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POTENSI TANAMAN DI TAMAN NASIONAL PULAU JAWA SEBAGAI SUMBER BAKTERI PENGHASIL ANTIBIOTIK
https://doi.org/10.22146/teknosains.26853 Sri Yuwantiningsih(1*), Sebastian Margino(2), Subagus Wahyuono(3)
(1) Akademi pertanian Yogyakarta
(2) Program Studi Bioteknologi Sekolah PascasarjanaUniversitas Gadjah Mada
(3) Program Studi Bioteknologi Sekolah PascasarjanaUniversitas Gadjah Mada
(*) Corresponding Author
Abstract
Bacterial endophytes in trees are potential source to obtain new antibiotic producers that have not been widely studied until now. This study aims to obtain bacterial endophytes isolates in the tissue of twig trees in National Park Banten, Indonesia Germplasm Garden (The Bogor Botanical Gardens), Cilacap Nusakambangan Island, Kaliurang National Park, Meru Betiri National Park Jember, and Baluran National Park, Situbondo. This selection was based on the growth ability in liquid nutrient, GY (glucose and yeast-extract), and antibiotic-3, and the ability to obstruct indicator microbes, such as Bacillus subtilis, Candida albicans, and Fusarium oxysporum. The selection result is obtained eight isolates that have inhibitory effect more than 4, such as BIN-1, KLP-1, OOH-1, STG-1, CMB-2, STL-1, MTG-1, and PPH-1. Based on chromatography method on various eluents, it is obtained three superior isolates, i.e OOH-1 ,STG-1, and CMB-2. Thus, this shows that bacterial endophytes in trees constitute potential sources for screening new antibiotic producers.
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• Joko, T. dan Kusumandari, N. 2014. “Deteksi molekuler bakteri penyebab penyakit busuk lunak pada anggrek menggunakan teknik Polymerase Chain Reaction. Pengembangan dan pemanfaatan IPTEKS untuk kedaulatan pangan.” Seminar Nasional Dies Natalis Faperta UGM ke-68. Yogyakarta: UGM.
• Harni, R., Supramana., Sinaga M.S., Giyanti dan Supriyadi. 2010. “Pengaruh Filtrat Bakteri Endofit Terhadap Normalitas Penetasan Telur dan Populasi Nematoda Peluka Akar Pratylen chus brachyurus pada Nilam.” Journal Litr, 16(1):43-47.
• He. R.I., Wang. G.P., Liu. X.H., Zhang, C.L and Lin.F.C. 2009. “Antagonistic bioactivity of an endophytic bacterium isolated from Epimedium brevicornu.” Maxim African Journal of Biotecnology, 8. (2): 191-195.
• Lestiyani A.; Wibowo A.; Subandiyah S. 2014. “Uji Metode Inokulasi Pada Bawang merah dengan Fusarium spp. Pengembangan dan Pemanfaatan IPTEKS untuk Kedaulatan Pangan.” Seminar Nasional Dies Natalis Faperta UGM ke-68. Yogyakarta: UGM.
• Lockhart, SL., Kiederma DJ., dan Pfaller MA. 2009. “The Epidemiology of Fungal Infection.” Diedit oleh Anaissie, EJ, Mc Ginnis MR, Pfaller MA. Journal of Clinical Mycologv. Edisi kedua. Oxford V K Elsevi Sp Inc.
• Margino S., 2008. “Produksi metabolit sekunder (antibiotik) oleh jamur endofit.” Majalah Farmasi Indonesia, 19:86-94
• Meliawati R, Widyaningrum D.N., Djohan A.C., dan Sukiman, H. 2006. “Pengkajian Bakteri Endofit Penghasil Senyawa Bioaktif untuk Proteksi Tanaman.” Jurnal Biodiversitas, 7(3):221-224.
• Mubin, F. 1999. “Kajian Produksi Senyawa Antimikrobia oleh Mikrobia Endofit.” Skripsi. Fakultas Pertanian Universitas Gadjah Mada.
• Muhammed,M., Fuchs, B., Wu, M.P., Breger. J., Coleman J.J., dan Mylonakis. E. 2012. “The role of mycelium production and a MAPK-mediated imune response in the C. elegans – Fusarium model system.” Journal of Medical Mycology, 50(5) 488-496.
• Nasution A., Usyati, N., dan Santosa. 2014. “Varietas lokal padi sebagai sumberketahanan penyakit blas daun dan blas leher. Pengembangan dan pemanfaatan IPTEKS untuk kedaulatan pangan.” Seminar Nasional Dies Natalis Faperta UGM ke-68. Yogyakarta: UGM.
• Nugroho E., 2009. “Potensi Daya Antibakteri isolat Lactobacillus dari Tempoyak Escherichia coli.” Seminar Hasil Penelitian dan Pengabdian Masyarakat. Uniba.
• Patel., H.A. Khristi., SM, Perikh. K, Rajeram G. 2012. “Isolation and Characterization of Bacterial endophytic from Lycopersicum esculentum plant and their plant promoting characteristics.” Nepal. Journal of Biotechnology, 2 (1):37-52.
• Smitha SL and Philip RK. 2014. “Antibiotik Organic Compoundproduction by a marine fungus Pencillium cirinum S36 through solid state fermentation: optimization by response surface methodology.” International Journal of Research Biomedicine and Biotechnology, 4(1):6-13.
• Snook, M. E., Mitchel, T., Hinton, D. M., dan Bacon, C. W., 2009. Isolation and Characterization of Leu surfaction from the endophytic bacterium B.mojavensis RRC. 101., a Biocontrol for Fusarium verticulodies. Journal of Agriculture and Food Chemical, 57:4287-4292.
• Strobel G., Daisy B., Castillo U., Harper J. 2004. “Natural Product from Microorganism.” Journal of Natural Product, 67:257-269.
• Widiastuti, A. dan Ningtyas, O. H. 2014. “Identifikasi jenis jamur penyebab busuk pada beberapa buah pasca panen di Yogyakarta. Pengembangan dan pemanfaatan IPTEKS untuk kedaulatan pangan.” Seminar Nasional Dies Natalis Fapefta UGM ke-68. Yogyakarta: UGM.
• Zinniel, D. K., Lambrecht; Harris, N. B.; Fang, Z; Kuczmarsk, I. D.; Higley, P; Ishimaru, C; Arunakumari, A; Barletta, R. G.; Vidaver, A. K. 2002. “Isolation and Characterization of Endophytic Colonizi Bacteria from Agronomic Crops and Praire Plant.” Applied Environment Microbiology. 68(5):2198-2108.
DOI: https://doi.org/10.22146/teknosains.26853
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