Sunscreen Activity of Fraction n-hexane, Chloroform, and Eethyl Acetate of Ethanol 96% Flamboyan Leaf (Delonix regia. Raf) Extract

https://doi.org/10.22146/mot.54425

Putra Jiwamurwa Pama Tjitda(1*), Febri Odel Nitbani(2), Maria Kristina Bangko(3)

(1) Department of Pharmacy, Health Polytechnic Kemenkes Kupang
(2) Department of Chemistry, Faculty of Science and Engineering, Nusa Cendana University
(3) Department of Pharmacy, Health Polytechnic Kemenkes Kupang
(*) Corresponding Author

Abstract


The sunscreen test of fraction n-hexane, chloroform, and ethyl acetate of ethanol 96% flamboyant leaf (Delonix regia. Raf) extract had been performed. This research begins the extraction of a flamboyant leaf using 96% ethanol. Extraction used the maceration method. The extract is fractionation with some solvents such as n-hexane, chloroform, and ethyl acetate. Each fraction was identified secondary metabolite and sunscreen test involving SPF, %Te, and %Tp measurement using UV-Vis Spectrophotometer. The result of phytochemical screening exhibited flavonoids, alkaloids, saponins, tannins, and phenolics in ethyl acetate fraction. n-hexane and chloroform fraction don't show saponins and flavonoid content. The sunscreen test shows that chloroform fraction has good protection power toward UV light with SPF, %Te, and %Tp value is 54.27±0.462, 7.46±0.473, and 12.83±0.047 in 250 mg/L, respectively.


Keywords


Flamboyant leaf (Delonix regia. Raf); Sunscreen; Maceration

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References

Bustamante, Y.A.M., Mejia, M.A.P., & Giraldo, J.C.M., 2020, ‘Comparison of the photoprotective effect between hydrolyzed and aglycones flavonoids as sunscreen: A systematic review’, J. app. pharm. sci. 10, 116–123.

Cowan, M.M., 1999, ‘Plant products as antimicrobial agents’. Clin. Microbiol. Rev. 12, 564–582.

Donglikar, M.M., & Deore, L.S., 2016, ‘Sunscreens: A review’. Phcogj. 8, 171–179.

Esan, I.R., Bankole, O., Shittu, A., Ogunfeitimi, O., & Sosany, P., 2020, ‘In Vitro antioxidant Activities of Aqeous Leaf Extract of Delonix Regia’. IJCIRAS. 2, 146–154.

Gafur, M.A., & Bialangi, N., 2013, ‘Isolasi dan Identifikasi Senyawa Flavonoid Dari Daun Jamblang (Syzygium Cumini)’, Skripsi, Program Studi Kimia, Fakultas MIPA, Universitas Negeri Gorontalo, Gorontalo.

Gizawy, H.A.E., Alazzouni, A.S., & Haddad, A.E.E., 2018, ‘Pharmacognostical and Biological Studies of Delonix regia growing in Egypt: HPLC Profiles’, Pharmacogn. Commn. 8, 125–131.

Manaia, E.B., Kaminski, R.C.K., Corrêa, M.A., & Chiavacci, L.A., 2013, ‘Inorganic UV filters’, Braz. J. Pharm. Sci. 49, 201–209.

Ngurah, B.I.G.M., Jumina, Anwar, C., Mustofa, M., & Sahadewa, S., 2014, ‘Synthesis of Benzoyl C-Phenylcalix[4]resorcinaryl Octaacetate and Cinnamoyl C-Phenylcalix[4]arene for UV Absorbers’, Indones. J. Chem. 14, 160–167.

Saewan, N., & Jimtaisong, A., 2013, ‘Photoprotection of natural flavonoids’, J. App Pharm Sci. 3, 129–141.

Siregar, C.S.D., Reveny, J., & Dalimunthe, A., 2019, ‘Formulation and Clinical Evaluation of Anti-Aging Activity of Blemish Balm Cream Vitamin E and Determination of SPF Value with Spectrophotometry’, Asian. J. Pharm. Res. Dev. 7, 35–42.

Suhane, N., Shrivastava, R.R., & Singh, M., 2016, ‘Gulmohar an ornamental plant with medicinal uses’, J. Pharmacogn. Phytochem. 5, 245–248.

Tjitda, P.J.P., 2018, 'Potensi Ekstrak Daun Flamboyan (Delonix regia) Asal Kupang sebagai Sunscreen', Prosiding Seminar Ilmiah Sains dan Teknologi, Fakultas Pertanian Universitas Timor, Kefamenanu, November 8, 2018, pp. 42–44.

Tjitda, P.J.P., & Nitbani, F.O., 2019. ‘Skrining Fitokimia Ekstrat Metanol, Kloroform, dan n-Heksan Daun Flamboyan (Delonix regia. Raf) Asal Kupang’, JSTK. 13, 70–79.

Warnida, H., & Nurhasnawati, H., 2017, ‘Efektivitas Ekstrak Daun Kokang (Lepisanthes amoena) Sebagai Tabir Surya; Eksplorasi Kearifan Lokal Kalimantan Timur’, Jur. Pen. Eko. Dip. 3, 57–62.

Wu, D., Yuan, P., Ke, C., Xiong, H., Chen, J., Guo, J., Lu, M., Ding, Y., Fan, X., Duan, Q., Shi, F., & Zhu, F., 2016, ‘Salidroside suppresses solar ultraviolet-induced skin inflammation by targeting cyclooxygenase-2’, Oncotarget 7, 25971–25982.





DOI: https://doi.org/10.22146/mot.54425

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