In Vitro α-Glucosidase Inhibitory Activity of Ethanol Extract of Buas-buas (Premna serratifolia Linn)

https://doi.org/10.22146/tradmedj.27917

Dini Hadiarti(1*)

(1) Department of Chemistry Education, Universitas Muhammadiyah Pontianak
(*) Corresponding Author

Abstract


In 2008, diabetics in Indonesia has reached 8,5 million of 11,1 % prevalence in West Kalimantan. It was estimated to reach 14,1 million in 2035. The treatment of diabetes may occur adverse reactions such as hypoglycemia, lipoatrophy, lipohypertrophy, lactic acidosis, gastrointestinal disturbances, allergic reactions, and obesity. Therefore, it is necessary to find an alternative medicine to overcome this problem. Buas-buas (Premna serratifolia Linn) could supposedly be an anti diabetic by inhibiting the α-glucosidase enzyme, due to the compositions of secondary metabolites, cardioprotective activity and it has a similar genus with Premna serratifolia Linn. The soxhlet extraction result from Premna serrtifolia Linn leaf powders produced of 34,1 % yield. Meanwhile, the activity of α-glucosidase in vitroinhibition test with amicroplate reader in various concentrations of samples of 0,125, 0,5, 1, 1,5, 2, dan 2,5 2% (b/v) were obtained the absorptions of 37,95, 74,77, 86,15, 91,03 and 91,69 %, respectively. The extraction of Premna serratifolia Linn leaf revealed that the concentration of 2 % of sample inhibited in vitro α-glucosidase with a percentage of 91,03 %. The extraction of Premna serratifolia Linn leafinhibited α-glucosidase of 97 % from the percentage of Acarboseinhibition.

Keywords


Inhibasi α-glukosidase; in vitro; Premna serratifolia Linn; Inhibition of α-glukosidase; in vitro; Premna serratifolia Linn

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References

ADA (American Diabetes Association). 2012. Diagnosis and Classification of Diabetes Mellitus. Diabetes Care. 35: 64-71.

BPOM (Badan Pengawas Obat dan Makanan). 2009. Diabetes mellitus. Informasi Produk Terapetik. 19(1): 1-12.

Depkes RI (Departemen Kesehatan Republik Indonesia). 2008. Laporan Nasional Riset Kesehatan Dasar 2007. Jakarta: Depkes RI

Elin, EY, Suwendar & Ernita Ekawati, 2006, Aktivitas Ekstrak Etanol Herba Seledri (Apium graveolens) dan Daun Urang Aring (Eclipta prostate L.) Terhadap Pityrosporum ovale. Majalah Farmasi Indonesia. 17(3):1-7.

Hartika R. 2009. Aktivitas Inhibasi α-glucosidase Ekstrak Senyawa Flavanoid Buah Mahkota Dewa. Skripsi. Bogor : Departemn Kimia FMIPA IPB.

Hollander P, Pi-Sunyer X, Conifff RF. 1997. Acarbose in the treatment of type 1 diabetes. Diabetes Care. 20 : 248-253.

Kavishankar, GB., Lakshimidevi, N., Murthy, S.M., Prakash, H.S., Niranjana, S.R.. 2011. Diabetes and medical plants-A review. Int J Pharm Biomed Sci. 2: 65-80.

Kawabata, J., Mizuhatta, K., Sato, E., Nishihoka, T., Aoyama, Y., & Kasai, T. 2003. 6-Hyroxyflavanoids as alpha-glucosidase inhibitors from marjoram (Origanum majorana) leaves. Bioscience, Biotechnology, and Biochemistry. 67(2): 445-447.

Li, L., Wang, Z., Ying, X., Mao, J., Sun, T., Tian, J. H., & Yang, K. 2012. Garlic for diabetes mellitus. Cochrane Database of Systematic Reviews. (7).

Majumder R, Akter S, Naim Z, Al-Amin M and Alam MB. 2014. Antioxidant and Anti-Diabetic Activities of the Methanolic Extract of Premna integrifolia Bark. Advances in Biological Research. 8 (1): 29-36.

Nagmoti, D dan Archana R. 2013. In Vitro Inhibitory Effects of Pithecellobium dulce (Roxb.) Benth. Seeds on Intestial Alpha-glucosidase and Pancreatic Alpha-amylase. Journal Biochemistry Technology. 4 (3): 616-621.

Pianto, M. S., de Carvalho, J.E., Lajolo, F. M., Genvese, M. I., and Shetty, K. 2012. Evalution of antiproliferative, anti-type 2 diabetes, and anthihypertension potentials of ellagitannins from strawberries (FRagraria ananassa Duch.) using in vitro models. Journal of Medical Food. 13(5): 27-35.

Purwatresna E. 2012. Aktivitas Antidiabetes Ekstrak Air Dan Etanol Daun Sirsak Secara In Vitro Melalui Inhibisi Enzim Α-Glukosidase. Skripsi. Bogor: Institut Pertanian Bogor.

Rajendran R and Basha NS. 2008 Cardioprotective effect of ethanol extract of stem-bark and stem-wood of Premna serratifolia Lin., (Verbenaceae). Research J. Pharm. and Tech. 1(4):7-14.

Rajendran R, Suseela L, Meenakshi Sundaram R and Saleem Basha N. 2008. Cardiac stimulant activity of bark and wood of Premna serratifoliaL. A. J. of the Bangladesh pharmalogical society. 3:107-113.

Rizki PR, Jayanti RD, dan Widyaningsih TD. 2015. Pengaruh Teh Herbal Berbasis Daun Cincau Hijau (Premna Oblongifolia Merr.) Terhadap Glukosa Darah Dan Profil Lipid Tikus Hiperglikemia. Jurnal Pangan dan Agroindustri. 3(3): 803-814.

Sigh CR, Nelson R, Krishnan PM, and Mahesh K and Pargavi B. 2011. Identification of Volatile Constituents from Premna serratifoliaL.through GC-MS. International Journal of PharmTech Research. 3(2) :1050-1058.

Sugiwati S, Setiasih S, Afifah E. 2009. Antihyperglycemic activity of the mahkota dewa [Phaleria macrocarpa (scheff.) boerl.] leaf extracts as an alpha-glucosidase inhibitor. Makara kesehatan. 13(2): 74-78.

Vadivu R, Jerad suresh A, Girinath K, Boopathi kannan P, vimala R and Sathish Kumar NM. 2009. Evaluation of Hepatoprotective and in-vitro cytotoxic activity of leaves of premna serratifolia L. J. of Scientific research. 1(1): 145-152.

Wahyuni S, Mukarlina, dan Yanti AP. 2014. Aktivitas Antifungi Ekstrak Metanol Daun Buas-Buas (Premna serratifolia) Terhadap Jamur Diplodia sp. Pada Jeruk Siam (Citrus nobilis var. microcarpa). Jurnal Protabiont. 3(2) : 274-–279.

Xu, H. 2010. Inhibition Kinetics of Flavanoids on Yeast α-glucosidase Merged with Docking Simulation. Protein and Peptide Letters. 17 (10): 1270-1279



DOI: https://doi.org/10.22146/tradmedj.27917

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