FLAVONOID PROFILE OF Clitoria ternatea Linn

https://doi.org/10.22146/tradmedj.8083

Selvamaleeswaran Ponnusamy(1*), Wesely Ebenezer Gnanaraj(2), Johnson Marimuthu Antonisamy(3)

(1) Research and Development centre, Bharathiar University, Coimbatore,Tamil Nadu, India
(2) Department of Botany, Arignar Anna Government Arts College, Namakkal,Tamil Nadu, India
(3) Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, India
(*) Corresponding Author

Abstract


The present study was aimed to reveal the flavonoid profile of Clitoria ternatea L leaves, stem and seeds using the HPTLC analysis. The powdered leaves, stem and seed samples were extracted with 150 mL of methanol for 8-12 h by using the Soxhlet apparatus. The Ethyl acetate-butanone-formic acid-water (5:3:1:1) was employed as mobile phase for flavonoids. The developed plate was sprayed with 1% ethanolic aluminium chloride as spray reagent and dried at 100°C in hot air oven for 2min. The plate was photo-documented at UV 366 nm and daylight using Photo-documentation chamber.The methanolic extract of stem, leaves and seeds of Clitoria ternatea showed the presence of 24 bands with 18 different Rf values with range 0.01 to 0.96.  Out of 24 bands, 10 bands with seven Rf values viz., 0.04, 0.10, 0.28, 0.37, 0.49, 0.65 and 0.85 were idenified as flavonoids, the other bands were noted as unknown metabolites. Developed HPTLC chromatogram of Clitoria ternatea methanolic extracts of vegetative and reproductive parts could be used efficiently for identification, and quality assessment of the plant in the pharmaceutical industries. These profiles may used as chemical marker to solve plant systematic problems.

Keywords


Clitoria ternatea; HPTLC; Chromatography; Flavonoids

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References

Appia Krishnan, G., Rai, V.K., Nandy, B.C., Meena, K.C., Dey, S., Tyagi, P.K., Tyagi, L.K. 2009. Hypoglycemic and Antihyperlipidaemic Effect of Ethanolic Extract of Aerial Parts of Aerva lanata Linn. in Normal and Alloxan induced Diabetic Rats . IJPSDR, 1(3): 191-194

Deshmukh, T., Yadav, B.V., Badole, S.L., Bodhankar, S.L., Dhaneshwar, S.R. (2008). Antihyperglycaemic activity of alcoholic extract of Aerva lanata (L.) A. L. Juss. Ex J. A. Schultes leaves in alloxan induced diabetic mice. J. Appl. Biomed. 6: 81–87

Harborne JB. 1998. Phytochemcial methods (3rd Edn.). Chapman and Hall: London

Hari prasad, P., Ramakrishnan, N. 2012. Chromatographic finger print analysis of Rumex vesicarius L. by HPTLC Technique. Asian Pacific Journal of Tropical Biomedicine 1-2

Karthikeyan, S., Sivakumar, A., Anbalagan, M., Nalini, E., Gothandam, K. M. 2013. Finger Printing of Alkaloids, Steroids and Flavonoids using HPTLC of Leucas aspera L. Whole Plant Methanolic Extract. J. Pharm. Sci. & Res. 5(3); 67 – 71

Kumar, V., Mukherjee, K., Kumar, S., Mal, M., Mukherjee, P.K. 2008. Validation of HPTLC method for the analysis of taraxerol in Clitoria ternatea. Phytochem Anal. 19(3):244-50

Manokaran, S., Jaswanth, A., Sengottuvelu, S., Nandhakumar, J., Duraisamy, R., Karthikeyan, D., Mallegaswari, R. 2008. Hepatoprotective Activity of Aerva lanata Linn. Against Paracetamol Induced Hepatotoxicity in Rats. Research J. Pharm. and Tech. 1(4): 398-400

Mhaskar, A.V., Prakash, K., Vishwakarma, K.S., Maheshwari, V.L. 2010. Callus induction and antimicrobial activity of seed and callus extracts of Clitoria ternatea.L .Current trends in biotechnology and pharmacy 3(4): 561-567

Parimaladevi, B., Boominathan, R., Mandal, S.C. 2003. Anti-inflammatory, analgesic and antipyretic activities of Clitoria Ternatea root. Fitoterapia 74: 345-347

Rajkumar, T., Sinha, B.N. 2010. Chromatographic finger print analysis of budmunchiamines in Albizia amara by HPTLC technique. Int. J. Res. Pharm. Sci. 1(3): 313-316

Ram Mauji, Abdin, M.Z., Khan, M.A., Jha Prabhakar. 2011. HPTLC fingerprint analysis: A Quality control of Authentication of Herbal Phytochemicals. Springer Verlag: Berlin Heidelberg; 105

Shirwaikar, A., Issac, D., Malini, S. 2004. Effect of Aerva lanata on cisplatin and gentamicin models of acute renal failure. J. Ethnopharmacol. 90: 81–86

Toma´s-Barbera´n, F.A., Isabel Martos, Federico Ferreres, Branka S Radovic, Elke Anklam. 2001. HPLC flavonoid profiles as markers for the botanical origin of European unifloral honeys. J Sci Food Agric 81; 485 - 496

URL, 2010. http://lpi.oregonstate.edu/f-w00/flavonoid.html

Wagner, H., Baldt, S., Zgainski, E.M. 1996. Plant Drug Analaysis. Springer: Berlin

Wasim Aktar, M.D., Rajlakshmi Poi, Anjan Bhattacharya. 2008. Status of sennosides content in various Indian herbal formulations method standardization by HPTLC. Bangladesh J Pharmacol 3: 64-68

WHO, 1998. Quality Control Method for Medicinal Plant Material. Geneva. 1-15. Yamunadevi, M., Wesely, E. G., Johnson, M. 2012. Chromatographic fingerprint analysis on flavonoids constituents of the medicinally important plant Aerva lanata L. by HPTLC technique. Asian Pacific Journal of Tropical Biomedicine S8-S12



DOI: https://doi.org/10.22146/tradmedj.8083

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