* Corresponding Author
Antibiofilm activity of Cyanobacteria spirulina as an irrigation solution against Enterococcus faecalis
https://doi.org/10.22146/majkedgiind.78246 Diana Soesilo(1*), Fani Pangabdian(2), Eriza Juniar(3)
(1) Department of Conservative Dentistry, Universitas Hang Tuah, Surabaya, East Java
(2) Department of Conservative Dentistry, Universitas Hang Tuah, Surabaya, East Java
(3) Department of Pediatric Dentistry, Universitas Hang Tuah, Surabaya, East Java
(*) Corresponding Author
Abstract
Sodium hypochlorite (NaOCl) is currently the golden standard for root canal irrigation. NaOCl at a concentration of 5.25% to 6% can eliminate E. faecalis, but this concentration can increase the risk of toxic effects. Cyanobacteria spirulina is known to produce several secondary metabolites that have antimicrobial activity against gram-positive and gram-negative bacteria. The aim of this study was to determine the antibiofilm power of Cyanobacteria spirulina against the biofilms of Enterococcus faecalis at concentrations of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml. This research was a true experiment with a post-test only group design. The object of the research was divided into 6 groups. Group 1 was a negative control group, group 2 was a positive control group with 5% NaOCl, group 3, 4, 5, 6 were treatment groups given Cyanobacteria spirulina solution at a concentration of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml, respectively. Optical density (OD) of bacteria was bound by staining and analyzed by ELISA auto reader with a wave length of 595 nm (OD 595 nm). The results of the LSD test showed that the significance between K+ and P1, P2, P3 (p< 0.005), K+ and P4 (p= 0.129), P1 and P2 (p=0.449), P3 and P4 (p=0.178). Significance of p<0.0005 showed a significant difference between the groups. The data were analyzed using the one-way ANOVA test followed by a double comparison test with the Least Significance Different (LSD) Post Hoc test method. Cyanobacteria spirulina solution at a concentration of 90 mg/ml had the greatest inhibitory effect on the biofilm formation of Enterococcus faecalis.
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DOI: https://doi.org/10.22146/majkedgiind.78246
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