Ethanolic Extract of Pluchea indica Less leaf Increases Serum Growth Hormone in Lactating Rats

https://doi.org/10.22146/mot.62060

Rul Afiyah Syarif(1*), Nungki Anggorowati(2), Mia Munawaroh(3), Mae Sri Hartati Wahyuningsih(4)

(1) 1) Department of Pharmacology and Therapy, Faculty of Medicine Public Health and Nursing, Universitas Gadjah Mada 2) Herbal Medical Center, Faculty of Medicine Public Health and Nursing, Universitas Gadjah Mada
(2) Department of Anatomical Pathology, Faculty of Medicine Public Health and Nursing, Universitas Gadjah Mada
(3) Department of Pharmacology and Therapy, Faculty of Medicine Public Health and Nursing, Universitas Gadjah Mada
(4) 1) Department of Pharmacology and Therapy, Faculty of Medicine Public Health and Nursing, Universitas Gadjah Mada 2) Herbal Medical Center, Faculty of Medicine Public Health and Nursing, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The objectives of the study were to evaluate the effect of ethanolic extract of P. indica Less leaf (EPI) on serum growth hormone (GH), milk yield, body weight gain of dams, and dam’s organ weight in lactating rats. A total of 25 lactating rats with six pups were randomized and distributed to one of five treatments (control (RO water), standard (domperidone 2,5 mg/kg BW/day), EPI 250 (250 mg/kg BW of EPI), EPI 500 (500 mg/kg BW of EPI), and EPI 750 (750 mg/kg BW of EPI)). The treatment was administered daily starting from the 2nd until the 15th day of lactation. On the 16th day serum growth hormone level, body and organ weight of rats were measured. Serum GH levels in the EPI 750 group (1963.25 ± 360.91 pg/µL) increased significantly compared to the domperidone (409.46 ± 28.80 pg/µL) and the control group (723.40 ± 95.78 pg/µL, p0.05). There was significant body weight gain in the EPI 750 group compared with the domperidone group. There was no significant difference in organ weight gain in each group. The study revealed that ethanolic extract of P. indica Less leaf can increase serum growth hormone in lactating rats.

Keywords


Pluchea indica; growth hormone; milk production; body weight

Full Text:

PDF


References

Amalina, N., Suyatmi, Suparyanti, E.L. 2010. Effect of beluntas (Pluchea indica) leaf extract on mice spermatogenesis. Journal of Natural Product Biochemistry 8(2): 47-51

Alamer, M.A., Basiouni, G.F. 2005. Feeding effects of fenugreek seeds (trigonella foenum-graecum l.) on lactation performance, some plasma constituents and growth hormone level in goats. Pak J Biol Sci 8(11):1553-6.

Bao, Z., Lin, J., Ye, L., Zhang, Q., Chen, J., Yang, Q., et al. 2016. Modulation of mammary gland development and milk production by growth hormone expression in GH transgenic goats. Front Physiol 7:278.

Buapool, D.,Mongkol, N.,Chantimal, J.,Roytrakul, S.,Srisook., E,Srisook, K. 2013. Molecular mechanism of anti-inflammatory activity of Pluchea indica leaves in macrophages RAW 264.7 and its action in animal models of inflammation. J Ethnopharmacol146(2):495-504

Desmawati, D and Sulastri, D. 2019. Phytoestrogens and their health effect. Journal of Medical Sciences 7(3): 495-499.

Grzeskowiak, L.E., Smithers, L.G., Amir, L.H., Grivell, R.M. 2018. Domperidon for increasing breastmilk volume in mothers expressing breast milk for their preterm infants: a systematic review and meta-analysis. BJOG 125(11):1371-8.

Hale, T.W, Kendall-Tackett, K., and Cong, Z. 2018. Domperidone versus metoclopramide: self-reported side effects in a large sample of breastfeeding mothers who used these medications to increase milk production. Clinical Lactation 9:10–7

Hull, K.L., Harvey, S. 2014. Growth hormone and reproduction: a review of endocrine and autocrine/paracrine interactions. Int J Endocrinol 234014. doi: 10.1155/2014/234014

Javed, A and Lteif, A. 2013 Development of the Human Breast. Semin Plast Surg 27:5–12.

Jeng, Y.J., Kochukov, M., Nauduri, D., Kaphalia, B.S., and Watson, C.S. 2010. Subchronic exposure to phytoestrogens alone and in combination with diethylstilbestrol – pituitary tumor induction in Fischer 344 rats. Nutrition & Metabolism 7:40. http://www.nutritionandmetabolism.com/content/7/1/40

Kao, C.L,Cho, J.,Lee, Y.Z.,Cheng, Y.B.,Chien, C.Y.,Hwang, C.F.,Hong, Y.R,,Tseng, C.N.,Cho, C.L. 2015. ethanolic extracts of Pluchea indica induce apoptosis and antiproliferation effects in human nasopharyngeal carcinoma cells. Molecules20(6):11508-11523

Lompo-Ouedraogo, Z., Heide, D., Beek, E.M., Swarts, H.J.M., Mattheij, J.A.M., and Sawadogo, L. 2004. Effect of aqueous extract of Acacia nilotica ssp adansonii on milk production and prolactin release in the rat. J Endocrinol 182: 257–266

Madon, R.J., Ensor, D.M., Knight, C.H., Flint, D., 1986. Effects of an antiserum to rat growth hormone on lactation in the rat. J Endocrinol 111(1):117-23.

Ni, Y., Chen, Q., Cai, J., Xiao, Zhang, J. 2020.Three lactation-related hormones: regulation of hypothalamus-pituitary axis and function on lactation. Mol Cell Endocrinol 111084. https://doi.org/10.1016/j.mce.2020.111084.

Nahdi, M.S., Martiwi, I.N.A., dan Arsyah, D.C. 2016. The ethnobotany of medicinal plants in supporting the family health in Turgo, Yogyakarta, Indonesia. Biodiversitas 17(2): 900906

Pargaputri, A.F., Munadziroh, E., and Indrawati, R. 2016. Antibacterial effects of Pluchea indica Less leaf extract on E. faecalis and Fusobacterium nucleatum (in vitro). Dental Journal 49(2): 93–98

Pramanik, K.C., Bhattacharya, P., Biswas, R., Bandyopadhyay, D., Mishra, M., and Chatterjee, T.K., 2006. Hypoglycemic and antihyperglycemic activity of leaf extract of Pluchea indica Less. Orient Pharm Exp Med 6(3): 232-236

Sa’roni, Sadjimin, T., Sja’bani, M., dan Zulaela. 2004. Effectiveness of the Sauropus androgynus (L.) Merr leaf extracts in increasing mother’s breast milk production. Media Litbang Kes 14:20-24

Sternlicht, M.D., Kouros-Mehr, H., Lu, P., and Werb, Z. 2006. Hormonal and local control of mammary branching morphogenesis. Differentiation 74: 365–381. doi:10.1111/j.1432-0436.2006.00105.x.

Susila, A.D, Syukur, Dharma, H.P.K., Gunawan, E., Evi. 2012. Koleksi dan Indentifikasi Tanaman Sayuran Indigenous. Pusat Kajian Hortikultura Tropika. Institut Pertanian Bogor. P: 1-3.

Sumanth, M dan Narasimharaju, K. 2011. Evaluation of galactagogue activity of lactovedic: a polyherbal formulation. International Journal of Green Pharmacy 5(1): 61-65.

Suriyaphan, O. 2014. Nutrition, health benefits and applications of Pluchea indica (L.) Less leaves. Mahidol University Journal of Pharmaceutical Sciences 41 (4): 1-10.

Tabares, F.P., Jaramillo, J.V.B., and Ruiz-Cortés, Z.T. 2014. Review article: pharmacological overview of Galactogogues. Vet Med Int, p: 1-20/

Valkenburg, J.L.C.H dan Bunyapraphatsara, N. 2001. Plant Resources of South-East Asia No 12(2). Medicinal and Poisonous Plants 2. Backhuys Publishers, Leiden. pp: 441-443.

Widyawati, P.S., Budianta, T.D.W., Gunawan, D.I., Ribka Stefanie Wongso, R.S. 2015. Evaluation antidiabetic activity of various leaf extracts of Pluchea indica Less. International Journal of Pharmacognosy and Phytochemical Research 7(3): 597-603.



DOI: https://doi.org/10.22146/mot.62060

Article Metrics

Abstract views : 1884 | views : 1775

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2021 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada