* Corresponding Author
Potency of stored DNA derived from buccal mucosal epithelial cells for genotype analysis of interleukin 4 -590 C/T
https://doi.org/10.22146/majkedgiind.37361 Rezmelia Sari(1*), Alya Nur Fadhilah(2), Prayitno Prayitno(3)
(1) Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Dentistry Study Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author
Abstract
The genotype variation of the interleukin 4 (IL-4) gene on spot -590 C/T is known to correlate with IL-4 protein expression in periodontitis, but the results found in the population are inconsistent. Sequencial genotype analysis of multiple genes often used stored DNA samples obtained from buccal mucosal epithelial cells. This study aims to determine the use of stored DNA samples obtained from buccal mucosal epithelial cells for genotype analysis specifically on -590 C/T IL-4 genes. This is a descriptive analytical study. The samples were 58 DNA derived from the buccal mucosal epithelial cells taken by sterile cotton swab isolated with PrestoTM kit (GeneAid). The DNA has been stored for 2 years at -20 °C. The genotype analysis was conducted using PCR RFLP technique with BSmFI enzyme and the data were presented descriptively. The results show that 51.72% of the stored DNA is still in good condition and 37.9% can be used for genotype analysis. It was concluded that DNA derived from buccal mucosal epithelial cells stored at -20 °C for 2 years can still be used for genotype analysis, but the quality of DNA is affected by storage time.
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1. Lanni M, Bruzzesi G, Pugliese D, Porcellini E, Carbone I, Schiavone A, Licastro F. Variations in inflammatory genes are associated with periodontitis. Immunity and Ageing. 2013; 10(39): 1-8. doi: 10.1186/1742-4933-10-39
2. Mulot C, Stucker I, Clavel J, Beune P, Loriot MA. Collection of human genetic DNA from buccal cells for genetic studies: comparison between cytobrush, mouthwash, and treated card. J Biotechnol. 2005; 2005(3): 291-296. doi: 10.1155/JBB.2005.291
3. Bartova J, Linhartova PB, Podzimek S, Janatova T, Svobodova K, Fassmann A,
Duskova J, Belacek J, Holla LI. The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls. Mediators Inflamm. 2014; 2014: 185757. doi: 10.1155/2014/185757
4. Newman MG, Takei HH, Klokkevold PR. Carranza’s Clinical Periodontology; 2015. 473.
5. Heidari Z. The association between Proinflammatory gene polymorphsms and level of gingival tissue degradation in chronic periodontitis. Gene Cell Tissue. 2014; 1: 2.
6. Psifidi A, Dovas CI, Bramis G, Lazou T, Russel CL, Arsenos G, Banos G. Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples. PLoS One. 2015; 10(1): e0115960. doi: 10.1371/journal.pone.0115960
7. Finkel MA, Giardino AP. Medical Evaluation of Child Sexual Abuse. New Delhi: Sage Publication; 2002. 139.
8. Tanjaya J, Auerkari EI. IL-1β genetic polymorphism in menopause women as periodontal disease risk factor. Journal of Dentistry Indonesia. 2011; 18(1): 1-5. doi: 10.14693/jdi.v18i1.52
9. Kwok P, Chen X. Detection of single nucleotide polymorphisms. Curr Issues Mol Biol. 2003; 5(2): 43-60.
10. Robati M, Ranjbari A, Boroujerdnia M, Chinipardaz Z. Detection of IL-4, IL-6, and IL-12 serum levels in generalized aggresive periodontitis. Iran J Immunol. 2011; 8(3): 170-175.
11. Atanasovska-Stojanovic A, Trajkov D, Nares S, Angelov N, Spiroski M. IL-4 gene polymorphisms and their relation to periodontal disease in Macedonian population. Hum Immunol. 2011; 72(5): 446-450. doi: 10.1016/j.humimm.2011.02.005.
DOI: https://doi.org/10.22146/majkedgiind.37361
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