* Corresponding Author
Antimicrobial photodynamic therapy with erythrosine photosensitizer against immune response in chronic periodontitis model
https://doi.org/10.22146/majkedgiind.77084 Mutia Rochmawati(1*), Maulidina Raihan Kusuma(2), Faiz Maziyyah(3), Cantika Nadrotan Naim(4), Christiana Cahyani Prihastuti(5), Rinawati Satrio(6), Anindita Laksitasari(7), Dwi Nur Indah Sari(8), Meylida Ichsyani(9)
(1) Study Program of Dental Medicine, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Central Java
(2) Study Program of Dental Medicine, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Central Java
(3) Study Program of Dental Medicine, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Central Java
(4) Study Program of Dental Medicine, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Central Java
(5) Study Program of Dental Medicine, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Central Java
(6) Study Program of Dental Medicine, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Central Java
(7) Study Program of Dental Medicine, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Central Java
(8) Study Program of Dental Medicine, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Central Java
(9) Study Program of Dental Medicine, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Central Java
(*) Corresponding Author
Abstract
Chronic periodontitis is a progressive inflammatory disease of the supporting tissues of the teeth caused by dental plaque bacteria with a clinical sign of periodontal pockets. A Gram-negative bacterium that can trigger this inflammatory disease is Porphyromonas gingivalis. Antimicrobial photodynamic therapy with blue LED light irradiation and photosensitizer erythrosine can reduce the survival rate of P. gingivalis. This study aimed to determine the effects of antimicrobial photodynamic therapy (APDT) exposure with blue LED light irradiation and PS erythrosine on the number of macrophages, lymphocytes, and gingival fibroblasts in gingival tissue of Sprague Dawley rats as chronic periodontitis models. This study used a posttest-only control group design to examine 27 Sprague Dawley rats which were divided into P group (healthy rats), N group (untreated chronic periodontitis rats), and PDT groups (chronic periodontitis model given 1 mg/ml PS erythrosine and irradiated with blue LED light for 60 seconds). Cell observation of histologic preparations of rat gingival tissue with hematoxylin-eosin (H&E) staining was carried out on the 1st, 3rd, and 5th days. Histological preparations of gingival tissue with H&E staining was carried out on the 1st, 3rd, and 5th days. Statistical analysis used a one-way ANOVA and the Kruskal-Wallis test, continued with LSD and the Mann-Whitney post-hoc tests. Results showed significant difference in the mean of macrophages in the PDT group compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). The mean lymphocyte in the PDT group was significantly different from the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05), and significantly lower than that in the healthy group (p < 0.05) but only on the 3rd and 5th days. The mean fibroblast in the PDT group was significantly different compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). In conclusion, there were significant differences in the number of macrophages, lymphocytes, and fibroblasts in a chronic periodontitis rat model after treatment with APDT exposure with blue LED and erythrosine photosensitizer.
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1. Wulandari P, Widkaja D, Nasution AN, Syahputra A, Gabrina G. Association between age, gender and education level with the severity of periodontitis in pre-elderly and elderly patients. Dental Journal (Majalah Kedokteran Gigi). 2022; 55(1): 16–20.
doi: 10.20473/j.djmkg.v55.i1.p16–20
2. Cekici A, Kantarci A, Hasturk H, Van Dyke TE. Inflammatory and immune pathways in
the pathogenesis of periodontal disease. Periodontol 2000. 2014; 64(1): 57-80.
doi: 10.1111/prd.12002
3. Newman MG, Takei H, Klokkevold PR, Carranza FA. Newman and Carranza's clinical
periodontology. Elsevier Health Sciences; 2018.
4. Mombelli A. Microbial colonization of the periodontal pocket and its significance for
periodontal therapy. Periodontology 2000. 2018; 76(1): 85-96. doi: 10.1111/prd.12147
5. Vos T, Abajobir AA, Abate KH, Abbafati C, Abbas KM, Abd-Allah F, Abdulkader RS,
Abdulle AM, Abebo TA, Abera SF, Aboyans V. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries,
1990–2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet.
2017; 390(10100): 1211-1259. doi: 10.1016/S0140-6736(17)32154-2
6. Liu Y, Qin R, Zaat SA, Breukink E, Heger M. Antibacterial photodynamic therapy: overview
of a promising approach to fight antibioticresistant bacterial infections. Journal of
Clinical and Translational Research. 2015; 1(3): 140. doi:10.18053/jctres.201503.002
7. Rossi F, Magni G, Tatini F, Banchelli M, Cherchi F, Rossi M, Coppi E, Pugliese AM, Rossi degl’Innocenti D, Alfieri D, Pavone FS. Photobiomodulation of human fibroblasts and keratinocytes with blue light: implications in wound healing. Biomedicines. 2021; 9(1): 41.
doi:10.3390/biomedicines9010041
8. Park JH, Kim YG, Um HS, Lee SY, Lee JK, Chang BS. Antimicrobial effect of toothbrush
with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study.
Journal of Dental Rehabilitation and Applied Science. 2019; 35(3): 160-169.
doi: 10.14368/jdras.2019.35.3.160
9. Mahdi Z, Habibollah G, Mahbobeh NN, Mina ZJ, Majid Z, Nooshin A. Lethal effect of blue light-activated hydrogen peroxide, curcumin and erythrosine as potential oral photosensitizers on the viability of Porphyromonas gingivalis and Fusobacterium nucleatum. Laser therapy. 2015; 24(2): 103-111. doi: 10.5978/islsm.15-OR-09.
10. de Oliveira PG, Silveira e Souza AM, Novaes AB, Taba M, Messora MR, Palioto DB, Grisi
MF, Tedesco AC, de Souza SL. Adjunctive effect of antimicrobial photodynamic therapy in induced periodontal disease. Animal study with histomorphometrical, immunohistochemical, and cytokine evaluation. Lasers in medical science. 2016; 31(7): 1275-1283. doi: 10.1007/s10103-016-1960-5
11. Putri CF, Bachtiar EW. Porphyromonas gingivalis dan patogenesis disfungsi kognitif:
analisis peran sitokin neuroinflamasi. Cakradonya Dental Journal. 2020; 12(1): 15-23.
12. Fracalossi C, Nagata JY, Pellosi DS, Terada RSS, Hioka N, Baesso ML, Sato F, Rosalen PL, Caetano W, Fujimaki M. Singlet oxygen production by combining erythrosine and halogen light for photodynamic inactivation of Streptococcus mutans. Photodiagnosis Photodyn Ther. 2016; 15: 127–132. doi: 10.1016/j.pdpdt.2016.06.011
13. Indrawati R, Lolita AM, Limantara L. Terapi fotodinamik antimikroba: prospek baru dalam
penanganan pangan?. Jurnal Ilmiah Berkala Sains dan Terapan Kimia. 2021; 15(1): 74-90.
14. Habiboallah G, Mahdi Z, Mahbobeh NN, Mina ZJ, Sina F, Majid Z. Bactericidal effect of
visible light in the presence of erythrosine on Porphyromonas gingivalis and Fusobacterium
nucleatum compared with diode laser, an in vitro study. Laser Therapy. 2014; 23(4): 263-
271. doi: 10.5978/islsm.14-OR-20
15. Könönen E, Gursoy M, Gursoy UK. Periodontitis: a multifaceted disease of tooth-supporting tissues. Journal of clinical medicine. 2019; 8(8): 1135. doi: 10.3390/jcm8081135
16. Astuti SD, Utomo IB, Setiawatie EM, Khasanah M, Purnobasuki H, Arifianto D, Alamsyah
KA. Combination effect of laser diode for photodynamic therapy with doxycycline on a wistar rat model of periodontitis. BMC oral health. 2021; 21(1): 1-5. doi:10.1186/s12903-021-01435-0
17. Abbas AK, Lichtman AH, Pillai S. Basic immunology: functions and disorders of the
immune system. Singapore: Elsevier. 2020 (6): 276–257.
18. biScheres N, Crielaard W. Gingival fibroblast responsiveness is differentially affected by
Porphyromonas gingivalis: implications for the pathogenesis of periodontitis. Molecular
oral microbiology. 2013; 28(3): 204-218. doi:10.1111/omi.12016
19. Landén NX, Li D, Ståhle M. Transition from inflammation to proliferation: a critical step during wound healing. Cellular and Molecular Life Sciences. 2016; 73(20): 3861-3885. doi:10.1007/s00018-016-2268-0
20. Cheng R, Choudhury D, Liu C, Billet S, Hu T, Bhowmick NA. Gingival fibroblasts resist
apoptosis in response to oxidative stress in a model of periodontal diseases. Cell Death Discovery. 2015; 1(1): 1-8. doi:10.1038/cddiscovery.2015.46
21. Chui C, Aoki A, Takeuchi Y, Sasaki Y, Hiratsuka K, Abiko Y, lzumi Y. Antimicrobial
effect of photodynamic therapy using highpower blue light-emitting diode and reddye
agent on Porphyromonas gingivalis. J Periodontal Res. 2013; 48(6): 696–705.
doi: 10.1111/jre.12055
22. Gonçalves ML, Santos EM, Renno AC, Horliana AC, de Almeida Cruz M, Parisi JR, Prates RA, Leal-Rossi A, Fernandes KP, Mesquita-Ferrari RA, Bussadori SK. Erythrosine as a photosensitizer for antimicrobial photodynamic therapy with blue light-emitting diodes-An in vitro study.
Photodiagnosis Photodyn Ther. 2021; 35: 102445. doi: 10.1016/j.pdpdt.2021.102445
23. Anantawan J, Damrongrungruang T, Teerakapong A. Effects of Erythrosine With/out Nano-TiO2 Dioxide-Mediated Photodynamic Therapy On HGF-1 and HOK Cells By PrestoBlue® Viability Assay. InRangsit Graduate Research Conference: RGRC. 2018; 13: 3100-3111.
24. Chen S, Tang L, Xu M, Chen T, Zhao S, Liu M, Liu S. Light‐emitting‐diode‐based antimicrobial photodynamic therapies in the treatment of periodontitis. Photodermatol Photoimmunol Photomed. 2022; 38(4): 311-321. doi: 10.1111/phpp.12759
25. Longo M, Garcia VG, Ervolino E, Alves ML, Duque C, Wainwright M, Theodoro LH. Multiple aPDT sessions on periodontitis in rats treated with chemotherapy: histomorphometrical, immunohistochemical, immunological and microbiological analyses. Photodiagnosis Photodyn Ther. 2019; 25: 92-102. doi: 10.1016/j.pdpdt.2018.11.014
DOI: https://doi.org/10.22146/majkedgiind.77084
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