Microwave-Assisted Extraction of Polyphenol Content from Leaves of Tristaniopsis merguensis Griff.

https://doi.org/10.22146/ajche.50448

Robby Gus Mahardika(1*), Occa Roanisca(2)

(1) Department of Chemistry, Faculty of Engineering, Universitas Bangka Belitung
(2) Department of Chemistry, Faculty of Engineering, Universitas Bangka Belitung
(*) Corresponding Author

Abstract


Tristaniopsis merguensis Griff. is a species of the Myrtaceae family and has been widely used by people of Bangka Belitung as a traditional medicine to reduce cholesterol, gastric pains, and improve cardiac performance. Extraction methods are the crucial efficacy of herbal medicine. The conventional method, like maceration, takes a long time. In this study, the leaves of Tristaniopsis merguensis were extracted using Microwave-Assisted Extraction (MAE) to reduce extraction time. The extraction using MARS (Microwave Accelerated Reaction System) 6 by CEM Corporation with time variation times of 5, 10, 15, 30 min with temperature of 60, 80, 100oC at 1200 W. The yield using acetone extraction of Tristaniopsis merguensis leaves increases with time and temperature. The extraction dependent on solvent extraction, polar solvent like ethanol, and methanol were higher than semi-polar solvents like acetone and ethyl acetate. The polyphenol content of acetone extract using MAE (10 min, 80oC) was found to be 234.67 mg Gallic Acid Equivalent per gram (GAE/g); it was higher than acetone extract using maceration. The phytochemical results show there are no difference in the active compound using MAE and maceration, i.e. alkaloids, tannin, and flavonoids. Yield extraction, time, and phytochemical results of MAE are more favorable than a maceration.


Keywords


Gallic Acid Equivalent (GAE), Microwave-Assisted Extraction, Polyphenol content, Phytochemical, Tristaniopsis merguensis

Full Text:

PDF


References

  1. Bellosta, S., Dell`Agli, M., Canavesi, M., Mitro, N., Monetti, M., Crestani, M., Verrota, L., Fuzzati, N., Bermini, F., & Bosisio, E. (2003), "Inhibition of metalloproteinase-9 activity and gene expression by polyphenolic compounds isolated from the bark of Tristaniopsis calobuxus (Myrtaceae)." Cellular and Molecular Life Sciences CMLS 60 (7): 1440-1448.
  2. Bhuyan, D.J., Vuong, Q.V., Chalmers, A.C., van Altena, I.A., Bowyer, M.C., Scarlett, C.J. (2015), "Microwave-assisted extraction of Eucalyptus robusta leaf for the optimal yield of total phenolic compounds." Industrial Crops and Products 69: 1–10.
  3. Borja, J. Q., Uy, M. M., Lim, J. S., Ong, M. E., & Ros, A. M. (2014). Microwave – Assisted Extraction of Chlorogenic Acid from Coffee liberica L. ASEAN Journal of Chemical Engineering, 14(2), 58–66.
  4. Carocho, M., Barreiro, M. F., Morales, P., & Ferreira, I. C. F. R. (2014), " Adding molecules to food, pros, and cons: A review of synthetic and natural food additives." Comprehensive Reviews in Food Science and Food Safety 13: 377-399.
  5. Chigurupati, S., Yiik, E.W.K., Mohammad, J.I., Vijayabalan, S., Selvarajan, K.K., Reddy, V.R., Nanda, S.S. (2018), "Screening antimicrobial potential for malaysian originated Tamarindus Indica Ethanolic Leaves Extract." Asian Journal of Pharmaceutical and Clinical Research 11 (3): 361-363.
  6. Dahmoune, F., Nayak, B., Moussi, K., & Remini, H. (2015), "Optimization of microwave-assisted extraction of polyphenols from Myrtus communis L. leaves." Food Chemistry 166: 585-595.
  7. del Mundo, C. I., Cavarlez, J. M., Pe, A. M., & Roces, S. (2018). Microwave Assisted Glycerolysis Of Neem Oil. ASEAN Journal of Chemical Engineering, 18(1), 17–23.
  8. El-Ghorab, A.H., Nauman, M., Anjum, F.M., Hussain, S., Nadeem, M. (2010), "A comparative study on chemical composition and antioxidant activity of ginger (Zingiber officinale) and Cumin (Cuminum cyminum)." Journal Agricultural and Food Chemistry 58: 8231-8237.
  9. Hemwimon, S., Pavasant, P., & Shotipruk, A. (2007), "Microwave-Assisted Extraction of antioxidative anthraquinones from roots of Morinda citrifolia." Separation Purification Technology 54: 44-50.
  10. Hendra, R., Ahmad, S., Oskoueian, E., Sukari, A., & Shukor, M.Y. (2011), "Antioxidant, anti-inflammatory and citotoxicity of Phaleria macrocarpa (Boerl.) Scheff Fruit." Journal of the International Society for Complementary Medicine Research 11: 110-121.
  11. Hoa, J., Huang, S., Han, W., & Xue, B. (2002), "Microwave-Assisted Extraction of artemisinin from Artemisia annua L." Separation and Purification Technology 28: 191-196.
  12. Kaufmann, B., & Christen, P. (2002). Recent Extraction Techniques for Natural Products : Microwave-assisted Extraction and Pressurised Solvent Extraction. Phytochemcal Analysis, 113(2), 105–113.
  13. Kim, J. (2017). Extraction time and temperature a ff ect the extraction e ffi ciencies of coumarin and phenylpropanoids from Cinnamomum cassia bark using a microwave-assisted extraction method. Journal of Chromatography B, 1063(August), 196–203.
  14. Kusmardiyani, S., Novita, Grace., Fidrianny, I. (2016), "Antioxidant activities from various extracts of different parts of kelakai (Stenochlaena palustris) in central Kalimantan-Indonesia." Asian Journal of Pharmaceutical and Clinical Research 9: 215-219.
  15. Lasano, N.F., Rahmat, A., Ramli, N.S., & Bakar, M.F.A. (2018), "Effect of oven and microwave drying on polyphenols content and antioxidant capacity of herbal ea from strobilanthes crispus leaves." Asian Journal of Pharmaceutical and Clinical Research 11 (6): 363-368.
  16. Li, Y., Fabiano-Tixier, A., & Vian, M., A. (2013), "Solvent-free microwave extraction of bioactive compound provides a tool for green analitical chemistry." Trends in Analytical Chemistry 47: 1-11.
  17. Lovrić, V., Putnik, P., Kovačević, D. B., Jukić, M., & Dragović-uzelac, V. (2017). Effect of Microwave-Assisted Extraction on the Phenolic Compounds and Antioxidant Capacity of Blackthorn Flowers. Food Technol. Biotechnol, 55(2), 243–250.
  18. Mahardika, R. G., & Roanisca, O. (2018). Antioxidant Activity And Phytochemical Of Extract Ethyl Acetat Pucuk Idat (Cratoxylum glaucum). Indo. J. Chem. Res, 5(2), 481–486.
  19. Mandal, V., Mohan, Y., Hemalatha, S. (2007), "Microwave Assisted Extraction- An Innovative and Promosing Extraction Tool For Medicinal Plant Research." Pharmaconosy Reviews 1 (1): 7-18.
  20. Roanisca, O., Mahardika, R. G., & Setiawan, Y. (2019). Tristaniopsis merguensis Griff. Extract as Inhibitor for Corrosion of Stainless Steel. IOP Conference Series: Earth and Environmental Science, 353 012020.
  21. Routray, W., & Orsat, V. (2012). Microwave-Assisted Extraction of Flavonoids : A Review. Food and Bioprocess Technology, 5, 409–424.
  22. Safdar, M., N., Kausar, T., Jabaar, S., Mumtaz, A., Ahad, K., & Saddozai., A.A.,. (2016), "Extraction and quantification of polyphenols from kinnow (Citrus reticulate L.) peel using ultrasound and maceration techniques." Journal of Food and Drug Analysis 25: 488-500.
  23. Sporring, S., Bøwadt, S., Svensmark, B., & Bjorklund, E. (2005), "Comprehensive comparison of classic Soxhlet extraction with Soxtec extraction, ultrasonication extraction, supercritical fluid extraction, microwave assisted extraction and accelerated solvent extraction, for the determination of polychlorinated biphenyls." Journal Chramatography A 1090: 1-9.
  24. Sukandar, E.Y., Kurnita, N.F., Wikaningtyas, P., & Agprikani, D. (2016), "Antibacterial interaction of combination of ethanolic extract of Zingiber Officinale Var Rubrum rhizome, Boesenbergia pandurata rizhome, and Stevia rebaudiana leaves certain antibiotics against infectious mouth microbial." Asian Journal of Pharmaceutical and Clinical Research 9 (1): 332-335.
  25. Verotta, L., Dell`Agli, M., Giolito, A., Guerrini, M., Cabalion, P., Bosisio, E. (2001), "In Vitro Antiplasmodial Activity of Extracts of Tristaniopsis Species and Identification of the Active Constituents: Ellagic Acid and 3,4,5-Trimethoxyphenyl-(6`-O-galloyl)-O-Betha-D-glucopyranoside." J.Nat.Prod 64: 603-607.
  26. Wang, H., & Helliwell, K. (2001). Determination of ¯ avonols in green and black tea leaves and green tea infusions by high-performance liquid chromatography. Food Research International, 34, 223–227.
  27. Wang, Y., You, J., Yu, Y., Qu, C., Zhang, H., Ding, L., … Li, X. (2008). Analysis of ginsenosides in Panax ginseng in high pressure microwave-assisted extraction. Food Chemistry, 110(1), 161–167.
  28. Xiao, W., Han, L., & Shi, B. (2008). Microwave-assisted extraction of flavonoids from Radix Astragali. Separation and Purification Technology, 62(3), 614–618.
  29. Yap, Rahmani M., Taufiq Y.H. (2007), "Compounds from Cratoxylum aborescens, Cratoxylum glaucum, Garcinia nitida and Garcinian mangostana and their Potential as Anti-Cancer Lead Compounds." Pertanika Journal of Science & Technology 1 (15): 43-47.
  30. Yarli, N. (2011), Ekologi pohon pelawan (Tristaniopsis merguensis Griff.) sebagai Inang Jamur Pelawan di Kabupaten Bangka Tengah. Bogor: Sekolah Pascasarjana Institut Pertanian Bogor.



DOI: https://doi.org/10.22146/ajche.50448

Article Metrics

Abstract views : 6563 | views : 5644

Refbacks

  • There are currently no refbacks.


ASEAN Journal of Chemical Engineering  (print ISSN 1655-4418; online ISSN 2655-5409) is published by Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada.