Analisis sitogenetik sel epitel mukosa bukal pekerja stasiun pengisi bahan bakar umum di kota Yogyakarta

https://doi.org/10.22146/teknosains.43852

Regina TC. Tandelilin(1), Elastria Widita(2*), Rini Maya Puspita(3), Tang Sze Mun(4)

(1) Fakultas Kedokteran Gigi, Universitas Gadjah Mada
(2) Fakultas Kedokteran Gigi, Universitas Gadjah Mada
(3) Fakultas Kedokteran Gigi, Universitas Gadjah Mada
(4) Mahasiswa Program Studi Profesi Program Studi Pendidikan Dokter Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Workers employed in petroleum station have a high-risk exposure to a wide range of toxic compounds with known mutagenic and carcinogenic potential. Cytogenetic damage might have happened if they continuously exposed to petroleum derivatives. This study aimed to analyse the cytogenetic damage in exfoliated buccal cells among petroleum station workers in Yogyakarta City. This cross-sectional study was carried out on 30 petrol station workers who are working at a different petrol station in Yogyakarta and the control group consisted of 30 healthy subjects. Examination for all subjects included frequencies of nuclear abnormalities, including pycnosis, karyorrhexis, and karyolysis. Cytological preparations were stained according to papanicolaou reaction and analyzed under light microscope for making a score for degenerative nuclear alterations (pycnosis, karyolysis and karyorrhexis). Analysis of buccal cells revealed that frequencies of pycnosis and karyorrhexis in petrol station workers were significantly higher than the control group (P<0.05). Conversely, there was no significant difference in karyolisis among groups. These findings indicate that the petrol station workers are under the risk of significant cytogenetic damage, particularly pycnosis and karyorrhexis.

 


Keywords


buccal cells; pycnosis; karyolysis; karyorrhexis; petrol station workers

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References

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field-separator'>Alwakeel, S. S. (2017). Microbiological and molecular identification of bacterial species isolated from nasal and oropharyngeal mucosa of fuel workers in Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences, 24(6): 1281-1287.

Baan, R., Grosse, Y., Straif, K., Secretan, B., El Ghissassi, F., Bouvard, V., Benbrahim-Tallaa, L., Guha, N., Freeman, C., Galichet, L. dan Cogliano, V. (2009). A review of human carcinogens. Part F: Chemical agents and related occupations. The Lancet Oncology, 10(12): 1143-1144.

Bavle, R. M. (2014). Mitosis at a glance. Journal of Oral and Maxillofacial Pathology, 18(Suppl 1): S2-S5.

Berkovitz, B., Moxham, B., Linden, R. dan Sloan, A. (2010). Master Dentistry Volume 3 Oral Biology: Elsevier.

Borthakur, G., Butryee, C., Stacewicz-Sapuntzakis, M. dan Bowen, P. E. (2008). Exfoliated Buccal Mucosa Cells as a Source of DNA to Study Oxidative Stress. Cancer Epidemiology Biomarkers & Prevention, 17(1): 212-219.

Brosselin, P., Rudant, J., Orsi, L., Leverger, G., Baruchel, A., Bertrand, Y., Nelken, B., Robert, A., Michel, G., Margueritte, G., Perel, Y., Mechinaud, F., Bordigoni, P., Hemon, D. dan Clavel, J. (2009). Acute childhood leukaemia and residence next to petrol stations and automotive repair garages: the ESCALE study (SFCE). Occupational and Environmental Medicine, 66(9): 598-606.

Chaiklieng, S., Suggaravetsiri, P. dan Autrup, H. (2019). Risk Assessment on Benzene Exposure among Gasoline Station Workers. International Journal of Environmental Research and Public Health, 16(14): 2545.

Dash, A., Singh, S. dan Tolman, J. (2013). Pharmaceutics: Basic Principles and Application to Pharmacy Practice: Elsevier.

Demirhan, O. dan Tastemir, D. (2008). Cytogenetic effects of ethanol on chronic alcohol users. Alcohol and Alcoholism, 43(2): 127-36.

Edokpolo, B., Yu, Q. J. dan Connell, D. (2015). Health risk characterization for exposure to benzene in service stations and petroleum refineries environments using human adverse response data. Toxicol Rep, 2: 917-927.

Elesawy, B. H., Abd El Hafez, A., Shawky Mohamed, A. dan Mansour A. Salih, M. (2016). Application of Bethesda terminology to categorize buccal epithelial smears among petroleum station workers in Taif city, KSA. 2016, 5(3): 5.

Holland, N., Bolognesi, C., Kirsch-Volders, M., Bonassi, S., Zeiger, E., Knasmueller, S. dan Fenech, M. (2008). The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutation Research, 659(1-2): 93-108.

Joshi, M. S., Verma, Y., Gautam, A. K., Parmar, G., Lakkad, B. C. dan Kumar, S. (2011). Cytogenetic alterations in buccal mucosa cells of chewers of areca nut and tobacco. Arch Oral Biol, 56(1): 63-7.

Keretetse, G. S., Laubscher, P. J., Du Plessis, J. L., Pretorius, P. J., Van Der Westhuizen, F. H., Van Deventer, E., Van Dyk, E., Eloff, F. C., Van Aarde, M. N. dan Du Plessis, L. H. (2008). DNA damage and repair detected by the comet assay in lymphocytes of african petrol attendants: a pilot study. Annals of Occupational Hygiene, 52(7): 653-62.

Kroemer, G., El-Deiry, W. S., Golstein, P., Peter, M. E., Vaux, D., Vandenabeele, P., Zhivotovsky, B., Blagosklonny, M. V., Malorni, W., Knight, R. A., Piacentini, M., Nagata, S. dan Melino, G. (2005). Classification of cell death: recommendations of the Nomenclature Committee on Cell Death. Cell Death and Differentiation, 12 Suppl 2: 1463-7.

Lima, C. F., Oliveira, L. U., Cabral, L. A., Brandao, A. A., Salgado, M. A. dan Almeida, J. D. (2010). Cytogenetic damage of oral mucosa by consumption of alcohol, tobacco and illicit drugs. Journal of Oral Pathology and Medicine, 39(6): 441-6.

Martins, R. A., Gomes, G. A., Aguiar, O., Jr. dan Ribeiro, D. A. (2009). Biomonitoring of oral epithelial cells in petrol station attendants: comparison between buccal mucosa and lateral border of the tongue. Environment International, 35(7): 1062-5.

Metgud, R., Khajuria, N., Patel, S. dan Lerra, S. (2015). Nuclear anomalies in exfoliated buccal epithelial cells of petrol station attendants in Udaipur, Rajasthan. Journal of Cancer Research and Therapeutics, 11(4): 868-73.

Misra, A., Rai, S. dan Misra, D. (2016). Functional role of apoptosis in oral diseases: An update. Journal of oral and maxillofacial pathology : JOMFP, 20(3): 491-496.

Moro, A. M., Brucker, N., Charão, M., Bulcão, R., Freitas, F., Baierle, M., Nascimento, S., Valentini, J., Cassini, C., Salvador, M., Linden, R., Thiesen, F., Buffon, A., Moresco, R. dan Garcia, S. C. (2012). Evaluation of genotoxicity and oxidative damage in painters exposed to low levels of toluene. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 746(1): 42-48.

Motgi, A., Chavan, M., Diwan, N., Chowdhery, A., Channe, P. dan Shete, M. (2014). Assessment of cytogenic damage in the form of micronuclei in oral epithelial cells in patients using smokeless and smoked form of tobacco and non-tobacco users and its relevance for oral cancer. Journal of Cancer Research and Therapeutics, 10(1): 165-170.

Nikitakis, N. G., Sauk, J. J. dan Papanicolaou, S. I. (2004). The role of apoptosis in oral disease: Mechanisms; aberrations in neoplastic, autoimmune, infectious, hematologic, and developmental diseases; and therapeutic opportunities. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 97(4): 476-490.

Palaskar, S. dan Jindal, C. (2010). Evaluation Of Micronuclei Using Papanicolaou And May Grunwald Giemsa Stain In Individuals With Different Tobacco Habits – A Comparative Study. Journal of Clinical and Diagnostic Research, 4(6): 3607-3613.

Paul, S., Chakraborty, A., Deb, B. dan Roy, P. (2016). Nuclear abnormalities in exfoliated buccal epithelial cells of petrol pump attendants of Southern Assam, India. Journal of Global Biosciences, 5(1): 3491-3499.

Rivera, C. dan Núñez-De-Mendoza, C. (2013). Exfoliative cytology of oral epithelial cells from patients with type 2 diabetes: cytomorphometric analysis. International Journal of Clinical and Experimental Medicine, 6(8): 667-676.

Rocha, L. P., Cezar-Vaz, M. R., Almeida, M. C. V. D., Bonow, C. A., Silva, M. S. D. dan Costa, V. Z. D. (2014). Use of personal protective equipment by gas stations workers: a nursing contribution. Texto & Contexto - Enfermagem, 23: 193-202.

Squier, C. dan Brogden, K. (2010). Human Oral Mucosa: Development, Structure and Function: Wiley-Blackwell.

Steinmaus, C. dan Smith, M. T. (2017). Steinmaus and Smith Respond to "Proximity to Gasoline Stations and Childhood Leukemia". Am J Epidemiol, 185(1): 5-7.

Tolbert, P. E., Shy, C. M. dan Allen, J. W. (1991). Micronuclei and other nuclear anomalies in buccal smears: a field test in snuff users. Am J Epidemiol, 134(8): 840-50.

Tolbert, P. E., Shy, C. M. dan Allen, J. W. (1992). Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutation Research/Environmental Mutagenesis and Related Subjects, 271(1): 69-77.

Torres-Bugarín, O., Zavala-Cerna, M. G., Nava, A., Flores-García, A. dan Ramos-Ibarra, M. L. (2014). Potential uses, limitations, and basic procedures of micronuclei and nuclear abnormalities in buccal cells. Disease Markers, 2014: 1-13.

Tucker, J. D. dan Moore, D. H., 2nd (1996). The importance of age and smoking in evaluating adverse cytogenetic effects of exposure to environmental agents. Environmental Health Perspectives, 104 Suppl 3: 489-92.

Tunsaringkarn, T., Prueksasit, T., Kitwattanavong, M., Siriwong, W., Sematong, S., Zapuang, K. dan Rungsiyothin, A. (2012). Cancer risk analysis of benzene, formaldehyde and acetaldehyde on gasoline station workers. journal of Environmental Engineering and Ecological Science, 1(1).

Zhang, L., Eastmond, D. A. dan Smith, M. T. (2002). The nature of chromosomal aberrations detected in humans exposed to benzene. Critical Reviews in Toxicology, 32(1): 1-42.

Zhang, Y. (2018). Cell toxicity mechanism and biomarker. Clinical and translational medicine, 7(1): 34-34.

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DOI: https://doi.org/10.22146/teknosains.43852

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