MATERIAL CuO/BENTONIT SEBAGAI BAHAN ANTIBAKTERI ESCHERICHIA COLI



Rachma Yulia Fatmawati(1), Karna Wijaya(2), Iqmal Tahir(3*)

(1) Laboratory of Physical Chemistry, Department of Chemistry, Universitas Gadjah Mada
(2) Laboratory of Physical Chemistry, Department of Chemistry, Universitas Gadjah Mada
(3) Laboratory of Physical Chemistry, Department of Chemistry, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Sintesis, karakterisasi, dan uji aktivitas antibakteri material CuO/bentonit telah dilakukan. Preparasi diawali dengan perlakuan aktivasi terhadap bentonit dengan H2SO4 untuk menghasilkan H-bentonit dan kemudian didispersikan dalam CuSO4∙5H2O, KOH, dan etanol untuk menghasilkan CuO/bentonit. Sintesis CuO/bentonit ini dilakukan dengan metode hidrotermal. Produk dikarakterisasi dengan FTIR, XRD dan SEM-EDS. Aktivitas antibakteri produk diuji dengan metode Agar Diffusion Test. Hasil penelitian menunjukkan jika CuO/bentonit telah berhasil terbentuk yang dikonfirmasi oleh difraktogram XRD dengan keberadaan puncak pada 2θ = 35,10; 38,34; dan 40,07o. Citra SEM menunjukkan bahwa modifikasi bentonit menjadi CuO/bentonit menyebabkan perubahan pada permukaan bentonit. Data EDX menunjukkan munculnya kandungan Cu di dalam CuO/bentonit sebesar 19,50% (b/b). Hasil uji antibakteri menunjukkan bahwa bentonit, CuO/bentonit, dan CuO memiliki aktivitas antibakteri. CuO/bentonit memiliki diameter daya hambat paling luas dibandingkan dengan bentonit, H/bentonit, dan CuO/bentonit

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