Pigment Extraction Method for Anthocyanin Natural Resources in Indonesia: A Review
Abstract
Anthocyanin is the pigment that still attracts attention from food industries because of its abundance in Indonesian plants. Anthocyanins are produced in a variety of colors with unique stability properties. Through a systematic review, this review studied 40 scientific reports conducted in Indonesia over the last 10 years. The research objects included fruits, flowers, seeds, peels, and roots from the islands of Java, Sumatra, Kalimantan, Sulawesi, and Nusa Tenggara. Several methods are used to extract solvent-based anthocyanin compounds, including maceration, microwave, and reflux-soxhlet extraction. Microwave extraction, including ultrasonic extraction, is recommended for anthocyanin extraction. Spectrophotometry with the pH differential method was chosen as the anthocyanin quantification procedure, using 500 and 700 nm wavelenghts.
References
Achmad, Z., & Sugiarto, B., 2020. "Ekstraksi antosianin dari biji alpukat sebagai pewarnaan alami." Jurnal Teknologi Technoscientia 12(2), 134–143.
Agus, C., 2020. "Integrated bio-cycles system for sustainable and productive tropical natural resources management in indonesia. In Bioeconomy for Sustainable Development. Springer, Germany https://doi.org/10.1007/978-981-13-9431-7_11
Agustin, D., & Ismiyati, I., 2015. "Pengaruh konsentrasi pelarut pada proses ekstraksi antosianin dari bunga kembang sepatu." Jurnal Konversi 4(2), 9-16.
Alvionita, E. A., 2020. Pengaruh rasio bahan dan pelarut pada ekstraksi antosianin bunga dadap merah (Erythrina cristagali) menggunakan metode MAE (microwave assisted extraction). Thesis. Universitas Negeri Semarang.
Alvionita, J., Darwis, D., Darwis, D., & Efdi, M., 2016. "Ekstraksi dan identifikasi senyawa antosianin dari jantung pisang raja (Musa X paradisica L.) serta uji aktivitas antioksidannya." Jurnal Riset Kimia 9(2), 21-28.
Amperawati, S., Hastuti, P., Pranoto, Y., & Santoso, U., 2019. "Efektifitas frekuensi ekstraksi serta pengaruh suhu dan cahaya terhadap antosianin dan daya antioksidan ekstrak kelopak rosella (Hibiscus sabdariffa L.)." Jurnal Aplikasi Teknologi Pangan 8(1), 38–45.
Angelia, I. O., 2019. "Variasi konsentrasi solven pada proses ekstraksi antosianin dari ubi jalar ungu." Journal of Agritech Science 3(3), 16–26.
Arifin, A. A., Armiani, S., & Fitriani, H., 2022. "Isolasi antosianin kulit terong ungu (Solanum melongena) sebagai biosensor pendeteksi kandungan bahan kimia pada makanan." Bioscientist : Jurnal Ilmiah Biologi 10(1), 361-382.
Armanzah, R. S., & Hedrawati, T. Y., 2016. "Pengaruh waktu maserasi zat antosianin sebagai pewarna alami dari ubi jalar ungu (Ipomoea batatas L. Poir). Seminar Nasional Sains dan Teknologi, 1-10 November, Indonesia
Bae, I. Y., An, J. S., Oh, I. K., & Lee, H. G., 2017. "Optimized preparation of anthocyanin-rich extract from black rice and its effects on in vitro digestibility." Food Science and Biotechnology 26(5), 1415–1422.
Castañeda-Ovando, A., Pacheco-Hernández, M. de L., Páez-Hernández, M. E., Rodríguez, J. A., & Galán-Vidal, C. A., 2009. "Chemical studies of anthocyanins: A review." Food Chemistry 113(4), 859–871.
Charlton, N. C., Mastyugin, M., Török, B., & Török, M., 2023. "Structural features of small molecule antioxidants and strategic modifications to improve potential bioactivity." Molecules 28(3), 1057.
Choiriyah, N. A., 2017. "Ekstraksi senyawa antosianin dan fenolik rosella ungu dengan variasi pelarut." Darussalam Nutrition Journal 1(1), 16-21.
Damayanti, A., Megawati, M., Mulyani, N. K. C., & Alvionita, E. A., 2020. "Pengaruh perbedaan pelarut asam pada ekstraksi antosianin bunga dadap merah (Erythrina Crista-Galli) dengan metode microwave assisted extraction." Journal of Chemical Process Engineering 5(1), 33–39.
Demir, T., Gunesli, Z., Sonmez, F., Bilen, C., Yavuz, E., & Gencer, N., 2015. "Inhibition of carbonic anhydrase I and II with total anthocyanins extracted from sweet cherry cultivars." Environmental Engineering and Management Journal, 14(4), 935–941.
Djaeni, M., Ariani, N., Hidayat, R., & Utari, F. D., 2017. "Ultrasonic aided anthocyanin extraction of Hibiscus sabdariffa L. flower petal: Antioxidant activity. Jurnal Aplikasi Teknologi Pangan, 6(3), 148-151.
Fathurahmi, S., If’all, & Spetriani., 2022. "Dye extraction of red dragon fruit skin." Jurnal Pengolahan Pangan, 7(2), 75–79.
Fatonah, N., Idiawati, N., & Harlia., 2016. "Uji stabilitas zat warna ekstrak buah senggani (Melastoma malabathricum L.)." Jurnal Kimia Khatulistiwa, 5(1), 29–35.
Fitriani, A. 2017. Ekstraksi antosianin dari ubi jalar ungu (Ipomoea batatas L.) sebagai bahan pewarna minuman skripsi jurusan teknologi pengolahan hasil perikanan. Thesis. Politeknik Pertanian Negeri Pangkep.
Giusti, M. M., & Wrolstad, R. E. 2001. "Characterization and measurement of anthocyanins by UV-visible spectroscopy." Current Protocols in Food Analytical Chemistry, 1(F1-2). https://doi.org/10.1016/j.foodchem.2019.125373
Handaratri, A., & Limantara, L. 2016. "Uji antioksidan antosianin buah murbei dengan ekstraksi sonikasi dan maserasi." Jurnal Siantek 13(2), 94-98.
Handaratri, A., & Yuniati, Y., 2019. "Kajian ekstraksi antosianin dari buah murbei dengan metode sonikasi dan microwave." Reka Buana : Jurnal Ilmiah Teknik Sipil Dan Teknik Kimia, 4(1), 63–67.
Herfayati, P., Pandia, S., & Nasution, H. 2020. "Karakteristik antosianin dari kulit buah nipah (Nypa frutican) sebagai pewarna alami dengan metode soxhletasi." Jurnal Teknik Kimia USU, 09(2), 80–86.
Husna, A., Lubis, Y. M., & Erika, C., 2022. "Ekstraksi pewarna alami dari bunga telang (Clitoria ternatea L.) dengan variasi jenis pelarut dan lama ekstraksi." Jurnal Ilmiah Mahasiswa Pertanian 7(2), 410–419.
Khoo, H. E., Azlan, A., Tang, S. T., & Lim, S. M., 2017. "Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits." Food and Nutrition Research, 61(1), 0–21.
Kostka, T., Ostberg-pottho, J. J., Briviba, K., Matsugo, S., Winterhalter, P., & Esatbeyoglu, T., 2020. "Anthocyanin and copigment fractions — free radical scavenging activity and influence on cellular oxidative stress." Foods, 9(11), 1617.
Kristiana, H. D., Ariviani, S., & Khasanah, L. U. 2012. "Ekstraksi pigmen antosianin buah senggani (Melastoma malabathricum Auct. Non Linn) dengan variasi jenis pelarut." Jurnal Teknosains Pangan 1(1), 105–109.
Kuspradini, H., Rosiarto, A. M., Putri, A. S., & Kusuma, I. W., 2016. "Antioxidant and toxicity properties of anthocyanin extracted from red flower of four tropical shrubs." Nusantara Bioscience, 8(2), 135–140.
Laleh, G. H., Frydoonfar, H., Heidary, R., Jameei, R., & Zare, S., 2006. "The effect of light, temperature, pH and species on stability of anthocyanin pigments in four Berberis species." Pakistan Journal of Nutrition 5(1), 90–92.
Lidya, S., Chairina, S., & Fatimah. 2014. "Ekstraksi pigmen antosianin dari kulit buah naga merah (Hylocereus polyrhizus)." Jurnal Teknik Kimia USU 3(2), 25–29.
Małgorzata B.K., 2017. "Natural flavonoids: classification, potential role, and application of flavonoid analogues." Eur. J. Biol. Res., 7(2), 108–123.
Mazza, G., 2007. "Anthocyanins and heart health." Annali Dell’Istituto Superiore Di Sanita, 43(4), 369–374.
Mori, K., Goto-Yamamoto, N., Kitayama, M., & Hashizume, K., 2007. "Loss of anthocyanins in red-wine grape under high temperature." Journal of Experimental Botany 58(8), 1935–1945.
Pasaribu, S. F., Wiboworini, B., & Kartikasari, L. R., 2021. "Analisis antosianin dan flavonoid ekstrak kecambah beras hitam." Jurnal Dunia Gizi 4(1), 08–14.
Pedro, A. C., Granato, D., & Rosso, N. D., 2016. "Extraction of anthocyanins and polyphenols from black rice (Oryza sativa L.) by modeling and assessing their reversibility and stability." Food Chemistry 191, 12–20.
Pojer, E., Mattivi, F., Johnson, D., & Stockley, C. S., 2013. "The case for anthocyanin consumption to promote human health: A review." Compr. Rev. Food Sci. Food Saf. 12(5), 483–508.
Priyadarshi, R., Ezati, P., & Rhim, J. W., 2021. "Recent advances in intelligent food packaging applications using natural food colorants." ACS Food Sci. Technol. 1(2), 124–138.
Purwaniati, Arif, A. R., & Yuliantini, A., 2020. "Analysis of total anthocyanin content in telang flowers preparations (Clitoria ternatea) with pH differential method using visible spectrophotometry. Jurnal Farmagazine 7(1), 18-23.
Rita, F., Choirun, F.N., 2015. Ekstraksi "Antosianin limbah kulit manggis metode microwave assisted extraction (lama ekstraksi dan rasio bahan : pelarut)." Jurnal Pangan dan Agroindustri 3(2), 362–373.
Sangadji, I., Rijal, M., & Kusuma, Y. A., 2017. "Kandungan antosianin di dalam mahkota bunga beberapa tanaman hias." BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan, 6(2), 118–128.
Sari, N. H. K., 2020. Analisis Kadar Antosianin Total Hasil Ekstraksi Buah Bit (Beta vulgaris) dengan Metode pH Diferensial. Thesis. Universitas dr. Soebandi Jember.
Shiddiqi, Q. Y. A., & Karisma, A. D., 2021. "Ekstraksi senyawa antosianin dari kulit buah naga merah (Hylocereus Polyrhizus) menggunakan metode microwave assisted hydrodistillation (MAHD)." Jurnal Chemurgy, 5(1), 30–37.
Sholihah, A., Aini, N., & Dwiyanti, H., 2021. "Optimasi ekstraksi antosianin pada beras hitam sirampog menggunakan metode ultrasound extract assist (UAE)." Jurnal Aplikasi Teknologi Pangan 10(3), 71–76.
Sholihah, M., Ahmad, U., & Budiastra, I. W., 2016. Ultrasonic-Assisted Extraction Antioksidan Dari Kulit Manggis. Thesis. Bogor Agricultural University (IPB).
Siahaan, L. O., Hutapea, E. R. F., & Tambun, R., 2014. "Ekstraksi pigmen antosianin dari kulit rambutan (Nephelium lappaceum) dengan pelarut etanol." Jurnal Teknik Kimia USU, 3(3), 32–38.
Silva, S., Costa, E. M., Calhau, C., Morais, R. M., & Pintado, M. E., 2017. "Anthocyanin extraction from plant tissues: A review." Crit. Rev. Food Sci. Nutr. 57(14), 3072–3083.
Sumarlan, S. H., Susilo, B., Mustofa, A., & Mu’nim, M. (2018). Ekstraksi senyawa antioksidan dari buah strawberry (Fragaria X Ananassa) dengan menggunakan metode microwave assisted extraction (kajian waktu ekstraksi dan rasio bahan dengan pelarut)." Jurnal Keteknikan Pertanian Tropis dan Biosistem 6(1), 40–51.
Tazar, N., Violalita, F., & Harni, M., 2018. "Pengaruh metoda ekstraksi terhadap karakteristik ekstrak pekat pigmen antosianin dari buah senduduk (Melastoma malabathricum L.) serta kajian aktivitas antioksidannya." Lumbung 17(1), 10–17.
Tena, N., & Asuero, A. G., 2022. "Up-to-date analysis of the extraction methods for anthocyanins: principles of the techniques, optimization, technical progress, and industrial application. Antioxidants 11(2), 286.
Ulya, D., Husna, N. El, & Novita, M., 2018. "Ekstraksi antosianin limbah terung belanda (Solanum betaceaum Cav.) menggunakan metode microwave assisted extraction." Jurnal Ilmiah Mahasiswa Pertanian, 3(1), 409–414.
Unawahi, S., Widyasanti, A., & Rahimah, S., 2022. "Ekstraksi antosianin bunga telang (Clitoria ternatea Linn) dengan metode ultrasonik menggunakan pelarut aquades dan asam asetat." Jurnal Keteknikan Pertanian Tropis dan Biosistem, 10(1), 1–9.
Utami, Y. P., Umar, A. H., & Ernawati., 2016. "Analysis of total anthocyanin content on ethanol extract of purple sweet potato (Ipomoea batatas L.) and purple yam (Dioscoreaalata L.) with differential pH method." Journal of Pharmaceutical and Medicinal Sciences, 1(2), 44–47.
Widyapuri, D., Purbowati, I. S. M., & Wibowo, C., 2022. "Pengaruh waktu ekstraksi menggunakan ultrasonic assisted extraction terhadap antosianin jantung pisang (Musa spp)." Agrointek : Jurnal Teknologi Industri Pertanian, 16(2), 242–251.
Widyastutik, Y., Hardani, P. T., & Sari, D. P., 2022. "Optimasi perbandingan pelarut dan lama maserasi terhadap kadar total antosianin ekstrak jantung pisang (Musa acuminata x Musa balbisiana)." Pharmacon: Jurnal Farmasi Indonesia 19(2), 167–175.
Widyowati, R., & Agil, M., 2018. "Chemical constituents and bioactivities of several Indonesian plants typically used in jamu." Chem. Pharm. Bull. 66(5), 506–518.
Yuniati, Y., Qodim, A., & Qadariyah, L., 2022. "Ekstraksi zat warna kelopak bunga telang (Clitoria ternatea L.) dengan metode ekstraksi berbantuan ultrasonik dan aplikasinya untuk minuman." Int. J. Chem. Process Eng. Res. 7 (2), 79-84.
Zhang, Q. W., Lin, L. G., & Ye, W. C., 2018. "Techniques for extraction and isolation of natural products: A comprehensive review." Chinese Medicine 13(1), 1–26.
Zulfajri, M., & Muttakin., 2017. Metode ekstraksi antosianin dari kulit buah Syzygium cumini (L) skeels sebagai indikator alami asam basa. Seminar Nasional II USM 2017: Eksplorasi Kekayaan Maritim Aceh di Era Globalisasi dalam Mewujudkan Indonesia debagai Poros Maritim Dunia, 1 Oktober 2017, 547–553.
Zuri, R. 2022. "Optimasi pelarut ekstraksi antosianin dari ubi jalar ungu (Ipomoea batatas L. Poir) untuk deteksi boraks dalam makanan." Atmosphere, 3(1), 8–13.
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