The resistance status of Aedes aegypti larvae to Temephos in Depok, Sleman, Yogyakarta

  • Mia Munawaroh Yuniyanti Department of Pharmacology and Therapy Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada
  • Sitti Rahmah Umniyati Department of Parasitology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Ernaningsih Department of Parasitology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

Abstract

There are still many cases of dengue hemorrhagic fever and tend to increase
over time. One strategy to reduce the increase in cases of dengue infection is
to eradicate Aedes aegypti as a vector using insecticides. The use of insecticides
for a long time can cause resistance. The purpose of this study was to determine
the resistance status of Aedes aegypti larvae against temephos in Depok, Sleman.
This quasi experimental test was carried out on 3rd instar larvae of aedes
aegypti from RW 9 and 10 Minomartani, Depok, Sleman. The treatment group
was exposed 0.02 ppm temephos for 24 hours. The analysis was presented in the
form of percentage of larval mortality and resistance categories based on WHO
guidelines. The mortality percentage of Aedes aegypti larvae in RW 9 was 100%,
while the mortality from RW 10 was 97%. Aedes aegypti larvae in Depok district,
Sleman are still susceptible to temephos at a dose of 0.02 ppm.

References

WHO. Dengue and Severe Dengue [Internet]. WHO. 2020 [cited 6 Feb 2021]. Available from: https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue

Kementerian Kesehatan RI. Data Kasus Terbaru DBD di Indonesia [Internet]. Kemenkes. 2021 [cited 10 Feb 2021]. Available at https://sehatnegeriku.kemkes.go.id/baca/umum/20201203/2335899/data-kasus-terbaru-dbd-indonesia/

Widjaja J. Survey entomologi Aedes sp pradewasa di dusun satu kelurahan Minomartani Kecamatan Depok Kabupaten Sleman Provinsi Yogyakarta. Aspirator 2012; 4(2):64-72.

Bowman LR, Donegan S, McCall PJ. Is dengue vector control deficient in effectiveness or evidence?: systematic review and meta-analysis. PLoS Negl Trop Dis 2016; 10(3):e0004551.

https://doi.org/10.1371/journal.pntd.0004551

World Health Organization. Comprehensive guidelines for prevention and control of dengue and dengue haemorrhagic fever: revised and expanded edition. WHO SEARO. 2012. India.

Rather IA, Parray HA, Lone JB, Paek WK, Lim J, Bajpai VK et al. Prevention and control strategies to counter dengue virus infection. Front Cell Infect Microbiol 2017; 7:336.

https://doi.org/10.3389/fcimb.2017.00336

Kementerian Kesehatan RI. Pedoman pencegahan dan pengendalian demam berdarah dengue di Indonesia. Direktorat Jenderal Pengendalian Penyakit dan Penyehatan Lingkungan Kemenkes RI. 2017. Jakarta.

Dusfour I, Vontas J, David J, Weetman D, Fonseca DM, Corbel V, et al. Management of insecticide resistance in the major Aedes vectors of arbo viruses: Advances and challenges. PLoS Negl Trop Dis 2019; 13(10):e0007615.

https://doi.org/10.1371/journal.pntd.0007615

WHO. Monitoring and managing insecticide resistance in Aedes mosquito populations: Interim guidance for entomologists. World Health Organization 2016. Geneva.

CDC. Guideline for evaluating insecticide resistance in vectors using the CDC bottle bioassay. Centers for Disease Control and Prevention 2010. Atlanta.

WHO. Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. World Health Organization 1981.

Kauffman E, Payne A, Franke MA, Schmid MA, Harris E, Kramer LD. Rearing of Culex spp. and Aedes spp. mosquitoes. Bio Protoc 2017; 7(17):e2542.

https://doi.org/10.21769/BioProtoc.2542

Melo-Santos MAV, Varjal-Melo JJM, Araujo AP, Gomes TCS, Paiva MHS, Regis LN, et al. resistance to the organophosphate temephos: Mechanisms, evolution and reversion in an Aedesaegypti laboratory strain from Brazil. Acta Tropica 2010; 113(2):180-9.

https://doi.org/10.1016/j.actatropica.2009.10.015

Marcombe S, Fustec B, Cattel J, Chonephetsarath S, Thammavong P, Phommavanh N, et al. Distribution of insecticide resistance and mechanisms involved in the arbovirus vector Aedesaegypti in Laos and implication for vector control. PLoS Negl Trop Dis 2019;1 3(12):e0007852.

https://doi.org/10.1371/journal.pntd.0007852

Grisales N, Poupardin R, Gomez S, Fonseca-Gonzalez I, Ranson H, Lenhart A. Temephos resistance in Aedesaegypti in Colombia compromises dengue vector control. PLoS Negl Trop Dis 2013; 7(9):e2438.

https://doi.org/10.1371/journal.pntd.0002438

Helvecio E, Romao TP, de Carvalho-Leandro D, de Oliveira IF, Cavalcanti AEHD, Reimer L, et al. Polymorphisms in GSTE2 is associated with temephos resistance in Aedesaegypti. Pestic Biochem Physiol 2020; 165:104464.

https://doi.org/10.1016/j.pestbp.2019.10.002

Published
2021-02-25
Section
Articles