The Effect of Metanolic Extract and Ethyl Acetate Fraction of Kepel Fruits to Lipid Peroxide Concentration in Liver of CCl4-induced Rat

Mitsalina Fildzah Arifah; Sudibyo Martono; Sugeng Riyanto; Claude Mona Airin

doi:10.22146/jfps.661

Mitsalina Fildzah Arifah Fakultas Farmasi, Universitas Gadjah Mada Yogyakarta
Sudibyo Martono Departemen Kimia Farmasi, Fakultas Farmasi, Universitas Gadjah Mada Yogyakarta
Sugeng Riyanto Departemen Kimia Farmasi, Fakultas Farmasi, Universitas Gadjah Mada Yogyakarta
Claude Mona Airin Departemen Anatomi dan Fisiologi Hewan, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta

Keywords: antioxidant, lipid peroxidation, MDA, meat of kepel fruit

Abstract

Accumulation lipid peroxidation can lead to the formation of malondialdehyde to generate
free radicals. Antioxidant will minimize free radical related health problems, such as natural
antioxidant from meat of kepel (Stelechocarpus burahol) (Blume) Hook. F. & Thompson) fruits.
Antioxidant activities of methanol extracts and acetate ethyl fractions meat of kepel fruits calculated
profile of malondialdehyde concentration (ng/mL) in CCl4 induced acute liver toxicity in female
Sprague Dawley rats using ELISA competitively method. All of groups induced CCl4 i.p. at 0 h, except
untreated and solvent control group, then vitamin C , methanol extracts and acetate ethyl fractions
p.o. once daily for 72 h. Identification of secondary metabolite was using phytochemical screening
and Thin Layer Chromatography (TLC). Liver weighed and calculated relative organ weight (ROW)
of liver, then all of data analyzed using statistic tests, One Way ANOVA and Kruskall Wallis. This
study showed methanol extracts can decrease MDA concentration, ALT and AST were more potent
(significantly p<0.05) than acetate ethyl fractions and vitamin C. The TLC analysis identified methanol
extracts have same polarity with quercetin (Rf = 0,26) and acetate ethyl fractions showed other
flavonoid class, except rutin and quercetin. ROW of acute liver toxicity in rats increased significantly
in CCl4 and vitamin C groups.