The effect of papaya leaf extract (Carica papaya L) on healing process of buccal traumatic ulcer in wistar rats

https://doi.org/10.22146/majkedgiind.37026

Afryla Femilian(1*), Dewi Agustina(2), Goeno Subagyo(3)

(1) Department of Oral Medicine, Dental school, Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta, Yogyakarta Master of Clinical Dental Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Indonesians have been using herbal medicines for a long time to cure some illnesses. Carica papaya L is an example of an herb that contains papain enzymes, saponins, lysozymes, lipases, flavonoids, polyphenols and vitamin C. These  ingredients are believed to be beneficial for the wound healing process. The purpose of this study was to determine the effect of topical application of ethanolic extract of Carica papaya L to the healing process of rat mouth ulcer. Subjects were 32 Wistar rats divided into 2 groups (control group and treatment group), each group containing 16 rats. All subjects were made to suffer from ulcers using glacial acetyl acid applied for 40 seconds in the buccal mucosa. Treatment group was treated with papaya leaf ethanolic extract on ulcers twice a day using microbrush, while the ulcer in the control group was not treated with the extract. The ulcerated tissue was biopsied and stained with H&E. Observations were performed on the day 0, 3rd, 7th and 12th on HE slides. Data were observed by looking at three indicators of wound healing i.e. macrophage, angiogenesis and re-epithelisation. Number of macrophages and angiogenesis were analyzed using two-way ANOVA. Data of epithelial thickness were analyzed using Kruskal-Wallis test. The post hoc test in the treatment group and the control group on day 0 showed results of p = 1.00. On day 3, day 7 and day 12, the result of p was <0.05. In the treatment group on day 0 compared to the treatment group on the 3rd, 7th, 12th day, the results were p<0.05. In the treatment group on the 3rd day compared to the 7th and 12th days the results were p>0.05. The treatment group on the 7 day compared to the treatment group on the 12 day had p>0.05. In the control group, on day 0 compared to day 3, the results of p>0.05, while the control group day 0 with day 7, 12 had a result of p<0.05. The control group on the 3rd day was compared with the 7th day. The 12th also had a result of p<0.05. The comparison between the 7 day control groups showed p of >0.05. These data suggest that the papaya leaf ethanolic extract could accelerate the healing of oral ulcer on the buccal mucosa of wistar rats.

Keywords


angiogenesis; macrophage; oral ulcer; papaya leaf ethanolic extract; re-epithelisation

Full Text:

PDF


References

1. Owoyele BV, Adebukola OM, Funmilayo AA, Soladoye AO. Anti-inflammatory activities of ethanolic extract of Carica papaya leaves. Inflammopharmacology. 2008; 16 (4): 168-173. doi: 10.1007/s10787-008-7008-0

2. Conway K1, Price P, Harding KG, Jiang WG. The molecular and clinical impact
of hepatocyte growth factor, its receptor, activators, and inhibitors in wound healing. Wound Repair Regen. 2006; 14(1): 2-10. doi: 10.1111/j.1743-6109.2005.00081.x

3. Ruswanti EO, Cholil, Sukmana BI. Efektivitas ekstrak etanolik daun pepaya 100% terhadap waktu penyembuhan luka, tinjauan studi pada mukosa mulut mencit (Mus muculus). Dentino (Jur. Ked. Gigi). 2014; 2(2): 162-166.

4. Goldenberg RL. The plausibility of micronutrient deficiency in relationshipto perinatal infection. J Nutr. 2003; 133: 1645-1648. doi: 10.1093/jn/133.5.1645S

5. Kent D, Sheridan C. Choroidal neovascu-larization: a wound healing perspective. Mol Vis. 2003; 9: 747-755.

6. Park K-S, Park D-H. The effect of Korean red ginseng on full-thickness skin wound healing in rats. Journal of Ginseng Research. 2019; 43(2): 226-235. doi: 10.1016/j.jgr.2017.12.006

7. Cohen IK, Diegelmann RF, Yager DR, Wornum IL, Graham MF, Crosslland MC.
Wound care and wound healing. In: Schwartz SI, Shires GT, Spencer FC, Daly JM, Fiischer JE, Galloway AC, editor, Principles of Surgery, Ed 7. New York: Mc-Graw Hill; 1999. 225.

8. Guyton AC, Hall JE. Textbook of medical physiology. 11th Edition. Amsterdam: Elsivier Saunders. 2006. 461.

9. Lingga L. Cerdas memilih sayuran, klik publishing. PT Agro Media Pustaka; 2010. 289


10. Pramono S. Efek anti inflamasi beberapa tumbuhan umbelliferae. Hayati. 2015; 12(1): 7-10. doi: 10.1016/S1978-3019(16)30316-3

11. Harborne JB, Wiliams CA. Advances in flavonoid research since 1992. Phytochemistry. 2000; 55(6): 481-504. doi: 10.1016/s0031-9422(00)00235-1

12. Pullar JM, Carr AC, Vissers MCM. The roles of vitamin c in skin health. J Nutr. 2017; 9(8): 866. doi: 10.3390/nu9080866



DOI: https://doi.org/10.22146/majkedgiind.37026

Article Metrics

Abstract views : 5225 | views : 3776

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2019 Majalah Kedokteran Gigi Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

 View My Stats


real time web analytics