EFFECT OF 50% ETHANOLIC EXTRACT OF PEGAGAN HERB (Centella asiatica (L.) Urban) ON CELL PROLIFERATION OF LYMPHOCYTES IN Balb/c MALE MICE INDUCED BY HEPATITIS B VACCINE

Abstract

Pegagan (Centella asiatica (L.) Urban) has been known as multi effect plant that empirically used in the traditional medicine. This research aims to evaluate the effect of 50%ethanolic extract of pegagan herb against increased lymphocyte proliferation cells in Balb/c mice that induced with hepatitis B vaccine, and also to evaluate triterpen glicosida compound (asiaticosida) of 50% ethanolic extract of pegagan herb. The test was done by 60 Balb/c male mices that divided into 6 groups : control group, testing group that was treated with pegagan extract dose of 25 mg/KgBB, 50 mg/KgBB, 100 mg/KgBB, 200 mg/KgBB and 400 mg/KgBB. All of mices were vaccinated on day 0 and days 15. Furthermore, identification of triterpen glicosida compound was done by KLT method using BAW 4:1:5 as upper eluen. Lymphocyte cells were isolated and the cells proliferation measured by MTT reduction method. Data was analyzed statistically using 95% confidence level. The result shown that 50% of ethanolic extract of pegagan herb did not give  the immuomodulatory effect while the identification test shown that 50% of extract of pegagan herb has triterpen glicosida compound (asiaticoside).