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* Corresponding Author
Behavioral Improvements and its Molecular Mechanism of Ilex kudingcha C.J. Tseng on Animal Model of Alzheimer’s Disease
https://doi.org/10.22146/mot.53686 Lap Thi Nguyen(1*), Nguyen Huu Son(2), Tran Nguyen Hong(3), Nguyen Minh Khoi(4), Kinzo Matsumoto(5), William R. Folk(6), Masamitsu Yamaguchi(7), Pham Thi Nguyet Hang(8)
(1) Hanoi University of Pharmacy, Hanoi
(2) 1) Hanoi University of Pharmacy, Hanoi 2) National Institute of Medcial Materials, Hanoi
(3) National Institute of Medicinal Materials, Hanoi
(4) National Institute of Medicinal Materials, Hanoi
(5) Institute of Natural Medicine, University of Toyama, Japan
(6) University of Missouri
(7) Kyoto Institute of Technology, Kyoto
(8) National Institute of Medicinal Materials, Hanoi
(*) Corresponding Author
Abstract
Alzheimer's disease (AD) is a common chronic neurodegenerative disease with well-defined pathophysiological mechanisms. Ilex kudingcha (IK) C.J. Tseng is commonly known as bitter tea or “Khom” tea in Vietnam. The present study was conducted to investigate the anti-dementia effect of IK using olfactory bulbectomized (OBX) mice. OBX mice were daily treated with IK extract (540 mg/kg) or reference drug, tacrine (2.5 mg/kg) 1 week before and continuously for 3 days after the OBX surgery. The object recognition test, modified Y maze test and fear conditioning test were employed to analyze non-spatial short-term, spatial short-term and long-term memories of the mice respectively. Administration of IK extract and tacrine attenuated these OBX-induced cognitive deficits in mice. The effects of IK and tacrine on spatial short-term memory impairment were reversed by scopolamine, a muscarinic receptor antagonist. The amyloid-beta (Aβ) production in adult transgenic Drosophila brain flies was also investigated by using Western blotting with APP-HA antibody. These results indicated that IK extract improves short-term and long-term memory disturbances in OBX mice and that muscarinic receptor may play a role on these actions. In addition, our result also showed that IK extract reduces the expression of amyloid precursor protein (APP) in brain of AD model using Drosophila melanogaster.
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Li L, Xu JL, Ma ZL, Dong MJ, Peng Y, et al., 2013. ‘The large-leaved Kudingcha (Ilex latifolia Thunb and Ilex kudingcha C.J. Tseng): a traditional Chinese tea with plentiful secondary metabolites and potential biological activities’. J Nat Med 67: 425-437.
Kim JY, Jeong HY, Lee HK, Yoo JK, Bae.K., et al., 2011. ‘Protective effect of Ilex latifolia, a major component of “kudingcha”, against transient focal ischemia-induced neuronal damage in rats’. J Ethnopharmacol: 558–564.
Kim YJ, Lee KL, Jang YJ, 2015. ‘Ilex latifolia prevents amyloid β protein (25–35)-induced memory impairment by inhibiting apoptosis and tau phosphorylation in mice’. J Med Food 18: 1317–1326.
Kim JY, Lee HK, Hwang BY, Kim SH, Yoo JK, et al., 2012. ‘Neuroprotection of Ilex latifolia and caffeoylquinic acid derivatives against excitotoxic and hypoxic damage of cultured rat cortical neurons’. Arch Pharmacal Res 35: 1115–1122.
Le TX, Nguyen HA, Pham TNH, Nguyen VT, Nguyen TP, et al., 2015. ‘Antidepressant-like effect of Ocimum sanctum in Olfactory Bulbectomized mice’. Tạp chí dược liệu 20 311-316.
Le XT, Pham HT, Do PT, Fujiwara H, Tanaka K, et al., 2013. ‘Bacopa monnieri ameliorates memory deficits in olfactory bulbectomized mice: possible involvement of glutamatergic and cholinergic systems’. Neurochem Res 38: 2201-2215.
Yamada M, Hayashida M, Zhao Q, Shibahara N, Tanaka K, et al., 2011. ‘Ameliorative effects of yokukansan on learning and memory deficits in olfactory bulbectomized mice’. Journal of Ethnopharmacology 135: 737-
746.
Phillips RG, LeDoux JE, 1992. ‘Differential contribution of amygdala and hippocampus to cued and contextual fear conditioning’. Behav Neurosci 106: 274-285.
Oba A, Nakagawasai O, Onogi H, Nemoto W, Yaoita F, et al., 2013. ‘Chronic fluvoxamine treatment changes 5-HT(2A/2C) receptor-mediated behavior in olfactory bulbectomized mice’. Life Sci 92: 119-124.
Bahar-Fuchs A, Chetelat G, Villemagne VL, Moss S, Pike K, et al., 2010. ‘Olfactory deficits and amyloid- burden in Alzheimer's disease, mild cognitive impairment, and healthy aging: a PiB PET study’. J Alzheimers Dis 22: 1081-1087.
Bahar-Fuchs A, Moss S, Rowe C, Savage G, 2010. ‘Olfactory performance in AD, aMCI, and healthy ageing: a unirhinal approach’. Chemical Senses 35: 855-862.
Aleksandrova IY, Kuvichkin VV, Kashparov IA, Medvinskaya NI, Nesterova IV, et al., 2004. ‘Increased level of beta-amyloid in the brain of bulbectomized mice’. Biochemistry (Mosc) 69: 176-180.
Hozumi S, Nakagawasai O, Tan-No K, Niijima F, Yamadera F, et al., 2003. Characteristics of changes in cholinergic function and impairment of learning and memory-related behavior induced by olfactory bulbectomy. Behavioural Brain Res 138: 9-15.
Sithisarn P, Rojsanga P, Jarikasem S, Tanaka K, Matsumoto K, 2013. ‘Ameliorative effects of Acanthopanax trifoliatus on cognitive and emotional deficits in olfactory bulbectomized mice: An animal model of depression and cognitive deficits’. Evid Based Complement Alternat Med 2013: 701956.
Csernansky JG, Martin M, Shah R, Bertchume A, Colvin J, et al., 2005. ‘Cholinesterase inhibitors ameliorate behavioral deficits induced by MK-801 in mice’. Neuropsychopharmacol 30: 2135-2143.
DOI: https://doi.org/10.22146/mot.53686
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Universitas Gadjah Mada