Combination Effects of Moringa oleifera Leaf Ethanol Extract and Andrographis paniculata Herb on Blood Glucose Levels and Pancreas Histopathology of Diabetic Rats Induced by Streptozotocin

https://doi.org/10.22146/mot.39401

Annisa Fatmawati(1*), Moch. Saiful Bachri(2), Laela Hayu Nurani(3)

(1) Pharmaceutical Study Program, Alma Ata University, Yogyakarta
(2) Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
(3) Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta
(*) Corresponding Author

Abstract


Type 2 diabetes mellitus is a non-contagious disease, can damage the glucose metabolic system in the body, and is characterized by hyperglycemic conditions. Moringa leaves (Moringa oleifera) and Andrographis herbs (Andrographis paniculata) have been studied to have antihyperglycemic activity in diabetic rats. The purpose of this study was to determine the effect of the combination of Moringa leaf ethanol extract (MLEE) and ethanol extract of Andrographis herbs (AHEE) in streptozotocin-induced rats (45 mg/kgBW). The subjects consisted of 32 rats, divided into 8 groups (1 healthy group and 7 type 2 DM groups). The study was conducted for 4 weeks by measuring blood glucose levels in pre-treatment, day 0, 14 and 28 with a single MLEE treatment dose of 300 mg/kgBW, single AHEE 300 mg/kgBW, combination of MLEE and AHEE 150+150 mg/kgBW, 200+100 mg/kgBW, 100 + 200 mg/kgBW, and gliclazide 5 mg/kgBW orally. The results of measurement of fasting blood glucose levels on day 28 showed that administration of gliclazide 5 mg/kg BW, single dose MLEE and AHEE, as well as its combination, had significant differences (p<0.05) compared to the hyperglycemic control group. Pancreatic organ histopathology features in the extract dose group showed that there was a change in the repair of insula Langerhans compared to the hyperglycemic control group which showed necrotic damage due to streptozotocin induction. Combination administration has the same antihyperglycemic effect by single dose extract in diabetic rats within 28 days, which also restore weight loss to normal.


Keywords


Moringa oleifera; Andrographis paniculata; type 2 diabetes mellitus; pancreatic ß cell histopathology

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References

Adriawan, Ignatius Ryan, Mohamad Andrie, Rina Susilowati, Suwidjiyo Pramono and Agung Endro Nugroho. 2014. Evaluasi Efek Anti-Diabetes Melitus Ekstrak Terpurifikasi Andrographis paniculata (Burm.f.) Nees dan Andrografolid Dengan Parameter Indeks HOMA-IR. Trad. Med. J., January 2014 Vol. 19(1), p 19-23 ISSN : 1410-5918

Ali, Fahmy T., Nahla S. Hassan dan Rehab R. Abdrabou. 2015. Potential activity of Moringa Oleifera leaf extract and some active ingredients against diabetes in rats. International Journal of Scientific & Engineering Research, Volume 6, Issue 5

Anyanwu, Anthony Chinedu., Salako Olanrewaju Alani., Adeyemi Olufunmi Olaide. 2014. Effect of the Ethanolic Leaf Extract of Moringa oleifera on Insulin Resistance in STZ Induced Diabetic Rats. Journal of Plant Sciences. Vol. 2, No. 6-1, 2014, pp. 5-12.

Burhanuddin, Marianti A. Manggau, Faisal Attamimi. 2014. Combined Effect Extract Leaves of Moringa (Moringa Oleifera) and Sambiloto Herbs (Andrographis Paniculata) Blood Glucose Levels Lowering Male Mice (Mus Musculus) Effects of Dexamethasone. JST Kesehatan Januari 2014, Vol.4 No.1 : 17 – 24

Goud, Busineni Jayasimha. 2015. “Streptozotocin - A Diabetogenic Agent in Animal Models”. IJPPR.Human, 2015; Vol. 3 (1): 253-269.

Gupta, Arti, Navin R Sheth, Sonia Pandey and Jitendra Singh Yadav. 2015. Determination of Quercetin a Biomarker in Hepatoprotective Polyherbal Formulation through HPLC. J Chromatogr Sep Tech 2015, 6:6

Jangir, Ram Niwas dan Gyan Chand Jain. 2016. Antidiabetic And Antioxidant Potential Of Hydroalcoholic Extract Of Moringa oleifera Leaves In STZ-Induced Diabetic Rats. ejpmr, 2016,3(9), 438-450

Nesti, Dela Ria. 2015. Morfologi, Morfometri Dan Distribusi Sel Imunoreaktif Insulin Dan Glukagon Pada Pankreas Tikus (Rattus norvegicus) Obesitas. Tesis, Faculty of Veterinary Medicine, Gadjah Mada University.

Okhuarobo Agbonlahor, Joyce Ehizogie Falodun, Osayemwenre Erharuyi, Vincent Imieje, Abiodun Falodun, Peter Langer. 2014. Harnessing the medicinal properties of A. paniculata for diseases and beyond: a review of its phytochemistry and pharmacology. Asian Pac J Trop Dis 2014; 4(3): 213-222

Premanath, Ramya & Lakshmidevi Nanjaiah. Antidiabetic and Antioxidant potential of A. paniculata Nees. Leaf ethanol extract in STZ induced diabetic rats. J App Pharm Sci, 2015; 5 (01): 069-076.

Sarkar, Ananya, Ajay Tiwari, Parminder S. Bhasin and Moloy Mitra. 2011. Pharmacological and Pharmaceutical Profile of gliclazide: A Review. 2011. JAPS 01 (09); 2011: 11-19

Saravanan, Ganapathy and Ponnusamy Ponmurugan. 2013. S-allylcysteine Improves STZ-Induced Alterations of Blood Glucose, Liver Cytochrome P450 2E1, Plasma Antioxidant System, and Adipocytes Hormones in Diabetic Rats. Int J Endocrinol Metab. 2013 Oct; 11(4)

Sari, K. R. P., Sudarsono and Nugroho, A. E. Effect of herbal combination of Andrographis paniculata (Burm.f) Ness and Gynura procumbens (Lour.) Merr ethanolic extracts in alloxan-induced hyperglycemic rats. IFRJ 22(4): 1332-1337 (2015)

Soliman GZA. Anti-diabetic activity of dried Moringa oleifera leaves in normal and streptozotocin (Stz)-induced diabetic male rats. Indian J Appl Res, 2013; 3(9): 18-23.

Wibudi, Aris. 2006. Mekanisme Kerja Sambiloto (Andrographis paniculata) Sebagai Antidiabetes. Disertasi. Departemen Biologi, Sekolah Pasca Sarjana, Institut Pertanian Bogor.

Vinayagam, Ramachandran dan Baojun Xu. 2015. Antidiabetic properties of dietary flavonoids: a cellular mechanism review. Nutrition & Metabolism (2015) 12:60. Bio Med Central.



DOI: https://doi.org/10.22146/mot.39401

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