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* Corresponding Author
Antioxidant Capacity from Ethyl Acetate Fraction of Kenikir (Cosmos Caudatus K)
https://doi.org/10.22146/mot.84604 Khrisna Pangeran(1), Tatang Irianti Irianti(2*), Hari Purnomo(3)
(1) Magister of Pharmaceutical Science, Faculty of Phramacy, Universitas Gadjah Mada
(2) Faculty of Phramacy, Gadjah Mada University
(3) Faculty of Phramacy, Gadjah Mada University
(*) Corresponding Author
Abstract
Antioxidant is an activity that inhibits oxidation reactions due to exposure to free radicals, the search for antioxidant compounds continues to be developed from natural sources. The purpose is to identify new sources of safe and inexpensive agents through antioxidant capacity from ethyl acetate fraction on kenikir leaves was evaluated through enzymatic and non-enzymatic in vitro models. The research began with the method of separating compounds from ethanol extracts and ethyl acetate fractions, quantification of total phenolics and flavonoids, antioxidant capacity tests with DPPH, and tyrosinase enzyme. The results of the study produced isolate E which was observed in a single stain with Rf 0,80. The compound has a typical marker worth 1734.07 cm-1 with an indication of stretching vibration movement on C-O. It has a maximum wavelength of ± 216 nm, with an abundance of 49.75% and a retention time of 27.20 minutes. TPC and TFC test results, the water fraction is the most dominant value with 0.430 mgGAE/g and 0.056 mQE/g. DPPH assay results, isolate E has an IC50 value of 162.03 ppm and a %inhibition of 12.73%. The tyrosinase inhibition test of L-DOPA substrate was resulting an IC50 value of 77.51 ppm compared to kojic acid of 16.48 ppm.
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Ahmad, A. R., Juwita, J., and Ratulangi, S. A. D. (2015). Penetapan kadar fenolik dan flavonoid total ekstrak metanol buah dan daun patikala (Etlingera elatior (Jack) RM SM). PSR, 2(1), 1.
Andarwulan, N., D. Kurniasih, R.A. Apriady, H. Rahmat, A.V. Roto and B.W. Bolling, (2012). Polyphenols, carotenoids and ascorbic acid in underutilized medicinal vegetables. J. Funct. Foods , 4(1): 339-347.
Chan EWC, Wong SK, and Chan HT. (2016) Ulam herbs of oenanthe javanica and cosmos caudatus: an overview on their medicinal properties. J. Nat Remedies.16(4):137-47
Cheng, S. H., Khoo, H. E., Ismail, A., Abdul-Hamid, A., and Barakatun-Nisak, M. Y. (2016). Influence of extraction solvents on Cosmos caudatus leaf antioxidant properties. Iran. J. Sci. Tecnhnol A, 40(1), 51–58. https://doi.org/10.1007/s40995-016-0007-x.
Cimpoiu, C., and Hodisan, T. (1997). Mobile Phase Optimization in Thin Layer Chromatography (TLC). Rev Anal Chem, 16(4), 299-322
Clayden, J., Greeves, N., and Warren, S. (2012). Organic chemistry. Oxford university press. 148.
Ding, C., Fan, X., and Wu, G. (2017). Peroxiredoxin 1–an antioxidant enzyme in cancer. JCMM, 21(1), 193-202.
Du, J. B., Tang, Y. L., Long, Z. W., Hu, S. H., & Li, T. (2014). Theoretical calculation of spectra of dibutyl phthalate and dioctyl phthalate. Russ. J. Phys. Chem, 88, 819-822.
El-Shouny, W. A., Gaafar, R. M., Ismail, G. A., & Elzanaty, M. M. (2017). Antibacterial activity of some seaweed extracts against multidrug resistant urinary tract bacteria and analysis of their virulence genes. Int. J. Curr. Microbiol. App. Sci, 6(11), 2569-2586.
Fessenden, R. J., and Fessenden, J. S. (1986). Kimia Organik edisi ketiga. Erlangga, Jakarta, 136.
Filippi, C., Buda, F., Guidoni, L., and Sinicropi, A. (2012). Bathochromic shift in green fluorescent protein: a puzzle for QM/MM approaches. J. Chem. Theory. Comput., 8(1), 112-124
Firdaus, M. D., Artanti, N., Hanafi, M., and Hanafi, M. (2021). Phytochemical Constituents , and In vitro Antidiabetic and Antioxidant Properties of Various Extracts of Kenikir ( Cosmos caudatus ) Leaves. Pharmacognosy Journal, 13(4), 890–895
Gandjar, I. G., & Rohman, A. (2007). Kimia farmasi analisis. Yogyakarta: Pustaka Pelajar, 224, 228.
Herlina, N., Riyanto, S., Martono, S., and Rohman, A. (2018). Antioxidant activities, phenolic and flavonoid contents of methanolic extract of Stelechocarpus burahol fruit and its fractions. Dhaka Univ. J. Pharm. Sci, 17(2), 153-159.
Hikmawanti, N. P. E., Fatmawati, S., Arifin, Z., Cahyaningrum, N., and Arif Fauzan, M. (2021). The Effect of Pre-Extraction Preparation on Antioxidant Compounds of Sauropus androgynus (L.) Merr. Leaves Extracts. PSR, 8(3), 2.
Irianti T, Purnomo H, and Kuswandi. (2019). Uji Penangkapan Radikal 2,2-Difenil-1-pikrilhidrazil oleh Ekstrak Etanol Bunga Kecombrang (Nicolaia speciosa (BI.) Horan) dan Buah Talok (Muntingia calabura L.). JTOI; 12 (1): 41-53
Irianti T, Puspitasari A. (2015). The Activity of Radical Scavenging of 2,2-Diphenyl-1-pycrilhydrazil (DPPH) by Ethanolic Extracts of Mengkudu Leaves (Morinda citrifolia L.), Brotowali Stem (Tinospora crispa L.), Its Water Fraction and Its Hydrolized Fraction. Trad. Med. J; 20 (3): 140-148.
Jia, L., Zhang, L., Ye, Y. hua, Li, J. lin, Cong, M., and Yuan, T. (2019). Effect and Mechanism of Elaeagnus angustifolia Flower and Its Major Flavonoid Tiliroside on Inhibiting Non-enzymatic Glycosylation. J. Agric. Food Chem, 67(50), 13960–13968. https://doi.org/10.1021/acs.jafc.9b06712.
Kang, N. J., Shin, S. H., Lee, H. J., and Lee, K. W. (2011). Polyphenols as small molecular inhibitors of signaling cascades in carcinogenesis. Pharmacol. Ther, 130, 310–324
Ko RK, Kim GO, Hyun CG, Jung DS, and Lee NH. (2011). Compounds with tyrosinase inhibition, elastase inhibition and DPPH radical scavenging activities from the branches of Distylium racemosum Sieb. Et Zucc. Phyto Ther Res. 1451–1456
Ladwani AMA, Salman M and Abdel Hameed ES. (2018). Chemical composition of Ocimum basilicum L. essential oil from different regions in the Kingdom of Saudi Arabia by using Gas chromatography mass spectrometer. J. Med. Plants Stud, 6(1), 14-19
Moshawih S, Manraj SC, Zuraini A, and Zainul A. (2017). A comprehensive review on Cosmos caudatus (Ulam Raja): pharmacology, ethnopharmacology, and phytochemistry. Int Res J Educ Sci; 1(1):14-31.
Murugesu, S., Ibrahim, Z., Ahmed, Q. U., Uzir, B. F., and Khatib, A. (2019). Identification of α-glucosidase inhibitors from Clinacanthus nutans leaf extract using liquid chromatography-mass spectrometry-based metabolomics and protein-ligand interaction with molecular docking. J. Pharm. Anal, 9(2), 91-99.
Oktaviani, E. (2018). Penentuan Kadar Flavanoid Dan Aktivitas Antioksidan Formula Serbuk Minuman Instan Ekstrak Belimbing Manis (Averrhoa Carambola L.). FJIF, 8(1), 1-12.
Priani, S. E., and Fakih, T. M. (2021). Insights into molecular interaction of flavonoid compounds in citrus peel bound to collagenase and elastase enzymes: A computational study. PSR, 8(2), 5.
Shah NA, Khan MR, Naz K, and Khan MA (2014) Antioxidant potential, DNA protection, and HPLC-DAD analysis of neglected medicinal Jurinea dolomiaea roots. Biomed Res Int 2014:726241
Shanab, S. M., Shalaby, E. A., Lightfoot, D. A., & El-Shemy, H. A. (2010). Allelopathic effects of water hyacinth [Eichhornia crassipes]. PloS one, 5(10), e13200.
Shi G-J, Li Y, Cao Q-H, Wu H-X, Tang X-Y, Gao X-H, et al. (2019). In vitro and In vivo evidence that quercetin protects against diabetes and its complications: A systematic review of the literature. Biomed Pharmacother;109:1085-99
Shui, G., Leong, L. P., and Wong, S. P. (2005). Rapid screening and characterisation of antioxidants of Cosmos caudatus using liquid chromatography coupled with mass spectrometry. J. Chromatogr, 827(1), 127-138
Sopiah B, Muliasari H, and Yuanita E. (2019). Skrining Fitokimia dan Potensi Aktivitas Antioksidan Ekstrak Etanol Daun Hijau dan Daun Merah Kastuba. J. Ilm. Kefarm; 17 (1): 27-33.
Syarifah, A., Budiman, A., and Nazilah, S. A. (2021). Formulation and Antioxidant Activity of Serum Gel of Ethyl Acetate Fraction From Musa x paradisiaca L. In 4th ICoSIHSN.. (pp. 310-315). Atlantis Press
DOI: https://doi.org/10.22146/mot.84604
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Faculty of Pharmacy
Universitas Gadjah Mada