Enhancement of Delignification and Glucan Content of Sugarcane Bagasse by Alkali Pretreatment for Bioethanol Production

https://doi.org/10.22146/ajche.59093

Kyaw Wunna(1*), Kiohiko Nakasaki(2), Joseph Auresenia(3), Leonila Abella(4), Peg-asa Gaspilo(5)

(1) Department of Industrial Chemistry, Yadanabon University, Mandalay, Myanmar
(2) Department of International Development Engineering, Tokyo Institute of Technology, Tokyo, Japan
(3) Chemical Engineering Department, De La Salle University-Manila, Manila, Philippines
(4) Chemical Engineering Department, De La Salle University-Manila, Manila, Philippines
(5) Chemical Engineering Department, De La Salle University-Manila, Manila, Philippines
(*) Corresponding Author

Abstract


The current work aimed to enhance the delignification of sugarcane bagasse (SCB) for bioethanol production. The optimization of alkali (sodium hydroxide) pretreatment parameters such as concentration and residence time was carried out by the Taguchi method using L16 orthogonal array with two factors and four levels. Sugarcane bagasse powder was mixed with sodium hydroxide (NaOH) solution (0.5-2 wt.%) and heated in an autoclave at 121°C and at varied times (30-120 min). From the statistical analysis of data, it was observed that delignification and glucan increased with the increased concentration and short time. The optimum parameters of NaOH pretreatment were 2 wt.% of NaOH concentration and 30 minutes of residence time. At the optimum conditions, 86.8% delignification and 46.6% glucan content of SCB were obtained. Thus, alkali pretreatment optimized by Taguchi design is the effective method to remove lignin and to increase cellulose or glucan content in sugarcane bagasse for facilitating the further catalytic hydrolysis in bioethanol production.


Keywords


Delignification, Glucan, Lignin, Sugarcane bagasse, Alkali pretreatment

Full Text:

PDF


References

  1. Asgher, M., Ahmad, Z., Iqbal, H.M.N. (2013). "Alkali and enzymatic delignification of sugarcane bagasse to expose cellulose polymers for saccharification and bioethanol production," Inds. Crops & Products, 44, 488-495.
  2. Calvo-Flores F. G., Dobado, J. A., Isac-Garcia, J., Martin-Martinez, F. J. (2015). Lignin and lignans as renewable raw materials, John Wiley & Sons, New York, U.S.A.
  3. Canilha, L., Santos, V.T.O., Rocha, G.J.M., Almeida e Silva, J.B., Silva, S.S., Felipe, M.G.A., Ferraz, A., Milagres A.M.F., Carvalho W. (2011). "A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid," J. Ind. Microbiol. Biotechnol, 38, 1467-1475.
  4. Darvishi, F., and Moghaddami N. A. (2019). "Optimization of an industrial medium form molasses for bioethanol production using the Taguchi statistical experimental-design method," Fermentation, 5, 14.
  5. Das, S. P., Gupta, A., Das, D., Goyal, A. (2016). "Enhanced bioethanol production from water hyacinth (Eichhornia crassipes) by statistical optimization of fermentation process parameters using Taguchi orthogonal array design," Int. Biodet. & Biodegr., 109, 174-184.
  6. Gao Y., Xu J., Zhang Y., Yu Q., Yuan Z., Liu Y. (2013). "Effects of different pretreatment methods on chemical composition of sugarcane bagasse and enzymatic hydrolysis," Bioresour. Technol., 144, 396-400.
  7. Hosgun, E. Z., Berikten, D., Kivanc, M., Bozan B. (2017). "Ethanol production from hazelnut shell through enzymatic saccharification and fermentation by low-temperature alkali pretreatment," Fuel, 196, 280-287.
  8. Jonglertjunya W., Juntong, T., Pakkang, N., Srimarut, N., Sakdaronnarong C. (2014). "Properties of lignin extracted from sugarcane bagasse and its efficacy in maintaining postharvest quality of limes during storage," LWT – Food Sci. & Technol., 57, 116-125.
  9. Ju, Y. H., Huynh, L. H., Kasim N. S., Guo, T. J., Wang J. H., Fazary, A. E. (2011). "Analysis of soluble and insoluble fractions of alkali and subcritical water treated sugarcane bagasse," Carbohydr. Polym, 83, 591-599.
  10. Jung, W., Savithri, D., Sharma-Shivappa, R., Kolar P. (2018). "Changes in lignin chemistry of switchgrass due to delignification by sodium hydroxide pretreatment," Energies, 11, 276.
  11. Kim, J. S., Lee, Y. Y., Kim, T. H. (2016). "A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass," Bioresour. Technol., 199, 42-48.
  12. Liu, H., Pang, B., Zhou, J., Han, U., Lu, J., Li, H. (2016). "Comparative study of pretreated corn stover for sugar production using cotton pulping black liquor (CBPL) instead of sodium hydroxide," Ind. Crops Prod., 84, 97-103.
  13. Long, J., Li, X., Guo, B., Wang, L., Zhang N. (2013). "Catalytic delignification of sugarcane bagasse in the presence of acidic ionic liquids," Catalysis Today, 200, 99-105.
  14. Palmqvist, E., Hahn-Hagerdal, B. (2000). "Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition," Bioresour. Technol., 74, 25-33.
  15. Radhakumari, M., Ball, A., Bhargava, S. K., Satyavathi, B. (2014). "Optimization of glucose formation in karanja biomass hydrolysis using Taguchi robust method," Bioresour. Technol., 166, 534-540.
  16. Rocha, G. Jackson de Moraes, Martin C., Soares, I. B., Maior, A. M. S., Baudel, H. M., Moraes de Abreu, C. A. (2011). "Dilute mixed-acid pretreatment of sugarcane bagasse for ethanol production," Biomass & Bioenergy, 35, 663-670.
  17. Singh, R., Shukla, A., Tiwari, S., Srivastava, M. (2014). "A review on delignification of lignocellulosic biomass for enhancement of ethanol production potential," Renewab. Sust. Ener. Reviews, 32, 713-728.
  18. Sluiter A., Hames B., Ruiz R., Scarlata C., Sluiter J., Templeton D., Crocker D. (2011). "Determination of structural carbohydrates and lignin in biomass," Technical Report, NREL/TP-510-42618, Colorado, United States.
  19. Wang, Q., Wang, W., Tan, X., Zahoor, Chen, X., Guo, Y., Yu, Q. (2019). "Low-temperature sodium hydroxide pretreatment for ethanol production from sugarcane bagasse without washing process," Bioresour. Technol., 291, 121844.
  20. Wang, W., Wang X., Zhang, Y., Yu, Q., Tan, X., Zhuang X., Yuan, Z. (2020). "Effect of sodium hydroxide pretreatment on physicochemical changes and enzymatic hydrolysis of herbaceous and woody lignocelluloes," Ind. Crops Prod., 145, 112145.
  21. Wunna, K., Nakasaki, K., Auresenia, J., Abella L., Gaspillo Pag-asa D. (2017). "Effect of alkali pretreatment on removal of lignin from sugarcane bagasse," Chem. Eng. Transc., 56, 1831-1836
  22. Xu, F., Shi, Y. C., Wang, D. (2012). "Structural features and changes of lignocellulosic biomass during thermochemical pretreatments: a synchrotron X-ray scattering study on photoperiod-sensitive sorghum," Carbohydr. Polym, 88, 1149–1156.



DOI: https://doi.org/10.22146/ajche.59093

Article Metrics

Abstract views : 3321 | views : 3108

Refbacks

  • There are currently no refbacks.


ASEAN Journal of Chemical Engineering  (print ISSN 1655-4418; online ISSN 2655-5409) is published by Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada.