POTENSI DARI KAPANG Aspergilus niger, Rhizophus oryzae DAN Neurospora sitophila SEBAGAI PENGHASIL EZIM FITASE DAN AMILASE PADA SUBSTRATE AMPAS TAHU

Atit - Kanti

doi:10.21059/buletinpeternak.v41i1.13337

POTENSI DARI KAPANG Aspergilus niger, Rhizophus oryzae DAN Neurospora sitophila SEBAGAI PENGHASIL EZIM FITASE DAN AMILASE PADA SUBSTRATE AMPAS TAHU

https://doi.org/10.21059/buletinpeternak.v41i1.13337

Atit - Kanti^(1*)

(1) Research Center for Biology-LIPI
(*) Corresponding Author

Abstract

Penambahan enzim hidrolisis untuk pakan ternak dapat meningkatkan nilai nutrisi pakan. Penelitian bertujuan untuk mendapatkan kondisi optimal untuk produksi enzim amilase dan fitase pada media ampas tahu menggunakan Aspergilus niger, Rhizophus oryzae dan Neurospora sitophila. Uji kemampuan produksi enzim fitase dan amilase oleh Aspergilus niger, Rhizophus oryzae dan Neurospora sitophila dilakukan menggunakan media ampas tahu yang disterilisasi. Pemilihan ketiga isolat ini diawali dengan uji produksi enzim amilase pada kultur cair yang mengandung 2 % pati, dan uji fitase dilakukan pada media yang mengandung 0.5 % sodium fitat. Hasil uji pada medium cair selanjutnya digunakan untuk uji produksi enzim fitase dan fitase pada sistem fermentasi padat (SSF) menggunakan ampas tahu sebagai media fermentasi. Untuk mendapatkan produksi enzim yang tinggi dilakukan melalui optimasi waktu inkubasi, suhu inkubasi dan pH media. Fitase dan amilase dapat diproduksi dengan media ampas tahu oleh R. oryzae, A. niger dan N. sitophila. Kondisi optimum untuk produksi fitase, yaitu waktu inkubasi pada hari keempat untuk ketiga kapang, suhu 25 °C untuk R. oryzae dan A. niger, suhu 30°C untuk N. sitophila, pH 8 untuk R. oryzae, pH 6 untuk Aspergillus niger dan N. Sitophila. Neurospora sitophila menghasilkan amilase optimum pada suhu 35°C, sedangkan Aspergillus niger dan Rhizopus oryzae optimum pada suhu 30°C. Penurunan aktivitas produksi amilase menurun oleh R. oryzae pada suhu 40°C. Amilase diproduksi optimal pada pH 6-7. Pakan ternak yang mengandung asam fitat mampu dihidrolisis oleh fitase pada kondisi optimum. Ketiga kapang juga menghasilkan enzim amilase pada media ampas tahu mengindikasikan bahwa ampas tahu merupakan susbtrat yang baik untuk produksi enzim hidrolisis yang berguna untuk meningkatkan nilai nutrisi pakan ternak.

(Kata kunci: Amilase, Aspergilus niger, Neurospora sitophila, phytase, Rhizophus oryzae )

Keywords

Kata kunci: Amilase, Aspergilus niger, Neurospora sitophila, phytase, Rhizophus oryzae

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References

Ahmad, T., S. Rasool, M. Sarwar, A. U. Haq, & Z. U. Hasan. 2000. Effect of microbial phytase produced from a fungus Aspergillus niger on bioavailability of phosphorus and calcium in broiler chickens. Animal Feed Science and Technology, 83(2), 103–114. http://doi.org/10.1016/S0377-8401(99)00122-4

Bogar, B., G. Szakacs, J. C. Linden, A. Pandey, & R. P. Tengerdy. 2003. Optimization of phytase production by solid substrate fermentation. Journal of Industrial Microbiology & Biotechnology, 30(3), 183–9. http://doi.org/10.1007/s10295-003-0027-3

Buckle, K. A. 1988. Characterization of extra- and intracellular phytases from Rhizopus oligosporus used in tempeh production. International Journal of Food Microbiology, 6(1), 67–79. http://doi.org/10.1016/0168-1605(88)90086-4

Davis, R. H., & D. D. Perkins. 2002. Timeline: Neurospora: a model of model microbes. Nature Reviews. Genetics, 3(May), 397–403. http://doi.org/10.1038/nrg797

Dersjant-Li, Y., A. Awati, H.Schulze, & G. Partridge. 2015. Phytase in non-ruminant animal nutrition: A critical review on phytase activities in the gastrointestinal tract and influencing factors. Journal of the Science of Food and Agriculture. http://doi.org/10.1002/jsfa.6998

Francis, F., A.Sabu, K. M. Nampoothiri, S.Ramachandran, S. Ghosh, G. Szakacs, & A.Pandey. 2003. Use of response surface methodology for optimizing process parameters for the production of alpha-amylase by Aspergillus oryzae. Biochemical Engineering Journal, 15(2), 107–115. http://doi.org/Pii S1369-703x(02)00192-4

Freitas, A., B. Escaramboni, A Carvalho, V. Lima, & P. Oliva-Neto. 2014. Production and application of amylases of Rhizopus oryzae and Rhizopus microsporus var. oligosporus from industrial waste in acquisition of glucose. Chemical Papers, 68(4), 442–450. http://doi.org/10.2478/s11696-013-0466-x

Gargova, S., Z. Roshkova, & G.Vancheva. 1997. Screening of fungi for phytase production. Biotechnology, 11(4), 221–224. http://doi.org/10.1023/A:1018426119073

Ghosh, K., K.Chakraborty, S. K. Sen, & A. K. Ray. 2001. Effects of thermostable bacterial α-amylase on growth and feed utilization in Rohu, Labeo rohita (Hamilton), fingerlings. Israeli Journal of Aquaculture - Bamidgeh, 53(3–4), 101–109.

Gracia, M. I., M. J. Araníbar, R. Lázaro, P. Medel, & G. G. Mateos. 2003. Alpha-amylase supplementation of broiler diets based on corn. Poultry Science, 82(3), 436–442. http://doi.org/10.1093/ps/82.3.436

Guo, M. J., Y. P. Zhuang, J. Chu, S. L. Zhang, A. S. Xiong, R. H. Peng, & Q. H. Yao, 2007. Production and purification of a novel thermostable phytase by Pichia pastoris FPHY34. Process Biochemistry, 42(12), 1660–1665. http://doi.org/10.1016/j.procbio.2007.09.003

Han, Y. W., D. J.Gallagher, & A. G. Wilfred. 1987. Phytase production by Aspergillus ficuum on semisolid substrate. Journal of Industrial Microbiology, 2(4), 195–200. http://doi.org/10.1007/BF01569540

Hang, H., X.Ye, M.Guo, J.Chu, Y. Zhuang, M. Zhang, & S. Zhang. 2009. A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate. Enzyme and Microbial Technology, 44(4), 185–188. http://doi.org/10.1016/j.enzmictec.2008.12.002

Kalsum, U., & O.Sjofjan. 2008. Pengaruh waktu inkubasi campuran ampas tahu dan onggok yang difermentasi dengan Neurospora sitophila terhadap kandungan zat makan. In Inovasi teknologi mendukung pengembangan agribisnis peternakan ramah lingkungan. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner (pp. 226–232).

Kumar, V., A. K. Sinha, H. P. S. Makkar, G. De Boeck, & K. Becker. 2012. Phytate and phytase in fish nutrition. Journal of Animal Physiology and Animal Nutrition. http://doi.org/10.1111/j.1439-0396.2011.01169.x

Li, S., D.Zhu, K. Li, Y. Yang, Z. Lei, & Z. Zhang. 2013. Soybean Curd Residue: Composition, Utilization, and Related Limiting Factors. ISRN Industrial Engineering, 2013, 1–8. http://doi.org/10.1155/2013/423590

Nakamura, Y., H. Fukuhara, & K. Sano. 2000. Secreted phytase activities of yeasts. Bioscience, Biotechnology, and Biochemistry, 64(4), 841–4. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10830502

Papagianni, M., S. E. Nokes, & K. Filer 2001. Submerged and Solid-State Phytase Fermentation by Aspergillus niger: Effects of Agitation and Medium Viscosity on Phytase Production, Fungal Morphology and Inoculum Performance. Food Technology and Biotechnology, 39(4), 319–326.

Quan, C. S., S.Fan, L. H. Zhang, Y. J. Wang, & Y.Ohta. 2002. Purification and properties of a phytase from Candida krusei WZ-001. Journal of Bioscience and Bioengineering, 94(5), 419–425. http://doi.org/10.1016/S1389-1723(02)80219-5

Ramachandran, S., K. Roopesh, & K. M.Nampoothiri. 2005. Mixed substrate fermentation for the production of phytase by Rhizopus spp . using oilcakes as substrates, 40, 1749–1754. http://doi.org/10.1016/j.procbio.2004.06.040

Rani, R., & S. Ghosh. 2011. Production of phytase under solid-state fermentation using Rhizopus oryzae: novel strain improvement approach and studies on purification and characterization. Bioresource Technology, 102(22), 10641–9. http://doi.org/10.1016/j.biortech.2011.08.075

Ray, R. C. 2004. Extracellular amylase(s) production by fungi Botryodiplodia theobromae and Rhizopus oryzae grown on cassava starch residue. Journal of Environmental Biology, 25(4), 489–495.

Sabu, A., S. Sarita, A. Pandey, B. Bogar, G. Szakacs, & C. R. Soccol. 2002. Solid-State Fermentation for Production of Phytase by Rhizopus oligosporus. Applied Biochemistry and Biotechnology, 102–103. http://doi.org/10.1385/ABAB:102-103:1-6:251

Sajjadi, M., & C. G. Carter. 2004. Effect of phytic acid and phytase on feed intake, growth, digestibility and trypsin activity in Atlantic salmon (Salmo salar, L.). Aquaculture Nutrition, 10(2), 135–142. http://doi.org/10.1111/j.1365-2095.2003.00290.x

Saleem, A., & M. K. H. Ebrahim. 2014. Production of amylase by fungi isolated from legume seeds collected in Almadinah Almunawwarah, Saudi Arabia. Journal of Taibah University for Science, 8(2), 90–97. http://doi.org/10.1016/j.jtusci.2013.09.002

Schuster, E., N. Dunn-Coleman, J. Frisvad, & P. Van Dijck. 2002. On the safety of Aspergillus niger - A review. Applied Microbiology and Biotechnology. http://doi.org/10.1007/s00253-002-1032-6

Selle, P. H., & V. Ravindran. 2007. Microbial phytase in poultry nutrition. Animal Feed Science and Technology. http://doi.org/10.1016/j.anifeedsci.2006.06.010

Shi, M., Y. Yang, Y. Li, Y. Wang, , & Z. Zhang. 2011. Optimum Condition of Ecologic Feed Fermentation by Pleurotus ostreatus Using Soybean Curd Residue as Raw Materials. International Journal of Biology, 3(4), 2. http://doi.org/10.5539/ijb.v3n4p2

Siala, R., F. Frikha, S. Mhamdi, M. Nasri, & A. S. Kamoun. 2012. Optimization of acid protease production by Aspergillus niger I1 on shrimp peptone using statistical experimental design. The Scientific World Journal, 2012, 1–11. http://doi.org/10.1100/2012/564932

Sivaramakrishnan, S., D.Gangadharan, K. M. Nampoothiri, C. R. Soccol, & A. Pandey. 2007. Alpha amylase production by Aspergillus oryzae employing solid-state fermentation. Journal of Scientific and Industrial Research, 66(8), 621–626.

Soccol, C. R., I. Iloki, B. Marin, & M. Raimbault, 1994. Comparative production of alpha-amylase, glucoamylase and protein enrichment of raw and cooked cassava by Rhizopus strains in submerged and solid. Journal of Food Science and Technology, 31(4), 320–323. Retrieved from http://agris.fao.org/agris-search/search/display.do?f=2012/OV/OV201204679004679.xml;IN19950029562

DOI: https://doi.org/10.21059/buletinpeternak.v41i1.13337