alodan, I. M. Al-Ruqaie, and M. A. Abouheif. 2012. In ovo feeding of carbohydrates and incubated at a high incubation temperature on hatchability and glycogen status of chicks. S. Afr. J. Anim. Sci. 42: 210–220. https://doi.org/10.4314/sajas.v42i3.2

Silva, L. M. G. S., A. E. Murakami, J. I. M. Fernandes, R. D. Dalla, and J. F. Urgnani. 2012. Effects of dietary arginine supplementation on broiler breeder egg production and hatchability. Brazilian J. Poult. Sci. 14: 267–273. https://doi.org/10.1590/S1516-635X2012000400006

Slawinska, A., M. Zampiga, F. Sirri, A. Meluzzi, M. Bertocchi, S. Tavaniello, and G. Maiorano. 2020. Impact of galactooligosaccharides delivered in ovo on mitigating negative effects of heat stress on performance and welfare of broilers. Poult. Sci. 99: 407–415. https://doi.org/10.3382/ps/pez512

Soliman, M. A., M. H. Khalil, K. El-Sabrout, and M. K. Shebl. 2020. Crossing effect for improving egg production traits in chickens involving local and commercial strains. Vet. World, 13: 407–412. https://doi.org/10.14202/vetworld.2020.407-412

Tong, Q., C. E. Romanini, V. Exadaktylos, C. Bahr, D. Berckmans, H. Bergoug, N. Eterradossi, N. Roulston, R. Verhelst, I. M. McGonnell, and T. Demmers. 2013. Embryonic development and the physiological factors that coordinate hatching in domestic chickens. Poult. Sci. 92: 620–628. https://doi.org/10.3382/ps.2012-02509

Wang, J., J. Lin, J. Wang, S. Wu, G. Qi, H. Zhang, and Z. Song. 2020. Effects of in ovo feeding of N-acetyl-L-glutamate on early intestinal development and growth performance in broiler chickens. Poult. Sci. 99: 3583–3593. https://doi.org/10.1016/j.psj.2020.04.003

Yu, L. L., T. Gao, M. M. Zhao, P. A. Lv, L. Zhang, J. L. Li, Y. Jiang, F. Gao, and G. H. Zhou. 2018a. Effects of in ovo feeding of l-arginine on breast muscle growth and protein deposition in post-hatch broilers. Anim. 12: 2256–2263. https://doi.org/10.1017/S1751731118000241

Yu, L. L., T. Gao, M. M. Zhao, P. A. Lv, L. Zhang, J. L. Li, Y. Jiang, F. Gao, and G. H. Zhou. 2018b. In ovo feeding of L-arginine alters energy metabolism in post-hatch broilers. Poult. Sci. 97: 140–148. https://doi.org/10.3382/ps/pex272

Zhang, J., K. Cai, R. Mishra, and R. Jha. 2020. In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens. Poult. Sci. 99: 4776–4785. https://doi.org/10.1016/j.psj.2020.06.061

Zhang, X. Y., L. L. Li, L. P. Miao, N. N. Zhang, and X. T. Zou. 2018. Effects of in ovo feeding of cationic amino acids on hatchability, hatch weights, and organ developments in domestic pigeon squabs (Columba livia). Poult. Sci. 97: 110–117. https://doi.org/10.3382/ps/pex260

Zhao, M. M., T. Gao, L. Zhang, J. L. Li, P. A. Lv, L. L. Yu, F. Gao, and G. H. Zhou. 2017a. Effects of in ovo feeding of creatine pyruvate on the hatchability, growth performance and energy status in embryos and broiler chickens. Anim. 11: 1689–1697. https://doi.org/10.1017/S1751731117000374

Zhao, M. M., T. Gao, L. Zhang, J. L. Li, P. A. Lv, L. L. Yu, F. Gao, and G. H. Zhou. 2017b. In ovo feeding of creatine pyruvate alters energy reserves, satellite cell mitotic activity and myogenic gene expression of breast muscle in embryos and neonatal broilers. Poult. Sci. 96: 3314–3323. https://doi.org/10.3382/ps/pex150

Zhu, M. K., X. Y. Zhang, X. Y. Dong, and X. T. Zou. 2019a. Effects of in ovo feeding of L-lysine on hatchability, hatching time, and early post-hatch development in domestic pigeons (Columba livia). Poult. Sci. 98: 5533–5540. https://doi.org/10.3382/ps/pez300

Zhu, Y. F., M. B. Bodinga, J. H. Zhou, L. Q. Zhu, Y. L. Cao, Z. Z. Ren, and X. J. Yang. 2020. Effects of in ovo injection of vitamin C on heat shock protein and metabolic genes expression. Anim. 14: 360–367. https://doi.org/10.1017/S1751731119002088

Zhu, Y. F., S. Z. Li, Q. Z. Sun, and X. J. Yang. 2019b. Effect of in ovo feeding of Vitamin C on antioxidation and immune function of broiler chickens. Anim. 13: 1927–1933. https://doi.org/10.1017/S1751731118003531

DOI: https://doi.org/10.21059/buletinpeternak.v47i2.80642