FLAVONOID PROFILE OF Clitoria ternatea Linn

https://doi.org/10.22146/tradmedj.8083

Selvamaleeswaran Ponnusamy(1*), Wesely Ebenezer Gnanaraj(2), Johnson Marimuthu Antonisamy(3)

(1) Research and Development centre, Bharathiar University, Coimbatore,Tamil Nadu, India
(2) Department of Botany, Arignar Anna Government Arts College, Namakkal,Tamil Nadu, India
(3) Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, India
(*) Corresponding Author

Abstract


The present study was aimed to reveal the flavonoid profile of Clitoria ternatea L leaves, stem and seeds using the HPTLC analysis. The powdered leaves, stem and seed samples were extracted with 150 mL of methanol for 8-12 h by using the Soxhlet apparatus. The Ethyl acetate-butanone-formic acid-water (5:3:1:1) was employed as mobile phase for flavonoids. The developed plate was sprayed with 1% ethanolic aluminium chloride as spray reagent and dried at 100°C in hot air oven for 2min. The plate was photo-documented at UV 366 nm and daylight using Photo-documentation chamber.The methanolic extract of stem, leaves and seeds of Clitoria ternatea showed the presence of 24 bands with 18 different Rf values with range 0.01 to 0.96.  Out of 24 bands, 10 bands with seven Rf values viz., 0.04, 0.10, 0.28, 0.37, 0.49, 0.65 and 0.85 were idenified as flavonoids, the other bands were noted as unknown metabolites. Developed HPTLC chromatogram of Clitoria ternatea methanolic extracts of vegetative and reproductive parts could be used efficiently for identification, and quality assessment of the plant in the pharmaceutical industries. These profiles may used as chemical marker to solve plant systematic problems.

Keywords


Clitoria ternatea; HPTLC; Chromatography; Flavonoids

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DOI: https://doi.org/10.22146/tradmedj.8083

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