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VIABILITY OF BHK-21 FIBROBLAST CELLS TOWARD ACRYLIC DENTURE BASES AFTER REINFORCED BY NATURAL FIBERS
https://doi.org/10.22146/teknosains.90278 Endang Prawesthi(1*), Marzia Magdalena Tetelepta(2), Heldayani Heldayani(3), Ria Fajarwati Kastian(4), Endah Puji Septisetyani(5)
(1) Department of Dental Technology, Politeknik Kesehatan Kemenkes Jakarta II
(2) Department of Dental Technology, Politeknik Kesehatan Kemenkes Jakarta II
(3) Department of Dental Technology, Politeknik Kesehatan Kemenkes Jakarta II
(4) Research Center for Genetic Engineering, National Research and Innovation Agency, KST (BRIN)
(5) Research Center for Genetic Engineering, National Research and Innovation Agency, KST (BRIN)
(*) Corresponding Author
Abstract
The use of acrylic denture bases in the oral cavity requires biocompatibility. This study investigated the viability of BHK-21 fibroblast cells after treatment with an acrylic denture base modified using natural fibers. Ramie and banana stem fibers were used as cost-effective alternatives to synthetic fibers. The study involved 42 acrylic resin specimens (10 mm diameter, 2 mm thickness) divided into groups: resin without fibers, 0.5%, 1.5%, and 2.5% ramie fibers, and 0.5%, 1.5%, and 2.5% banana stem fibers. The resin was incubated with cell culture media at 37°C for 7 days. Cytotoxicity testing using the MTT method revealed that all treatment groups had cell viability exceeding 70%, meeting ISO 10993-5 standards. No significant differences in cell viability were observed between the treatment groups and the control (media without specimens). Additionally, adding 0.5%, 1.5%, and 2.5% ramie fibers did not affect BHK-21 cell viability compared to the resin-only control, while adding banana stem fibers increased cell viability compared to the control (P = 0.035; P = 0.021; and P = 0.011). In conclusion, increasing the concentration of natural fibers in acrylic denture bases did not negatively impact fibroblast cell growth.
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DOI: https://doi.org/10.22146/teknosains.90278
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