IDENTIFICATION OF ANTIBACTERIAL COMPOUNDS ISOLATED FROM Leucaena leucocephala (Lam.) De Wit.) LEAVES

https://doi.org/10.22146/tradmedj.8138

Ari Sartinah(1*), Puji Astuti(2), Subagus Wahyuono(3)

(1) Faculty of Pharmacy, University of Hassanuddin
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Plants are important natural resources widely explored for maintaining health and treatment of diseases. One of them is Leucaena leucocephala leaf which is used as traditional medicine. Indonesian has used this leaf to treat wound and inflammation. This study was conducted to isolate and identify antibacterial compounds from L. leucocephala leaves. Dry powder of this leaf was extracted using washbenzen by Soxhletation method followed by methanol extraction. Both extracts were tested for antibacterial activity against Staphylococcus aureus ATCC 25923 dan E. coli 25922 and analysed by TLC. Positive extract to S. aureus was further separated by vacuum liquid chromatography using different combination of washbenzene and ethyl acetate followed by analysis using TLC. Each fraction was tested for antibacterial activity and analysed by TLC. The positive fraction was further separated by TLC-preparative, assigned as p1a, p2a, p3a. The active compound (p2a) was tested for its purity using TLC with various mobile phase. Its structure was identified using combination data of UV-Vis and IR spectrophotometry, GC-MS and NMR.  Based on UV-Vis data, this compound showed the absence of chromophoric group.  IR data showed adsorption at 3409,4 cm-1 (OH), 2928,2 cm-1(CHaliphatic), 2854,3 cm-1(CHaliphatic), 1575,5 cm-1 (C=C), 1416,4 cm-1 (CH2), 1385,0 cm-1(CH3), 1258,3 cm-1 and 1082,3 cm-1 (C-O). GC-MS data showed molecule ion at m/z 482   (M + H+)and fragmented ion at  m/z 427   (M + H+). 1H-NMR (CDCl3) spectrum showed resonance at δ 0,8; 1,4; 1,6; 2,0; 2,3; 3,6; 4,2 and  5,4 pp, suggesting that this compound is lupeol. 


Keywords


L. leucocephala (Lam.) de Wit., antibacterial compounds, isolation



References

Anonim, 2008, Indonesia Country Profile 2007, Kementrian Kesehatan RI, Jakarta

Bussman, R. W., Glenn, A., Sharo, D., 2010, Antibacterial Activity of Medical Plants of Northen Peru - Can Traditional Applications Provide Leads for modern Science?, Indian J. of Traditional Knowledge, 9(4): 742-743

Chanwitheesuk, A., Teerawutgulrag, A., Rakariyatham, N., 2005, Screening of Antioxidant Activity and Antioxidant Compounds of Some dible Plants of Thailand, J. Food Chemistry, 92 : 491–497

Direkbusarakom, S., 2004, Application of Medicinal Herbs to Aquaculture in Asia, Walailak J Science & Tech, 1(1): 7-14

Igoli, John, O. dan Alexander, G.I., 2008, Friedelanone and Other Triterpenoid from Hymenocardia acida, International journal of Physical Science, 9(6): 156-158

Jawetz, E., Melnick, J. L. dan Adelberg, E.A., 1982, Mikrobiologi untuk Profesi Kesehatan, edisi 14, diterjemahkan oleh Bonang, EGC, Penerbit Buku Kedokteran, Jakarta

Mathers, C. D. dan Loncar, D., 2005, Updated Projections of Global Mortality and Burden of Disease, 2002-2030:Data Sources, Methods and Results, Evidence and Information for Policy World Health Organization

Nhereraa, F.V., Ndlovua L.R. dan Dzowelab, B.H., 1998, Utilisation of Leucaena diversifolia, Leucaena esculenta, Leucaena pallida and Calliandra calothyrsus as nitrogen supplements for growing goats fed maize stover, Animal Feed Science and Technology, 74: 15-28

Pratiwi, S. T., 2008, Mikrobiologi Farmasi, Penerbit Erlangga, Jakarta

Radji, M., 2005, Peranan Bioteknologi dan Mikroba Endofit dalam Pengembangan Obat Herbal, Majalah Ilmu Kefarmasian, 3 : 113-126.

Robinson, T., 1995, Kandungan Organik Tumbuhan Tingkat Tinggi, ITB Press, Bandung

Saiful, 2005, Isolasi dan Identifikasi Senyawa Antimikroba dari Daun Galinggang (Cassia alata Linn). Tesis, Universitas Gadjah Mada, Yogyakarta

Saucedo, G., Alvarez, Jimenez dan Arriaga., 1980, Leucaena leucocephala as a Supplement for Milk Production on Tropical Pastures with Dual Purpose Cattle, Trop Animal Product, 5: 1

Silverstein, R.M., Bassler, G.C. dan Morrill, T.C., 2002, Spectrometric Identification of Organic Compounds, Seventh Edition., Jhon Wiley & Sons Inc., USA

Skerman, P.J., 1977, Tropical Forage Legumes, FAO: Plant Production and Protection Series No. 2

Wahyuono, S., 1985, Phytochemical Investigation of Amsonia grandiflora Family Apocynaceae, Thesis, The University of Arizona

Wahyuni, 2006, Efek Antiinflamasi Infusa Daun Petai Cina pada Tikus Jantan Galur Wistar, Skripsi, Fakutas Farmasi UMS, Surakarta



DOI: https://doi.org/10.22146/tradmedj.8138

Article Metrics

Abstract views : 2140 | views : 24468

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2010 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada