Bioautography and FTIR Analysis of Ethanol Fraction Morel Berry Root (Physalis angulata L.) Against Staphylococcus epidermidis and Pseudomonas aeruginosa

https://doi.org/10.22146/mot.78171

Pratika Viogenta(1*), Latifah Megasari(2), Laila Susanti(3)

(1) Department of Pharmacy, University of Lambung Mangkurat, Banjarbaru, South Kalimantan
(2) Department of Pharmacy, University of Tulang Bawang, Bandar Lampung, Lampung
(3) Department of Pharmacy, University of Tulang Bawang, Bandar Lampung, Lampung
(*) Corresponding Author

Abstract


Morel berry root contains flavonoids, tannins, and alkaloids that have an antibacterial characteristics. This study aims to determine the antibacterial activity of the morel berry root ethanol fraction against Staphylococcus epidermidis and Pseudomonas aeruginosa and the most effective active compounds to inhibit such activity. Extractions of morel berry root used the maceration method with ethanol 70% of and then continued by fractionation using ethanol, chloroform, and n-hexane. Test of the compound fraction was performed using Thin Layer Chromatography (TLC) and characterization with FTIR Spectrophotometer. The results of the antibacterial activity with inhibitory zone diameter were at a 100% concentration of 18.69 mm in S. epidermidis while P. aeruginosa of 20.00 mm. Bioautographic results for ethanol fraction indicate an inhibited zone with an Rf value of 0.72 in the S. epidermidis and P. aeruginosa which is thought to be a flavonoid compound. Characterization with FTIR spectrophotometer functional groups O-H phenol, C = C aromatic, C-H aromatic, C-O alcohol, and C-H aliphatic in ethanol fraction with Rf 0.72.

Keywords


Ciplukan; Thin Layer Chromatography; Antibacteria

Full Text:

PDF


References

Akbar, N.M.K., Ernah, E., 2018, ’Strategi pengembangan agribisnis buah ciplukan (Physalis peruviana) Di Waaida Farm, Jawa Barat’, Agricore J Agribisnis dan Sos Ekon Pertan Unpad. 3(2),37–47.

Andr, S., Jelinski, M., Zaheer, R., 2022,’ Bovine respiratory disease: conventional to culture-independent approaches to studying antimicrobial resistance in North America’, MDPI Antibiotics, 11(487),1–26.

Anggreany, R.T., Rahmawati, I., Leviana, F., 2020 ‘Uji antibakteri ekstrak dan fraksi herba ceplukan (Physalis angulata L.) untuk mengatasi infeksi Staphylococcus epidermidis selama persalinan’, Din Kesehat J Kebidanan dan Keperawatan, 11(1), 253–63.

Davis, W.W., Stout, T.R., 1971, ‘Disc plate method of microbiological antibiotic assay. II. novel procedure offering improved accuracy’, Appl Microbiol, 22(4), 666–670.

Ekawati, M.A., Suirta, I.W., Santi, S.R., 2017, ‘Isolasi dan identifikasi senyawa flavonoid pada daun sembukan (Paederia foetida L) serta uji aktivitasnya sebagai antioksidan’, J Kim, 11(1), 43–48.

Ergina, S.N., Pursitasari, I.D., 2014, ‘Uji kualitatif senyawa metabolit sekunder pada daun palado (Agave angustifolia) yang diekstraksi dengan pelarut air dan etanol’, J Akad Kim, 3(3),165–72.

Isnaeni, I., Astuti, A., Yuwono, M., 2018, ‘Validation of thin-layer chromatography-bioautographic method for determination of streptomycin’, J Farm dan Ilmu Kefarmasian Indones,4(1), 32–38.

Jesionek, W., Grzelak, E.M., Majer-Dziedzi. B., 2013, ‘Chom IM. Thin-layer chromatography - Direct bioautography for the screening of antimicrobial properties of plant extracts’, J Planar Chromatogr - Mod TLC, 26(2),109–113.

Kaya, B., Menemen, Y., Saltan, F.Z., 2012, ‘Flavonoid compounds identified in Alchemilla L. species collected in the north-eastern black sea region of Turkey’, African J Tradit Complement Altern Med, 9(3), 418–425.

Luthfiyanti, R., Iwansyah, A.C., Rahayu, Y., Achyadi, N.S., 2021, ‘Study of antioxidant activities, acceptability, and shelflife prediction of Morel berry (Physalis angulata L.) juice drinks’, In: IOP Conference Series: Materials Science and Engineering. p. 1–11.

Parbuntari, H., Prestica, Y., Gunawan, R., Nurman, M.N., Adella, F., 2018, ‘Preliminary phytochemical screening (qualitative analysis) of cacao leaves (Theobroma cacao L.)’, EKSAKTA Berk Ilm Bid MIPA, 19(2),40–45.

Souto, J., Rocha, W., Nunes, L., Chaves, M., Alves, H., Cato, R., 2021, ‘Antimicrobial screening of extracts and fractions from Piper species using bioautografy method’, Res Soc Dev, 10(6), 4–11.

Utami, S., Widiyantoro, A., Jayuska, A., 2016, ‘Karakterisasi senyawa fenolik dari fraksi metanol bunga nusa indah (Mussaenda erythrophylla). J Kim Khatulistiwa 6(4), 83–88. Available from: https://jurnal.untan.ac.id/index.php/jkkmipa/article/view /22520

Valiyeva, A., Garaev, E.A., 2019, 'Thin Layer chromatography of alkaloids from hyoscyamus niger thin layer chromatography of alkaloids from hyoscyamus niger leaves', In: International Symposium on the Chemistry of Natural Compounds. p. 97.

Viogenta, P., Wahidah, L.K., Saputri, I.H., 2017, 'Aktivitas antibakteri ekstrak akar ceplukan (Physalis angulata L.) terhadap Staphylococcus epidermidis dan Pseudomonas aeruginosa, J Farm Lampung, 6(2), 38–45.



DOI: https://doi.org/10.22146/mot.78171

Article Metrics

Abstract views : 1352 | views : 1221

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2022 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada