Hydrogen Production from Synthesis Gas Using the Photosynthetic Bacterium Rhodospirillum rubrum

https://doi.org/10.22146/ajche.50162

Ghasem Najafpour(1*), Habibollah Younesi(2)

(1) Department of Chemical Engineering, Engineering College University of Mazandaran, Shariati Ave., Babol, IRAN
(2) School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia Seri Ampangan, 14300 Nibong Tebal, Penang, MALAYSIA
(*) Corresponding Author

Abstract


Production of biological hydrogen by anaerobic photosynthetic bacteria, specifically Rhodospirillum rubrum, from synthesis gas was successfully conducted at ambient temperature and pressure. The influence of initial acetate concentration as the substrate for microbial growth was investigated in a batch system. Series of experiments were conducted using serum bottles as bioreactor. The agitation rate and light intensity were adjusted at 200 rpm and 1,000 lux, respectively. The concentration of acetate as carbon source was varied from 0.5 to 3.0 g/I. It was observed that the increase in concentration of the carbon source from 2.5 to 3 g/l resulted in the decrease both in the growth of the microorganism and in hydrogen production rate. Experimental results showed that the optimum acetate concentration would be from 1 to 2 g/I. The resulting data also showed that in 1-2 g/I acetate, highest hydrogen formation and cell concentration were obtained. Additional acetate in the initial culture medium inhibited the growth of R. rubrum. An inverse relationship between acetate concentration and initial cell growth was observed. This article presents a method to calculate the mass transfer coefficient for gaseous substrates and the process parameters involved in a gas and liquid fermentation system. The procedure had been defined by experimental data for the bioconversion of CO to C02' while H20 is converted into hydrogen. Hence, a biologically-based water-gas shift reaction provided an attractive alternative improvement for renewable resources to achieve higher hydrogen production. Keywords: Anaerobic bacteria, batch culture, coefficient, CO uptake rate, mass transfer, photobiological hydrogen, and Rhodospirillum rubrum.

Keywords


Anaerobic bacteria, batch culture, coefficient, CO uptake rate, mass transfer, photobiological hydrogen, and Rhodospirillum rubrum.

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References

  1. Baily, J. E., and Ollis, D. F. (1986). Biochemical engineering fundamentals, McGraw-Hill, New York, 394-408.

  2. Barbosa, M. J., Rocha, J. M. S., Tramper, J., and Wijffels, R.H. (2001). "Acetate as a carbon source for hydrogen production by photosynthetic bacteria," J. of Biotechnol., 85, 26-33.

  3. Feik, C., French, R., Czernik, S. and Chornet, E. (2000). "Production of hydrogen from biomass-derived liquids," Solar Engineering, 82, 87–93.

  4. Ghirardi, M.L., Kosourov, S., Tsygankov, A., and Seibert, M. (2000). "Two-phase photobiological agal Hy-production system." Proceedings of the 2000 DOE Hydrogen Program Review, NREL/CP 570-28890.

  5. Hoek, P. V., Aristidou, A., Hanhn, J.J., and Patist, A. (2003). “Fermentation goes large scale," Biotechnology CEP, 37S-41S.

  6. Jung, Y. G., Kim, J. R., Park, J. Y., and Park, S. (2002). "Hydrogen production by a new chemoheterotrophic bacterium Citrobacter sp. Y19," Int. J. of Hydrogen Energy, 27, 601-610.

  7. Maness, P. C., and Weaver, P. F. (1999). "Biological H, from fuel gases and from H,O." Proceedings of the 2000 U.S. DOE Hydrogen Program Review, NREL/CP 570-26938.

  8. Miyake, J., Miyake, M., and Asada, Y. (1999). “Biotechnological hydrogen production: Research for efficient light energy conversion," J. of Biotechnol., 70, 89–101.

  9. Najafpour, G. D., Younesi, H., Ku Ismail, K. S., Mohamed, A. R., and Kamaruddin, A.H. (2002). "Production of hydrogen from synthetic gas using Rhodospirillum rubrum, batch fermentation." Proceedings of the Regional Symposium on Chemical Engineering (RSCE 2002), 16th Symposium of Malaysia Chemical Engineers (SOMChE 2002), 28-30 October, Malaysia, 87–94.

  10. Nakada, E., Nishikata, S., Asada, Y., and Miyake, J. (1999). "Photosynthetic bacterium hydrogen production combined with a fuel cell," Int. J. of Hydrogen Energy, 24, 1053-1057.

  11. Schoert, H. H., and Song, C. (2002). "Chemicals and materials from coal in the 21st century," Fuel, 81, 15–32.

  12. Valentine, D. L., and Blanton, D. C. (2000). "Hydrogen production by methanogens under low-hydrogen conditions," Arch. MicroBiol., 174, 415-421.

  13. Wolfrum, E. J., Watt, A.S., and Huang, J. (2002). "Bioreactor development for biological hydrogen production." Proceedings of the 2002 DOE Hydrogen Program Review, NREL/CP-570-28890.

  14. Zhu, H., Wakayama, T., Asada, Y., and Miyake, J. (2001). "Hydrogen production by four cultures with participation by anoxygenic photosynthetic bacterium and anaerobic bacterium in the presence of NH +," Int. J. of Hydrogen Energy, 26, 1149-1154.



DOI: https://doi.org/10.22146/ajche.50162

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ASEAN Journal of Chemical Engineering  (print ISSN 1655-4418; online ISSN 2655-5409) is published by Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada.